Skip to main content
SpringerLink
Log in

Immunodetection of Choriogonadotropin-Like Antigen in Bacteria Isolated from Cancer Patients

  • Chapter
Book cover Chorionic Gonadotropin

Abstract

As early as 1948, Virginia (Wuerthele-Caspe) Livingston and her associates have published a series of papers describing a pleomorphic, acid-fast bacterium which they isolated from cancer tissues and body fluids of patients with malignant neoplasms. Livingston and Alexander-Jackson (1970) published a full description of the organism(s) and proposed the name Progenitor cryptocides as a new taxon within the Actinomycetales. They reported that this bacterium crossreacts antigenically with Mycobacterium tuberculosis, with which it shares the property of acid fastness.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Acevedo, H. F.., Slifkin, M., Pouchet, G. R., and Rakhshan, M., 1977, Identification of the 6-subunit of choriogonadotropin (CG) in human spermatozoa, in The Testis in Normal and Infertile Men, P. Troen and H. R. Nankin, ed., pp. 185–192, Raven Press, N.Y.

    Google Scholar 

  • Acevedo, H. F., Slifkin, M., Pouchet, G. R., and Rakhshan, M., 1978a, Human chorionic gonadotropin in cancer cells. I. Identification in in vitro and in vivo cancer cell systems, in Detection and Prevention of Cancer, Part 2, Vol. I, H. E. Nieburns, ed., pp. 937–963, Marcel Dekker, Inc., New York.

    Google Scholar 

  • Acevedo, H. F., Slifkin, M., Pardo, M., and Pouchet, G. R., 1978b, Choriogonadotropin (CG)-like antigen in rat prostatic carcinoma: Immunohistochemical detection and fine structure studies, Prog. 3rd Internatl. Conf. on Differentiation and Neoplasia, Minneapolic, MN, Aug. 28-Sept. 1, Abst. 124.

    Google Scholar 

  • Acevedo, H. F., Slifkin, M., Pouchet, G. R., and Pardo, M., 1978c, Immunohistochemical localization of a choriogonadotropin-like protein in bacteria isolated from cancer patients, Cancer 41: 1217.

    Article  CAS  Google Scholar 

  • Acevedo, H. F., Slifkin, M., Pouchet-Melvin, G. R., and Campbell-Acevedo, E. A., 1979, Choriogonadotropin-like antigen in the anaerobic bacterium, Eubacterium Zentum, isolated from rectal tumor, Infection and Immunity 24:920.

    PubMed  CAS  Google Scholar 

  • Adejuwon, C. A., Segal, S. J., and Mitsudo, S., 1979, Protease inhibitor abolishes the apparent hCG-like immunoactivity in human tissues, Prog. of the 61st Annual Mtg., The Endocrine Society, Anaheim, CA, June 13–15, Abst. 954.

    Google Scholar 

  • Affronti, L. F., Grow, L., and Begell, F., 1975, Characterization of bacterial tumor isolates, Fed. Proc. 34(3); 1043, Abst. 4676.

    Google Scholar 

  • Affronti, L. F., Grow, L., Brumbaugh, R. and Orton, K., 1976, Bacterial tumor isolates, Abst. Anl. Mtg. Am. Soc. Microbiol., Atlantic City, NJ, May 3–8, Abst. E-56.

    Google Scholar 

  • Ankerst, J., Christensen, P., Kjellen, L., and Kronvall, G., 1974, A routine diagnostic test for IgA and IgM antibodies to Rubella virus: Absorption of IgG with Staphylococcus aureus, J. Infect. Dis. 130:268.

    Article  PubMed  CAS  Google Scholar 

  • Aoki, T., Kvedar, J. P., Hollis, V. W. Jr., and Bushar, G. S., 1976, Streptococcus pyogenes preparation OK-432: Immunoprophylactic and immunotherapeutic effects on the incidence of spontaneous leukemia in AKR mice, J. Natl. Cancer Inst. 56:687.

    PubMed  CAS  Google Scholar 

  • Asch, R. H., Fernandez, E. O., and Pauerstein, C. J., 1977, Immunodectection of hCG-like substance in human sperm, Fertility and Sterility 28:1258.

    PubMed  CAS  Google Scholar 

  • Asch, R. H., Fernandez, E. O., Magnasco, L. A., and Pauerstein, C. J., 1978, Demonstration of a chorionic gonadotropin-like substance in rabbit morulae, Fertility and Sterility 29:444.

    PubMed  CAS  Google Scholar 

  • Asch, R. H., Fernandez, E. O., Siler-Khodr, T. M., and Pauerstein, C. J., 1979: Presence of an hCG-like substance in human sperm. Am. J. Obstet. Gynec. In press.

    Google Scholar 

  • Bain, J., and Ezrin, C., 1970, Imunofluorescent localization of the LH of the human adenophypophysis, J. Clin. Endocr. 30:181.

    Article  PubMed  CAS  Google Scholar 

  • Baird-Parker, A. C., 1972, Classification and identification of Staphlococci and their resistance to physical agents, in The Staphlococci, J. O. Cohen, ed., pp. 1–20, John Wiley and Sons, Inc., New York.

    Google Scholar 

  • Baird-Parker, A. C., 1974, The basis for the present classification of staphylococci and micrococci, Ann. N.Y. Acad. Sci. 236:7.

    Article  PubMed  CAS  Google Scholar 

  • Baldwin, R. W., and Pimm, M. V., 1978, BCG in tumor immunotherapy, Adv. Cancer Res. 28:91.

    Article  PubMed  CAS  Google Scholar 

  • Beck, J. S., Porteous, I. B., and Ullyot, J. L., 1970, Gonadotropinsecreting bronchial carcinoma: Aberrant endocrine activity or trophoblastic differentiation? J. Path. 101:59.

    Article  PubMed  CAS  Google Scholar 

  • Braunstein, G. D., Vaitukaitis, J. L., Carbone, P. P., and Ross, G. T., 1973, Ectopic production of human chorionic gonadotropin by neoplasms. Ann. Int. Med. 78:39.

    PubMed  CAS  Google Scholar 

  • Channing, C. P., Stone, S. L., Sakai, C. N., Haour, F., and Saxena, B. B., 1978, A Stimulating effect of the fluid from preimplantation rabbit blastocysts upon luteinization of monkey granulosa cell cultures, J. Reprod. Fert. 54:215

    Article  CAS  Google Scholar 

  • Chou, J. Y., 1978, Regulation of the synthesis of human chorionic gonadotropin by strains of HeLa Cells in culture, In Vitro 14:775.

    Article  PubMed  CAS  Google Scholar 

  • Cohen, H. and Strampp, A., 1976, Bacterial synthesis of substance similar to human chorionic gonadotropin, Proc. Soc. Exp. Biol. Med. 152:408.

    PubMed  CAS  Google Scholar 

  • Crispen, R. G., 1974, BCG vaccine in perspective, Seminars in Oncology 1:311.

    PubMed  CAS  Google Scholar 

  • Cumins, C. S., and Linn, D. M., 1977, Reticulostimulating properties of killed vaccines of anaerobic Coryneforms and other organisms. J. Natl. Cancer Inst. 59:1679.

    Google Scholar 

  • Dailey, J. E., and Marcuse, P. M., 1969, Gonadotropin secreting giant cell carcinoma of the lung, Cancer 24:388.

    Article  PubMed  CAS  Google Scholar 

  • DeLellis, R. A., Sternberger, L. A., Mann, R. B., Banks, P. M., and Nakane, P. K., 1979, Immunoperoxidase techniques in diagnostic pathology, Report of a Workshop sponsored by the National Cancer Institute, Am. J. Clin. Path. 71:483.

    PubMed  CAS  Google Scholar 

  • Diller, I. C., and Donnelly, A. J., 1970, Experiments with mammalian tumor isolates, Ann. N.Y. Acad. Sci. 174:655.

    Article  PubMed  CAS  Google Scholar 

  • Dufau, M. L., Tsuruhara, T., and Catt, K. J., 1972a, Interaction of glycoprotein hormones with Agarose-Concanavalin-A, Biochem. Biophys. Acta 278:281

    CAS  Google Scholar 

  • Dufau, M. L., Catt, K. J., and Tsuruhara, T., 1972b, Sensitive gonadotropin responsive system and radioimmunoassay of testosterone production by the rat testis in vitro, Endocrinology 90:1032.

    Article  CAS  Google Scholar 

  • Forsgren, A., and Sjoquist, J., 1966, “Protein A” from S. Aureus. I. Pseudo-immune reation with human famma globulin, J. Immunol. 97:822.

    PubMed  CAS  Google Scholar 

  • Ghosh, N. K., and Cos, R. P., 1976, Production of human chorionic gonadotropin in HeLa cell cultures, Nature 259:416.

    Article  PubMed  CAS  Google Scholar 

  • Goldstein, D. P., 1976, Chorionic Gonadotropin, Cancer 38:453.

    Article  PubMed  CAS  Google Scholar 

  • Hattori, M., Fukase, M., Yoshimi, H., Matsukura, S. and Imura, H., 1978, Ectopic production of human chorionic gonadotropin in malignant tumors, Cancer 42:2328.

    Article  PubMed  CAS  Google Scholar 

  • Holdeman, L. V., and Moore, W. E. C. (ed.), 1972, Anaerobic Laboratory Manual, The Virginia Polytechnic Institute and State University Anaerobic Laboratory, Blacksburg, Va.

    Google Scholar 

  • Hugh, R., 1959, Motile streptococci isolated from the oro-pharyngeal region, Can. J. Microbiol. 5:351.

    CAS  Google Scholar 

  • Hugh, R., and Ryschenkow, E., 1961, Pseudomonas maZtophilia, an alcaligenes-like species, J. Gen. Microbiol. 26:123.

    PubMed  CAS  Google Scholar 

  • Kimura, I., Ohnoshi, T., Yashara, S., Sugiyama, M., Urabe, Y., Jujii, M., and Machida, Kr., 1976, Immunochemotherapy in human lung cancer using the streptococcal agent OK-432, Cancer 27:2201.

    Article  Google Scholar 

  • Kronvall, G., and Williams, R. C., Jr., 1969, Differences in antiprotein A activity among IgG subgroups, J. Immunol. 103:828.

    PubMed  CAS  Google Scholar 

  • Kurman, R. J., Scardino, P. T., McIntire, K. R., Waldemann, T. A., and Javadpour, N., 1977, Cellular localization of a-fetoprotein and human chorionic gonadotropin in germ cell tumors of the testis using an indirect immunoperoxidase technique. A new approach to classification utilizing tumor markers, Cancer 40: 2136.

    Article  PubMed  CAS  Google Scholar 

  • Kurowsky, A., Markel, D. E., Peterson, J. W., and Fitch, W. M., 1977, Primary structure of cholera toxin ß-chain. A glycoprotein hormone analog? Science 195:299.

    Article  Google Scholar 

  • LeLeux, P., and Robin, C., 1971, Immunohistochemistry of individual adenohypophyseal cells, Acta endocr. (Kbh) Supp. 153:168.

    CAS  Google Scholar 

  • Lind, I., and Mansa, B., 1968, Further investigation of specific and non-specific adsorption of serum globulins to Staphylococcus aureus. Acta Pâthol. Microbiol. Scand. (B). 73:637.

    Article  PubMed  CAS  Google Scholar 

  • Livingston, W-C V., and Alexander-Jackson, E., 1970, A specific type of organism cultivated from malignancy: bacteriology and proposed classification, Ann. N.Y. Acad. Sci. 174:636.

    Article  PubMed  CAS  Google Scholar 

  • Livingston, W-C V., and Livingston, A. M., 1974, Some cultural immunological and biochemical properties of Progenitor cryptocides, Trans. N.Y. Acad. Sci. 36:569.

    PubMed  CAS  Google Scholar 

  • Malkin, A., Kellen, J. A., Kolin, A., Cameron, R., and Farber, E., 1978, The immunohistochemical detection of chorionic gonadotropin in experimental rat hepatomas, Scand. J. Immunol. 8:Suppl. 8, 603.

    Article  CAS  Google Scholar 

  • Maruo, T., Cohen, H., and Koide, S. S., 1979, Studies on choriogonadotropin from a microorganism, Prog. of the 61st Annual Mtg., The Endocrine Society, Anaheim, Calif., June 13–15, Abst. 951.

    Google Scholar 

  • Mazurkiewicz, J. E., and Nakane, P. K., 1972, Light and electron microscopic localization of antigens in tissue embedded in polyethyleneglycol with a perioxidase-labeled antibody method, J. Histochem. Cytochem. 20:969.

    Article  PubMed  CAS  Google Scholar 

  • McBride, J. H., and Truong, K., 1978, Further comments on estimating choriogonadotropin in serum, Clin. Chem. 24:2207.

    PubMed  CAS  Google Scholar 

  • McLean, I. W., and Nakane, P. K., 1974, Periodate-Lysine-Paraformaldehyde fixative: A new fixative for immunoelectronmicroscopy, J. Histochem. Cytochem. 22:1077.

    Article  PubMed  CAS  Google Scholar 

  • McManus, L. M., Naughton, M. A., and Martinex-Hernandez, A., 1976, Human chorionic gonadotropin in human neoplastic cells, Cancer Res. 36:3476.

    PubMed  CAS  Google Scholar 

  • Moriarty, G. C., Moriarty, D. M., and Sternberger, A., 1973, Ultrastructural immunocytochemistry with unlaveled antibodies and the peroxidase-antiperoxidase couples. A technique more sensitive than radioimmunoassay, J. Histochem. Cytochem. 21:825.

    Article  PubMed  CAS  Google Scholar 

  • Naughton, M. A., Merrill, D. A., McManus, L. M., Fink, L. M., Berman, E., White, M.J., and Martinez-Hernandez, A., 1975, Localization of the ß-chain of human chorionic gonadotropin in human tumor cells and placental cells, Cancer Res. 35:1887.

    PubMed  CAS  Google Scholar 

  • Perlin, E., Engeler, J. E., Edson, J., Karp, D., McIntire, R., and Waldman, T. A., 1976, The value of serial measurement of both human chorionic gonadotropin and alpha-fetoprotein for monitoring germinal cell tumors, Cancer 37:215.

    Article  PubMed  CAS  Google Scholar 

  • Petrusz, P., 1972, Light microscopic localization of binding sites for human chorionic gonadotropin in luteinized rat ovaries by a peroxidase-labeled antibody method, J. Histochem. Cytochem. 21:279.

    Article  Google Scholar 

  • Tabson, A. S., Rosen, S. W., Tashjian, A. H., Jr., and Weintraub, B. D., 1973, Production of human chorionic gonadotropin in vitro by a cell line derived from a carcinoma of the lung, J. Nati. Cancer Inst. 50:669.

    Google Scholar 

  • Richert, N. D., and Ryan, R. J., 1977a, Specific gonadotropin binding to Pseudomonas maltophilia, Proc. Natl. Acad. Sci. U. S. A. 74:878.

    Article  CAS  Google Scholar 

  • Richert, N. D., and Ryan, R. J., 1977b, A new bacterial stimulant of rat ovarian adenylate cyclase activity. Prog. 58th Annual Meet. The Endocrine Society, Chicago, Ill., June 8–10, Abst. 85.

    Google Scholar 

  • Richert, N. D., and Ryan, R. J., 1977c, Protease inhibitors block hormonal activation of adenylate cyclase, Biochem. Biophys. Res. Comm. 78:799.

    Article  CAS  Google Scholar 

  • Richert, N. D., and Ryan, R. J., 1977d, Proteolytic enzyme activation of rat ovarian adenylate cyclase. Proc. Natl. Acad. Sci. U. S. A. 74:4857.

    Article  CAS  Google Scholar 

  • Samaan, N. A., Smith, J. P., Rutledge, F. N., and Schulta, P. N., 1976, The significance of measurement of human placental lactogen, human chorionic gonadotropin and carcinoembryonic antigen in patients with ovarian carcinoma, Am. J. Obstet. Gynec. 126:186.

    PubMed  CAS  Google Scholar 

  • Saxena, B. B., Hasan, S. H., Haour, F., and Schmidt-Gollwitzer, M., 1974, Radioreceptor assay of human chorionic gonadotropin: Detection of early pregnancy, Science 184:793.

    Article  PubMed  CAS  Google Scholar 

  • Schlegel-Haueter, S., Robinson, J. D., and Cho, J. Y., 1978, Characterization of human chorionic gonadotropin synthesized by human cell lines, Prog. 60th Annual Meet., The Endocrine Society, Miami, FA, June 14–16, Abst. 43.

    Google Scholar 

  • Slifkin, M., Acevedo, H. F., Pardo, M., Pouchet, G. R., and Rakshan, M., 1978, Human chorionic gonadotropin in cancer cells. II. Ultrastructural localization, in Detection and Prevention of Cancer, Part 2, Vol. I, H. E. Nieburgs, ed., pp. 965–979, Marcel Dekker, Inc., New York.

    Google Scholar 

  • Slifkin, M., Pardo, M., Pouchet-Melvin, G. R., and Acevedo, H. F., 1979: Immunoelectronmicroscopic localization of a choriogonadotropin-like antigen in cancer associated bacteria, Oncology 36:208.

    Article  PubMed  CAS  Google Scholar 

  • Streefkerk, J. C., 1972, Inhibition of erythrocyte pseudoperoxidase activity by treatment with hydrogen peroxide following methanol, J. Histochem. Cytochem. 20:829.

    Article  PubMed  CAS  Google Scholar 

  • Taylor, C. R., 1978, Immunohistological approach to tumor diagnosis, Oncology 35:189.

    Article  Google Scholar 

  • The American Type Culture Collection, Catalog of Strains, 13th Edition, 1978, H. D. Hatt and M. J. Gantt, ed., Rockville, Md.

    Google Scholar 

  • Tormey, D. C., Waalkes, T. P., Ahman, D., Gehrke, C. W., Zumwatt, R. W., Snyder, J., and Hansen, H., 1975, Biological markers in breast carcinoma. I. Incidence of abnormalities of CEA, hCG, three polyamines and three minor nucleosides, Cancer 35: 1095.

    Article  PubMed  CAS  Google Scholar 

  • Vaitukaitis, J. L., Braunstein, G. D., and Ross, G. T., 1972, A radioimmunoassay which specifically measures human chorionic gonadotropin in the presence of human luteinizing hormone, Am. J. Obstet. Gynec. 113:751.

    PubMed  CAS  Google Scholar 

  • Van Larabeke, N., Genetello, Ch., Schell, J., Schilperoort, R. A., Hermans, A. K., Hernalsteens, J. P., and Van Montagu, M., 1975, Acquisition of tumor-inducing ability by non-oncogenic Agro-bacteria as a result of plasmid transfer, Nature 255:742.

    Article  Google Scholar 

  • Washington, J. A., II, Martin, W. J., and Karlson, A. G., 1974, Identification of bacteria, in Laboratory Procedures in Clinical Microbiology, J. A. Washington, II, ed., pp. 53–124, Little, Brown and Co., Boston.

    Google Scholar 

  • Watson, B., Currier, T. C., Gordon, M. P., Chilton, M-D., and Nester, E. W., 1975, Plasmid required for virulence of Agrobacterium tumefaciens, J. Bact. 123:255.

    PubMed  CAS  Google Scholar 

  • Weir, E. E., Pretlow, II T. G., Pitts, A., and Williams, E. E., 1974, Determination of endogenous peroxidase activity in order to locate cellular antigens by peroxidase-labeled antibodies, J. Histochem. Cytochem. 22:55.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Experimental Pathology, Department of Laboratory Medicine, Allegheny General Hospital, Pittsburgh, Pennsylvania, 15212, USA

    Hernan F. Acevedo, Elizabeth A. Campbell-Acevedo & Malcolm Slifkin

Authors
  1. Hernan F. Acevedo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Elizabeth A. Campbell-Acevedo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Malcolm Slifkin
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. The Rockefeller Foundation, New York, New York, USA

    Sheldon J. Segal

Rights and permissions

Reprints and permissions

Copyright information

© 1980 Plenum Press, New York

About this chapter

Cite this chapter

Acevedo, H.F., Campbell-Acevedo, E.A., Slifkin, M. (1980). Immunodetection of Choriogonadotropin-Like Antigen in Bacteria Isolated from Cancer Patients. In: Segal, S.J. (eds) Chorionic Gonadotropin. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1062-4_22

Download citation

  • DOI: https://doi.org/10.1007/978-1-4684-1062-4_22

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-1064-8

  • Online ISBN: 978-1-4684-1062-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation