Skip to main content
SpringerLink
Log in

Cloning in Yeast

  • Chapter
Techniques in Molecular Biology

Abstract

The yeast Saccharomyces cerevisiae was a logical choice for the extension of the pioneering work on gene cloning in Escherichia coli to eukaryotic organisms. As a single-celled microbe it can be cultured and manipulated using the standard techniques applied to bacteria. Nonetheless, it is a fully fledged eukaryote with its genetic material packaged into chromosomes within a membrane-enclosed nucleus and partitioned at cell division by mitosis and meiosis. In addition, a great wealth of knowledge has been accumulated over the many years that yeast has been used as a model system for biochemical and genetic studies.1 A comprehensive map is available showing the location of over 400 genes on 17 chromosomes.2 The long-standing role of yeast in fermentation processes provided an additional impetus for the development of a cloning system in this organism.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Further Reading

  • Rothstein, R. (1985) Cloning in Yeast, in D.M. Glover (ed.), DNA Cloning: A Practical Approach 2, pp. 45–66. (IRL Press, Oxford and Washington)

    Google Scholar 

  • Sherman, F., Fink, G.R. and Hicks, J.B. (1982) Methods of Yeast Genetics: Laboratory Manual (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York)

    Google Scholar 

References

  1. Strathern, J.N., Jones, E.W. and Broach, J.R. (1981) The Molecular Biology of the Yeast Saccharomyces: Life Cycle and Inheritance (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York)

    Google Scholar 

  2. Mortimer, R.K. and Schild, D. (1984) Genetic Map of Saccharomyces cerevisiae, in O’Brien, S.J. (ed.) Genetic Maps 1984, Volume 3, pp. 224–233. (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York)

    Google Scholar 

  3. Strahl, K., Cameron, J.R. and Davis, R.W. (1976) Functional Genetic Expression of Eukaryotic DNA in Escherichia coli, Proc. Natl. Acad. Sci. USA, 73, 1471–1475

    Article  Google Scholar 

  4. Ratzkin, B. and Carbon, J. (1977) Functional Expression of Cloned Yeast DNA in Escherichia coli, Proc. Natl. Acad. Sci. USA, 74, 487–491

    Article  Google Scholar 

  5. Hinnen, A., Hicks, J.B. and Fink, G.R. (1978) Transformation of Yeast, Proc. Natl. Acad. Sci. USA, 75, 1929–1933

    Article  Google Scholar 

  6. Beggs, J.D. (1978) Transformation of Yeast by a Replicating Hybrid Plasmid, Nature, Lond., 275, 104–109

    Article  Google Scholar 

  7. Strahl, K., Stinchcomb, D.T., Scherer, S. and Davis, R.W. (1979) High Frequency Transformation of Yeast: Autonomous Replication of Hybrid DNA Molecules, Proc. Natl. Acad. Sci. USA, 78, 167–171

    Google Scholar 

  8. Beggs, J.D. (1981) Gene Cloning in Yeast, in Williamson, R. (ed.) Genetic Engineering Volume 2, pp. 175–203. (Academic Press, London)

    Google Scholar 

  9. Ito, H., Fukada, Y., Murata, K. and Kimura, A. (1983) Transformation of Intact Yeast Cells Treated with Alkali Cations, J. Bacteriol., 153, 163–168

    Google Scholar 

  10. Henderson, R.C.A., Cox, B.S. and Tubb, R. (1985) The Transformation of Brewing Yeasts with a Plasmid Containing the Gene for Copper Resistance, Curr. Genet., 9, 133–138

    Article  Google Scholar 

  11. Hadfield, C. (1985) Manuscript in preparation

    Google Scholar 

  12. Orr-Weaver, T.L., Szostak, J.W. and Rothstein, R.J. (1981) Yeast Transformation: A Model System for the Study of Recombination, Proc. Natl. Acad. Sci. USA, 78, 6354–6358

    Article  Google Scholar 

  13. Broach, J.R. (1981) The Yeast Plasmid 2 µ Circle, in Strathern, J.N., Jones, E.W. and Broach, J.R. (eds) The Molecular Biology of the Yeast Saccharomyces: Life Cycle and Inheritance, pp. 445–470. (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York)

