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Analysis of Cellular Components in Bacterial Classification and Diagnosis

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Gas Chromatography Mass Spectrometry Applications in Microbiology

Abstract

Bacterial cell walls have always represented a challenge to the chemist. Substances not found elsewhere in nature are frequently detected and the chemical problems of isolation, purification, and structure elucidation of bacterial compounds are both fascinating and demanding. As a result, several fundamental achievements in organic chemistry, spectroscopy, and chromatography have been carried out by chemists working on bacterial compounds, and a multitude of bacterial substances have been described over the last decades.

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References

  • Abel, K., DeSmertzing, H., and Peterson, J. I., 1963, Classification of microorganisms by analysis of chemical composition. 1. Feasibility of utilizing gas chromatography, J. Bact. 85:1039–1044.

    PubMed  CAS  Google Scholar 

  • Amdur, B. H., Szabo, E. I., and Socransky, S. S., 1978, Fatty acids of gram-positive bacterial rods from human dental plaque, Archs. Oral Biol. 23:23–29.

    Article  CAS  Google Scholar 

  • Andersen, O., Jantzen, E., Closs, O., Harboe, M., Saxegaard, F., and Fodstad, F., 1982, Fatty acid and polar lipid analysis as tools in the identification of M. leprae and some related slow-growing mycobacterial species, Ann. Microbiol. (Inst. Pasteur) 133B:29–37.

    Google Scholar 

  • Anderson, R. J., 1943, The chemistry of the lipids of the tubercle bacillus, Yale J. Biol. Med. 15:311–345.

    PubMed  CAS  Google Scholar 

  • Asselineau, J., 1966, The Bacterial Lipids ,Herman, Paris.

    Google Scholar 

  • Blaser, M. J., Moss, C. W., and Weaver, R. E., 1980, Cellular fatty acid composition of Campy`lobacter fetus, J. Clin. Microbiol. 11:448–451.

    PubMed  CAS  Google Scholar 

  • Bowie, I. S., Grigor, M. R., Dunckley, G. C., Loutit, M. V., and Loutit, J. S., 1972, The DNA base composition and fatty acid constitution of some gram-positive pleomorphic soil bacteria. Soil Biol., Biochem. 4:397–412.

    Article  CAS  Google Scholar 

  • Brennan, P. J., Mayer, H., Aspinall, G. O., and Shin, E. N., 1981, Structures of the glycopeptidolipid antigens from serovars in the Mycobacterium avium/Mycobacterium intracellulare/Mycobacterium scrofulacelum complex, Eur. J. Biochem. 115:7–15.

    Article  PubMed  CAS  Google Scholar 

  • Broady, K. W., Rietschel, E. T., and Lüderitz, O., 1981, The chemical structure of the lipid A component of lipopolysaccharides from Vibrio cholerae, Eur. J. Biochem. 115:463–468.

    Article  PubMed  CAS  Google Scholar 

  • Brooks, B. W., Murray, R. G. E., Johnson, J. L., Stackebrandt, E., Woese, C. R., and Fox, G. E., 1980, Red-pigmented micrococci: a basis for taxonomy, Int. J. System. Bact. 30:627–646.

    Article  Google Scholar 

  • Bryn, K. ,and Jantzen, E., 1982, Analysis of lipopolysaccharides by methanolysis, trifluoroacetylation, and gas chromatography on a fused-silica capillary column, J. Chromatog. 240:405–413.

    Article  CAS  Google Scholar 

  • Bryn, K., Jantzen, E., and Bøvre, K. ,1977, Occurrence and patterns of waxes in Neisseriaceae, J. Gen. Microbiol. 102:33–43.

    PubMed  CAS  Google Scholar 

  • Bryn, K., and Rietschel, E. T., 1978, L-2-Hydroxytetradecanoic acid as a constituent of Salmonella lipopolysaccharides (Lipid A), Eur. J. Biochem. 86:311–315.

    Article  PubMed  CAS  Google Scholar 

  • Buchanan, R. E., and Gibbons, N. E. (eds.), 1974, Bergey’s Manual of Determinative Bacteriology ,8th ed.. The Williams and Wilkins Co., Baltimore.

    Google Scholar 

  • Bøvre, K., 1980, “Neisseriaceae-Genetic Affinities” in Microbiological Classification and Identification (M. Goodfellow, and R. G. Board, eds.), pp. 189–256, Academic Press, New York.

    Google Scholar 

  • Bøvre, K., Fuglesang, J. E., Hagen, N., Jantzen, E., and Frøholm, L. O., 1976, Moraxella atlantae sp. nov. and its distinction from Moraxella phenylpyrouvica, Int. J. System. Bact. 26:511–521.

    Article  Google Scholar 

  • Bøvre, K., Hagen, N., Berdal, B. P., and Jantzen, E., 1977, Oxidase positive rods from cases of suspected gonorrhoea. A comparison of conventional, gas Chromatographic and genetic methods of identification, Ada Path. Microbiol. Scand., Sect. B 85:27–37.

    Google Scholar 

  • Chomarat, M., Flandrois, J. P., and Viallier, J., 1981, Analyse des acides gras des mycobactéries par Chromatographie gaz-liquide. Etude de M. avium et de 20 autre espéces, Zbl. Bakt. Hyg., I. Abt. Orig. C., 2:21–32.

    CAS  Google Scholar 

  • Clamp, J. R., Bhatti, T., and Chambers, R. E., 1971, “Determination of Carbohydrates in Biological Materials by Gas-Liquid Chromatography,” in Methods of Biochemical Analysis ,Vol. 19 (D. Glick, ed.), pp. 229–344, Wiley-Interscience, New York.

    Chapter  Google Scholar 

  • Cohen, P. G., Moss, C. W., and Farshthi, D., 1970, Cellular fatty acids of treponemes, Br. J. Vener. Dis. 46:10–12.

    PubMed  CAS  Google Scholar 

  • Cummins, C. S., and Harris, H., 1956, The chemical composition of the cell wall in some gram-positive bacteria and its possible value as a taxonomic character, J. Gen. Microbiol. 14:583–600.

    PubMed  CAS  Google Scholar 

  • Dees, S. B., Hollis, D. G., Weaver, R. E., and Moss, C. W., 1981, Cellular fatty acids of Brucella canis and Brucella suis, J. Clin. Microbiol. 14:111–112.

    PubMed  CAS  Google Scholar 

  • Dees, S. B., and Moss, C. W., 1975, Cellular fatty acids of Alcaligenes and Pseudomonas species from clinical specimens, J. Clin. Microbiol. 1:414–419.

    PubMed  CAS  Google Scholar 

  • Dees, S. B., and Moss, C. W., 1978, Identification of Achromobacter species by cellular fatty acid and production of keto acids, J. Clin. Microbiol. 8:61–66.

    PubMed  CAS  Google Scholar 

  • Dees, S. B., and Moss, C. W., 1978, Identification of the total cellular fatty acids of Staphylococcus species of human origin, Int. J. System. Bact. 28:223–228.

    Article  Google Scholar 

  • Dees, S. B., Moss, C. W., Weaver, R. E., and Hollis, D., 1979, Cellular fatty acid composition of Pseudomonas paucimobilis and groups IIk-2, Ve-1, and Ve-2, J. Clin. Microbiol. 10:206–209.

    PubMed  CAS  Google Scholar 

  • Dees, S. B., Thanabalasundrum, S., Moss, C. W., Hollis, D. G., and Weaver, R. E., 1980, Cellular fatty acid composition of group IVe, a nonsaccharolytic organism from clinical sources, J. Clin. Microbiol. 11:664–668.

    PubMed  CAS  Google Scholar 

  • Drücker, D. P., 1981, Microbial Application of Gas Chromatography ,Cambridge University Press, Cambridge, England.

    Google Scholar 

  • Drücker, D. P., 1976, “Gas-Liquid Chromatographie Chemotaxonomy,” in Methods of Microbiology ,Vol. 9 (J. R. Norris, ed.), pp. 51–125, Academic Press, New York.

    Google Scholar 

  • Eilender, R. D., Jr., Hidalgo, R. J., and Grumbles, L. C., 1970, Characterization of five clostridial pathogens by gas-liquid chromatography, Am. J. Vet. Res. 31:1863–1866.

    Google Scholar 

  • Elsden, S. R., Hilton, M. G., Parsey, K. R., and Self, R., 1980, The lipid fatty acids of proteolytic clostridia, J. Gen. Microbiol. 118:115–123.

    CAS  Google Scholar 

  • Ellwood, D. C., and Tempest, D. W., 1972, Effects of environment on bacterial wall content and composition, Adv. Microbial. Physiol. 7:83–117.

    Article  CAS  Google Scholar 

  • Farshy, D. C., and Moss, C. W., 1970, Characterization of clostridia by gas chromatography: differentiation of species by trimethylsilyl derivatives of whole-cell hydrolysates, Appl. Microbiol. 20:78–84.

    PubMed  CAS  Google Scholar 

  • Fautz, E., Rosenfelder, G., and Grotjahn, L., 1979, Iso-branched 2and 3-hydroxy fatty acids as characteristic lipid constituents of some gliding bacteria, J. Bact. 140:852– 858.

    PubMed  CAS  Google Scholar 

  • Fischer, W., 1977, The polar lipids of group B streptococci. II. Composition and positional distribution of fatty acids, Biochim. Biophys. Acta 487:89–104.

    PubMed  CAS  Google Scholar 

  • Fisher-Hoch, S., Hudson, M. J., and Thompson, M. H., 1979, Identification of a clinical isolate as Legionella pneumophila by gas chromatography and mass spectrometry of cellular fatty acids, Lancet ,viii:323–325.

    Article  Google Scholar 

  • Flesher, A. R., and Insel, R. A., 1978, Characterization of lipopolysaccharides of Haemophilus influenzas J. Inf. Dis. 138:719–730.

    Article  CAS  Google Scholar 

  • Fox, A., Schwab, J. H., and Cochran, T., 1980, Muramic acid detection in mammalian tissues by gas-liquid chromatography/mass spectrometry, Infect. Immun. 29:526–531.

    PubMed  CAS  Google Scholar 

  • Goldfine, H., 1972, Comparative aspects of bacterial lipids, Adv. Microbial Physiol. 8:1–57.

    Article  CAS  Google Scholar 

  • Guerrant, G. O., Lambert, M. S., and Moss, C. W., 1979, Identification of diaminopimelic acid in the Legionnaires disease bacterium, J. Clin. Microbiol. 10:815–818.

    PubMed  CAS  Google Scholar 

  • Hofstad, T., and Jantzen, E., 1982, Fatty acids of Leptotrichia buccalis: taxonomic implications, J. Gen. Microbiol. 128:151–153.

    CAS  Google Scholar 

  • Hofstad, T., and Skaug, N., 1980, Fatty acids and neutral sugars present in lipopolysaccharides isolated from Fusobacterium species, Acta Path. Microbiol. Scand., Sect. B 88:115–120.

    CAS  Google Scholar 

  • Hunter, S. W., and Brennan, P. J., 1981, A novel phenolic glycolipid from Mycobacterium leprae possibly involved in immunogenicity and pathogenicity, J. Bact. 147:728-735.

    PubMed  CAS  Google Scholar 

  • Jantzen, E., Berdal, B. P., and Omland, T., 1979, Cellular fatty acid composition of Francisella tularensis, J. Clin. Microbiol. 10:928–930.

    PubMed  CAS  Google Scholar 

  • Jantzen, E., Berdal, B. P., and Omland, T., 1980 Cellular fatty acid composition of Haemophilus species, Pasteurella multocida, Actinobacillus actinomycetemcomitans and Haemophilus vaginalis (Corynebacterium vaginale) ,Acta Path. Microbiol. Scand. Sect. B 88:89–93.

    CAS  Google Scholar 

  • Jantzen, E., Berdal, B. P., and Omland, T., 1981, “Fatty Acid Taxonomy of Haemophilus, Pasteurella ,and Actinobacillus,” in Haemophilus, Pasteurella, and Actinobacillus (M. Kilian, and W. Fredriksen, eds.), pp. 197–203, Academic Press, New York.

    Google Scholar 

  • Jantzen, E., Bergan, T., and Bøvre, K., 1974a, Gas chromatography of bacterial whole cell methanolysates. VI. Fatty acid composition of strains within Micrococcaceae, Acta Path. Microbiol. Scand., Sect. B 82:785–798.

    CAS  Google Scholar 

  • Jantzen, E., Bryn, K., Bergan, T., and Bøvre, K., 1974b, Gas chromatography of bacterial whole cell methanolystates. V. Fatty acid composition of neisseriae and moraxellae, Acta Path. Microbiol. Scand., Sect. B 82:767–779.

    CAS  Google Scholar 

  • Jantzen, E., Bryn, K., Bergan, T., and Bøvre, K., 1975, Gas chromatography of bacterial whole cell methanolystates. VII. Fatty acid composition of Acinetobacter in relation to the taxonomy of Neisseriaceae, Acta Path. Microbiol. Scand., Sect. B 83:569–580.

    CAS  Google Scholar 

  • Jantzen, E., Bryn, K., and Bøvre, K., 1974c, Gas chromatography of bacterial whole cell methanolysates. IV. A procedure for fractionation and identification of fatty acids and monosaccharides of cellular structures, Acta Path. Microbiol. Scand., Sect. B ,82:753– 766.

    CAS  Google Scholar 

  • Jantzen, E., Bryn, K., and Bøvre, K., 1976, Monosaccharide patterns of Neisseriaceae, Acta Path. Microbiol Scand., Sect. B 84:177–188.

    CAS  Google Scholar 

  • Jantzen, E., Bryn, K., Hagen, N., Bergan, T., Bøvre, K., 1978, Fatty acids and monosaccharides of Neisseriaceae in relation to established taxonomy, NIPH Annals (Oslo) 1:59–71.

    CAS  Google Scholar 

  • Jantzen, E., Frøholm, L. O., Hytta, R., and Bøvre, K., 1972, Gas chromatography of bacterial whole cell methanolysates. I. The usefulness of trimethylsilyland trifluoroacetyl derivatives for strain and species characterization, Acta Path. Microbiol. Scand., Sect. B 80:660– 671.

    CAS  Google Scholar 

  • Jantzen, E., and Hofstad, T., 1981, Fatty acids of Fusobacterium species: taxonomic implications, J. Gen. Microbiol. 123:163–171.

    PubMed  CAS  Google Scholar 

  • Jantzen, E., Knudsen, E., and Winsnes, R., 1982, Fatty acid analysis for differentiation of Bordetella and Brucella species, Acta Pathol. Microbioi Scand., Sect. B ,90:353–359.

    CAS  Google Scholar 

  • Jantzen, E., and Lassen, J., 1980, Characterization of Yersinia species by analysis of whole-cell fatty acids, Int. J. System. Bad. 30:421–428.

    Article  CAS  Google Scholar 

  • Jennings, H. J., Hawes, G. B., Adams, G. A., and Kenny, C. P., 1973, The chemical composition and serological reactions of lipopolysaccharides from serogroups A, B, X, and Y Neisseria meningitidis, Can. J. Biochem. 51:1347–1354.

    Article  PubMed  CAS  Google Scholar 

  • Julak, J.,Turecek, F., and Mikova, Z., 1980, Identification of characteristic branched-chain fatty acids of Mycobacterium kansasii and gordonae by gas chromatography-mass spectrometry, J.Chromatog. 190:183–187.

    Article  CAS  Google Scholar 

  • Kamio, Y., Kanegasaki, S. and Takahashi, H., 1969, Occurrence of plasmalogens in anaerobic bacteria, J. Gen. Microbioi. 15:439–451.

    Article  CAS  Google Scholar 

  • Kaneda, T., 1977, Fatty acids of the genus Bacillus: An example of branched-chain preference, Bact. Rev. 41:391–418.

    PubMed  CAS  Google Scholar 

  • Keddie, R. M., and Bousfield, I. J., 1980, “Cell-Wall Composition in the Classification and Identification of Coryneform Bacteria,” in Microbial Classification and Identification, Society for Applied Bacteriology (M. Goodfellow, and R. G. Board, eds.), pp. 167–187, Academic Press, New York.

    Google Scholar 

  • Keddie, R. M., and Cure, G. L., 1978, “Cell-Wall Composition of Coryneform Bacteria,” in Coryneform Bacteria ,Society for General Microbiology (I. J. Bousfield, and A. G. Calley, eds.), pp. 47–83, Academic Press, New York.

    Google Scholar 

  • Kiley, P., and Holt, S. C., 1980, Characterization of the lipopolysaccharide from Actinobacillus actinomycetemcomitans Y4 and N27, Infect. Immun. 30:862–873.

    PubMed  CAS  Google Scholar 

  • Knudsen, E., Jantzen, E., Bryn, K., Ormerod, J., and Sirevåg, R., 1982, Quantitative and structural characteristics of lipids in Chlorobium and Chloroflexus, Arch. Microbioi. 132:149–154.

    Article  CAS  Google Scholar 

  • Lambert, M. A., and Armfield, A. Y., 1979, Differentiation of Peptococcm and Peptostreptococcus by gas-liquid chromatography of cellular fatty acids and metabolic products, J. Clin. Microbioi. 10:464–476.

    CAS  Google Scholar 

  • Lambert, M. A., Hollis, D. G., Moss, C. W., Weaver, R. E., and Thomas, M. L., 1971, Fatty acid composition of nonpathogenic Neisseria, Can. J. Microbioi. 17:1491–1502.

    Article  CAS  Google Scholar 

  • Lambert, M. A., and Moss, C. W., 1976, Cellular fatty acid composition of Streptococcus mutans and related streptococci, I. Dent. Res. Spec. Iss.A. 55:A96–A102.

    CAS  Google Scholar 

  • Larsson, L., Bergman, R., and Mårdh, P.-A., 1979, Gas Chromatographie characterization of porcine and human strains belonging to the Mycobacterium avium-intracellulare complex, Acta Path. Microbioi. Scand., Sect. B 87:205–209.

    Google Scholar 

  • Larsson, L., and Mårdh, P.-A., 1976, Gas Chromatographic characterization of mycobacteria: Analysis of fatty acids and trifluoroacetylated whole-cell methanolysates, J. Clin. Microbioi. 3:81–85.

    CAS  Google Scholar 

  • Larsson, L., and Mårdh, P.-A., 1977, Application of gas chromatography to diagnosis of microorganisms and infectious diseases, Acta Path. Microbioi, Scand., Sect. B, Suppl. 259:5–15.

    CAS  Google Scholar 

  • Larsson, L., Mårdh, P.-A., and Odham, G., 1979, Detection of tuberculostearic acid in mycobacteria and nocardia by gas chromatography and mass spectrometry using selected monitoring, I. Chromatog. 163:221–224.

    CAS  Google Scholar 

  • Larsson, L., Mårdh, P.-A., Odham, G., and Westerdahl, G., 1980, Detection of tuberculostearic acid in biological specimens by means of glass capillary gas chromatographyelectron and chemical ionization mass spectrometry, utilizing selected ion monitoring, J. Chromatog. 182:402–408.

    Article  CAS  Google Scholar 

  • Larsson, L., Mårdh, P.-A., Odham, G., and Westerdahl, G., 1981, Use of selected ion monitoring for detection of tuberculostearic and C32 mycocerosic acid in mycobacteria and in five-day-old cultures of sputum specimens from patients with pulmonary tuberculosis, Acta Path. Microbiol. Scand. Sect. B 89:245–251.

    CAS  Google Scholar 

  • Lechevalier, M. P., 1977, Lipids in bacterial taxonomy-A taxonomist’s viewpoint, Crit. Rev. Microbiol. 5:109–210.

    Article  CAS  Google Scholar 

  • Le Dur, A., Caroff, M., Chaby, R., and Szabo, L., 1980, A novel type of endotoxin structure present in Bordetella pertussis. Isolation of two different polysaccharides bound to Lipid A, Eur. J. Biochem. 84:579–589.

    Article  Google Scholar 

  • Livermore, B. P., and Johnson, R. C., 1974, Lipids of the Spirochaetales, Spirochaeta, Treponema ,and Leptospira, J. Bact. 120:1268–1273.

    PubMed  CAS  Google Scholar 

  • Lüderitz, O., Westphal, O., Staub, A. M., and Nikaido, H., 1971, “Isolation and Chemical and Immunological Characterization of Bacterial Lipopolysaccharides,” in Microbial Toxins ,Vol. IV (G. Weinbaum, S. Kadis, and S. Ajl, eds.), pp. 145–224, Academic Press, New York.

    Google Scholar 

  • Lugowski, C., and Romanowska, E. ,1974, Chemical studies on Shigella sonnei lipid A, Eur. J. Biochem. 48:319–323.

    Article  PubMed  CAS  Google Scholar 

  • Maitra, S. K., Scholtz, M. C., Yoshikawa, T. T., and Guze, L. B., 1978, Determination of lipid A and endotoxin in serum by mass spectrometry, Proc. Natl. Acad. Sci. USA 75:3993–3997.

    Article  PubMed  CAS  Google Scholar 

  • Mandel, M., 1969, New approaches to bacterial taxonomy: Perspective and prospects, Ann. Rev. Microbiol. 23:239–274.

    Article  CAS  Google Scholar 

  • Matthews, H., Yang, T.-K., and Jenkin, H., 1979, Unique lipid composition of Treponema pallidum (Nichols virulent strain), Inf. Immun. 24:713–719.

    CAS  Google Scholar 

  • Mayberry, W. R., 1980, Cellular distribution and linkage of D-(-)-3-hydroxy fatty acids in Bacteroides species, J. Bact. 144:200–204.

    PubMed  CAS  Google Scholar 

  • Mayberry, W. R., 1981, Dihydroxy and monohydroxy fatty acids in Legionella pneumophila, J. Bact. 147:373–381.

    PubMed  CAS  Google Scholar 

  • Minnikin, D. E., and Goodfellow, M., 1980, “Lipid Composition in the Classification and Identification of Acid-Fast Bacteria”’ in Microbiological Classification and Identification, Society for Applied Bacteriology (M. Goodfellow, and R. G. Board, eds.), pp. 189–256, Academic Press, New York.

    Google Scholar 

  • Minnikin, D. E., Goodfellow, M., and Collins, M. D., 1978. “Lipid Composition in the Classification and Identification of Coryneform and Related Taxa,” in Coryneform Bacteria ,Society for General Microbiology (I. J. Bousfield, and A. G. Calley, eds.), pp. 84–160, Academic Press, New York.

    Google Scholar 

  • Miyagawa, E., and Suto, T., 1980, Cellular fatty acid composition in Bacteroides oralis and Bacteroides ruminicola, J. Gen. Appl. Microbiol. 26:331–343.

    Article  CAS  Google Scholar 

  • Mitruka, B. M., 1974, Gas Chromatographic Applications in Microbiology and Medicine ,John Wiley and Sons, New York.

    Google Scholar 

  • Moreno, E., Pitt, M. W., Jones, L. M., Schurig, G. G., and Berman, D. T., 1979, Purification and characterization of smooth and rough lipopolysaccharides from Brucella abortus, J. Bact. 138:361–369.

    PubMed  CAS  Google Scholar 

  • Morrison, S. J., Tornabene, T. G., and Kloos, W. E., 1971, Neutral lipids in the study of relationships of members of the family Micrococcaceae, J. Bact. 108:353–358.

    PubMed  CAS  Google Scholar 

  • Moss, C. W., 1978, “New Methodology for Identification of Nonfermenters: Gas-Liquid Chromatographie Chemotaxonomy,” in Glucose Nonfermenting Gram-Negative Bacteria in Clinical Microbiology (G. L. Gilardi, ed.), pp. 171–201, CRC Press, Boca Raton, Florida.

    Google Scholar 

  • Moss, C. W., 1981, Gas-liquid chromatography as an analytical tool in microbiology, J. Chromatog. 203:337–347.

    Article  CAS  Google Scholar 

  • Moss, C. W., and Dees, S. B., 1976, Cellular fatty acids and metabolic products of Pseudomonas species obtained from clinical specimens, J. Clin. Microbiol. 4:492–502.

    PubMed  CAS  Google Scholar 

  • Moss, C. W., Dowell, V. R., Farshtchi, D., Raines, L. J., and Cherry, W. B., 1969, Cultural characteristics and fatty acid composition of propionibacteria, J. Bact. 97:561–570.

    PubMed  CAS  Google Scholar 

  • Moss, C. W., Dowell, V. R., Lewis, J. T., and Schekter, M. A., 1967, Cultural characteristics and fatty acid composition of Corynebacterium acnes, J. Bact. 94:1300–1305.

    PubMed  CAS  Google Scholar 

  • Moss, C. W., and Dunkelberg, W. E., 1969, Volatile and cellular fatty acids of Haemophilus vaginalis, J. Bact. 10:544–546.

    Google Scholar 

  • Moss, C. W., Lambert, M. A., and Merwin, W., 1974, Comparison of rapid methods for analysis of bacterial fatty acids, Appl. Microbiol. 28:80–85.

    PubMed  CAS  Google Scholar 

  • Moss, C. W., Thomas, M. L., and Lambert, M. A., 1971, Amino acid composition of treponemes, Br. J. Verier. Dis. 47:165–168.

    CAS  Google Scholar 

  • Moss, C. W., Weaver, R. E., Dees, S. B., and Cherry, W. B., 1977, Cellular fatty acid composition of isolates from Legionnaires disease, J. Clin. Microbiol. 6:140–143.

    PubMed  CAS  Google Scholar 

  • Niskanen, A., Kiutamo, T., Raisanen, S., and Raevuori, M., 1980, Determination of fatty acid composition of Bacillus cereus and related bacteria: a rapid gas Chromatographie method using glass capillary column, Appl. Environ. Microbiol. 35:453–455.

    Google Scholar 

  • Odham, G., Larsson, L., and Mårdh, P.-A., 1979, Determination of tuberculostearic acid in sputum from patients with pulmonary tuberculosis by selected ion monitoring, J. Clin. Invest. 63:813–819.

    Article  PubMed  CAS  Google Scholar 

  • Oyaizu, H., and Komagata, K., 1981, Chemotaxonomic and phenotypic characterization of the strains of species in the Flavobacterium-Cytophaga complex, J. Gen. Microbiol. 27:57–107.

    Article  CAS  Google Scholar 

  • Raines, L. J., Moss, C. W., Farshtchi, D., and Pittman, B., 1968, Fatty acids of Listeria monocytogenes, J. Bact. 96:2175–2177.

    PubMed  CAS  Google Scholar 

  • Pritchard, D. G., Coligan, J. E., Speed, S. E., and Gray, B. M., 1981, Carbohydrate fingerprints of streptococcal cells, J. Clin. Microbiol. 13:89–92.

    PubMed  CAS  Google Scholar 

  • Rietschel, E. T., Gottert, H., Lüderitz, O., and Westphal, O., 1972, Nature and linkages of the fatty acids present in the lipid-A component of Salmonella lipopolysaccharides, Eur. J. Biochem. 28:166–173.

    Article  PubMed  CAS  Google Scholar 

  • Rietschel, E. T., and Lüderitz, O., 1980, Struktur von Lipopolysaccharid und Taxonomie gram-negativer Bakterien, Forum Microbiol. 1/80:12–20.

    Google Scholar 

  • Schleifer, K. H., and Kandier, O., 1972, Peptidoglycan types of bacterial cell walls and their taxonomie implications, Bact. Rev. 36:407–477.

    PubMed  CAS  Google Scholar 

  • Shaw, N., 1974, Lipid composition as a guide to the classification of bacteria, Adv. Appl. Microbiol. 17:63–108.

    Article  PubMed  CAS  Google Scholar 

  • Stocker, B. A. D., and Mäkelä, P. H., 1971, Genetic Aspects of Biosynthesis and Structure of Salmonella Lipopolysaccharides,,, in Microbiol Toxins ,Vol. IV (G. Weinbaum, S. Kadis, and S. J. Ajl, eds.), pp. 369–438, Academic Press, New York.

    Google Scholar 

  • Sud, I. J., and Feingold, D. S., 1979, Detection of 3-hydroxy fatty acids at picogram levels in biologic specimens. A chemical method for the detection of Neisseria gonorrhoeael J. Investig. Dermat. 73:521–525.

    Article  CAS  Google Scholar 

  • Tabor, M. W., MacGee, J. and Holland, J. W., 1976, Rapid determination of dipicolinic acid in the spores of Clostridium species by gas-liquid chromatography, Appl. Environ. Microbiol. 31:25–28.

    PubMed  CAS  Google Scholar 

  • Tadayon, R. A., and Carrol, K. K., 1971, Effect of growth conditions on the fatty acid composition of Listeria monocytogenes and comparison with the fatty acids of Erysipeloth rix and Corynebacterium, Lipids 6:820–825.

    Article  PubMed  CAS  Google Scholar 

  • Thoen, C. O., Karlson, A. G., and Ellefson, R. D., 1971, Comparison by gas-liquid chromatography of the fatty acids of Mycobacterium avium and some other photochromogenic mycobacteria, Appl. Microbiol. 22:560–563.

    PubMed  CAS  Google Scholar 

  • Thoen, C. O., Karlson, A. G., and Ellefson, R. D., 1973, Differentiation between Mycobacterium kansasii and Mycobacterium marinum by gas-liquid Chromatographic analysis of cellular fatty acids, Appl. Microbioi 24:1009–1010.

    Google Scholar 

  • Tisdall, P. A., De Young, D. R., Roberts, G. D., and Anhalt, J. P., 1982, Identification of clinical isolates of mycobacteria by gas-liquid chromatography alone: A 10-month follow-up study. . Clin. Microbioi 16:400–402.

    CAS  Google Scholar 

  • Tisdall, P. A., Roberts, G. D., and Anhalt, J. P., 1979, Identification of clinical isolates of mycobacteria with gas-liquid chromatography alone, J. Clin. Microbioi. 10:506–514.

    CAS  Google Scholar 

  • Tornabene, T. G., Gelpi, E., and Oro, J., 1967, Identification of fatty acids and aliphatic hydrocarbons in Sarcina lutea by gas chromatography and combined gas chromatography-mass spectrometry, J. Bact. 94:333–343.

    PubMed  CAS  Google Scholar 

  • Uchida, K., and Mogi, K., 1972, Cellular fatty acid spectra of Pediococcus species in relation to their taxonomy, J. Gen. Appl. Microbioi. 18:109–129.

    Article  CAS  Google Scholar 

  • Vaczi, L., Redai, I., and Rethy, A., 1967, Changes in the fatty acid composition of Staphylococ cus aureus under various cultural conditions, Acta Microbioi. Acad. Sci. Hung. 14:293– 298.

    CAS  Google Scholar 

  • Veerkamp, J. H., 1971, Fatty acid composition of Bifidobacterium and Lactobacillus strains, J. Bact. 108:861–867.

    PubMed  CAS  Google Scholar 

  • Wexler, H., and Oppenheim, J. D., 1980, “Listerial LPS: An Endotoxin from a Gram-Positive Bacterium,” in Bacterial Endotoxins and Host Response (M. K. Agarwal, ed.), pp. 27–50, Elsevier/North-Holland Biomedical Press, Amsterdam.

    Google Scholar 

  • Wilkinson, S. G., 1977, “Composition and Structure of Bacterial Lipopolysaccharides,” in Surface Carbohydrates of the Procaryotic Cell (I. W. Sutherland, ed.) pp. 97–175, Academic Press, New York.

    Google Scholar 

  • Zanetta, J. P., Breckenridge, W. C., and Vincendon, G., 1972, Analysis of monosaccharides by gas-liquid chromatography of the O-methyl glycosides as trifluoroacetate derivatives. Application to glycoproteins and glycolipids, J. Chromatogr. 69:291–304.

    Article  PubMed  CAS  Google Scholar 

  • Yamakawa, T., and Ueta, N., 1964, Gas Chromatographie studies of microbial components. I. Carbohydrate and fatty acid constitution of Neisseria, Jpn. J. Exp. Med. 34:361–374.

    PubMed  CAS  Google Scholar 

  • Yano, I., Ohno, Y., Masui, M., Kato, K., Yabuuchi, E., and Ohyama, A., 1976, Occurrence of 2and 3-hydroxy fatty acids in high concentrations in the extractable and bound lipids of Flavobacterium meningosepticum and Flavobacterium lib, Lipids 11:685–688.

    Article  PubMed  CAS  Google Scholar 

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© 1984 Plenum Press, New York

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Jantzen, E. (1984). Analysis of Cellular Components in Bacterial Classification and Diagnosis. In: Odham, G., Larsson, L., Mårdh, PA. (eds) Gas Chromatography Mass Spectrometry Applications in Microbiology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4526-8_9

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  • DOI: https://doi.org/10.1007/978-1-4684-4526-8_9

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