    Google Scholar 

  14. Broach, J.R. (1983) Construction of High Copy Number Yeast Vectors Using 2 urn Circle Sequences, in Methods in Enzymology, 101, pp. 307–324. (Academic Press, New York)

    Google Scholar 

  15. Jayaram, M, Li, Y.Y. and Broach, J.R. (1983) The Yeast Plasmid 2 ja Circle Encodes Components Required for its High Copy Propagation, Cell, 34, 95–104

    Article  Google Scholar 

  16. Kikuchi, Y. (1983) Yeast Plasmid Requires a CiS-acting Locus and Two Plasmid Proteins for Its Stable Maintenance, Cell, 35, 487–493

    Article  Google Scholar 

  17. Stinchcomb, D.T., Struhl, K. and Davis, R.W. (1979) Isolation and Characterisation of a Yeast Chromosomal Replicator, Nature, Lond., 282, 39–43

    Article  Google Scholar 

  18. Williamson, D.H. (1985) The Yeast ARS Element, Six Years On: A Progress Report, Yeast, 1, 1–14

    Article  Google Scholar 

  19. Clarke, L. and Carbon, J. (1980) Isolation of a yeast centromere and construction of functional small circular chromosomes, Nature, Lond., 287, 504–509

    Article  Google Scholar 

  20. Szostak, J.W. and Blackburn, E.H. (1982) Cloning Yeast Telomeres on Linear Plasmid Vectors, Cell, 29, 245–255

    Article  Google Scholar 

  21. Murrary, A.W. and Szostak, J.W. (1983) Construction of Artificial Chromosomes in Yeast, Nature, Lond., 300, 189–193

    Article  Google Scholar 

  22. Pringle, J.R. and Hartwell, L.H. (1981) The Saccharomyces cerevisiae Cell Cycle, in Strathern, J.N., Jones, E.W. and Broach, J.R. (eds) The Molecular Biology of the Yeast Saccharomyces: Life Cell and Inheritance, pp. 97–142. (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York)

    Google Scholar 

  23. Struhl, K. (1983) The New Yeast Genetics, Nature, Lond., 305, 391–397

    Article  Google Scholar 

  24. Davis, R.W., Thomas, H., Cameron, J. St., John, T.P. Scherer, S. and Padgett, P.A. (1980) Rapid DNA isolations for enzymatic and hybridisation analysis, in Methods in Enzymology, 65, pp. 404–411. (Academic Press, New York)

    Google Scholar 

  25. Rothstein, R.J. (1983) One-step Gene Disruption in Yeast, in Methods in Enzymology, 101, 202–211. (Academic Press, New York)

    Google Scholar 

  26. Neff, N.F., Thomas, J.H., Crisafi, P. and Botstein, D. (1983) Isolation of the ß-Tubulin Gene from Yeast and Demonstration of its Essential Function in vivo, Cell, 33, pp. 211–219

    Article  Google Scholar 

  27. Scherer, S. and Davis, R.W. (1979) Replacement of chromosome segments with altered DNA sequences constructed in vitro, Proc. Natl. Acad. Sci. USA, 76, 4951–4955

    Article  Google Scholar 

  28. Winston, F., Chumley, F. and Fink, G.R. (1983) Eviction and Transplacement of Mutant Genes in Yeast, in Methods in Enzymology, 101, 211–228. (Academic Press, New York)

    Google Scholar 

  29. Boeke, J.D., La Croûte, F. and Fink, G.R. (1984) A Positive Selection of Mutants Lacking Orotidine-5-phosphate Decarboxylase Activity in Yeast: 5 Fluoro-orotic Acid Resistance, Molec. Gen. Genet., 197, 345–346

    Article  Google Scholar 

  30. Orr-Weaver, T.L., Szostak, J.W. and Rothstein, R.J. (1983) Genetic Applications of Yeast Transformation with Linear and Gapped Plasmids, in Methods in Enzymology, 101, pp. 228–245. (Academic Press, New York)

    Google Scholar 

  31. Orr-Weaver, T.L. and Szostak, J.W. (1983) Yeast Recombination: The Association Between Double-strand Gap Repair and Crossing-over, Proc. Natl. Acad. Sci. USA, 80, 4417–4421

    Article  Google Scholar 

  32. Beggs, J.D., van den Berg, J., van Ooyen, A. and Weissmann, C. (1980) Abnormal Expression of Chromosomal Rabbit ß-globin Gene in Saccharomyces cerevisiae, Nature, Lond., 283, 835–840

    Article  Google Scholar 

  33. Tuite, M.F., Dobson, M.J., Roberts, N.A., King, R.M., Burke, D.C., Kingsman, S.M. and Kingsman, A.J. (1982) Regulated High Efficiency Expression of Human Interferon-alpha in Saccharomyces cerevisiae, EMBO J., 1, 603–608

    Google Scholar 

  34. Hitzeman, R.A., Hagie, F.E., Levine, H.L., Goeddel, D.V., Ammerer, G. and Hall, B.D. (1981) Expression of a Human Gene for Interferon in Yeast, Nature, Lond., 293, 717–723

    Article  Google Scholar 

  35. Derynck, R., Singh, H. and Goedell, D.V. (1983) Expression of the Human Interferon α-cDNA in Yeast, Nucleic Acids Res., 11, 1817–1837

    Article  Google Scholar 

  36. Mellor, J., Dobson, M.J., Roberts, N.A., Tuite, M.F., Emtage, J.S., White, S., Lowe, P.A., Patel, T., Kingsman, A.J. and Kingsman, S.M. (1983) Efficient Synthesis of Enzymatically Active Calf Chymosin in Saccharomyces cerevisiae, Gene, 24, 1–14

    Article  Google Scholar 

  37. Kramer, R.A., De Chiara, T.M., Schaber, M.D. and Hilliker, S. (1984) Regulated Expression of a Human Interferon Gene in Yeast: Control by Phosphate Concentration or Temperature, Proc. Natl. Acad. Sci. USA, 81, 367–370

    Article  Google Scholar 

  38. Bitter, G.A., Chen, K.K., Banks, A.R. and Lai, P. (1984) Secretion of Foreign Proteins from Saccharomyces cerevisiae Directed by a-factor Gene Fusion, Proc. Natl. Acad. Sci. USA, 81, 5330–5334

    Article  Google Scholar 

  39. Brake, A.J., Merry Weather, J.F., Coit, D.G., Heberlein, U.A., Masiarz, F.R., Mullenback, G.T., Urdea, M.S., Valenzuela, P. and Barr, P.J. (1984) α -Factor-directed Synthesis and Secretion of Mature Proteins in Saccharomyces cerevisiae, Proc. Natl. Acad. Sci. USA, 81, 4642–4646

    Article  Google Scholar 

  40. Valenzuela, P., Medina, A., Butler, W.J., Ammerer, G. and Hall, B.D. (1982) Synthesis and Assembly of Hepatitis B Virus Surface Antigen Particles in Yeast, Nature, Lond., 298, 347–350

    Article  Google Scholar 

Download references

Authors
  1. Elliot B. Gingold
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Division of Biological and Environmental Sciences, The Hatfield Polytechnic, PO Box 109, College Lane, Hatfield, Hertfordshire, AL10 9AB, England

    John M. Walker (Principal Lecturer in Biochemistry) (Principal Lecturer in Biochemistry)

  2. Department of Infectious Diseases, Section of Bacteriology, University of Utrecht, PO Box 80.171, 3508 TD, Utrecht, The Netherlands

    Wim Gaastra (Associate Professor of Microbiology) (Associate Professor of Microbiology)

Rights and permissions

Reprints and permissions

Copyright information

© 1987 John M. Walker and Wim Gaastra

About this chapter

Cite this chapter

Gingold, E.B. (1987). Cloning in Yeast. In: Walker, J.M., Gaastra, W. (eds) Techniques in Molecular Biology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9799-5_8

Download citation

  • DOI: https://doi.org/10.1007/978-1-4615-9799-5_8

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-0-7099-3673-2

  • Online ISBN: 978-1-4615-9799-5

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation