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A model of O·2 -generation in the complex III of the electron transport chain

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Book cover Bioenergetics of the Cell: Quantitative Aspects

Part of the book series: Developments in Molecular and Cellular Biochemistry ((DMCB,volume 25))

Abstract

Oxidation of semiquinone by O2 in the Q cycle is known to be one of the sources of Superoxide anion (O·2 -) in aerobic cells. In this paper, such a phenomenon was analyzed using the chemical kinetics model of electron transfer from succinate to Q·--cytochrome c, including coenzyme Q, the complex III non-heme iron protein FeS III and cytochromes b l, b h and c l Electron transfers from QH 2 to FeS III and cytochrome b l were assumed to occur according to direct transfer mechanism (dynamic channelling) involving the formation of FeS red III Q·-and Q·+-cytochrome b l complexes. For oxidation/reduction reactions involving cytochromes b h and b l, the dependence of the equilibrium and elementary rate constants on the membrane potential (Δψ) was taken into consideration. The rate of O·2 + generation was found to increase dramatically with increase in △ψ above the values found in State 3. On the other hand, the rate of cytochrome c reduction decreased sharply at the same values of the membrane potential. This explains experimental data that the O·2 + generation at State 4 appears to be very much faster than at State 3. A mild uncoupling in State 4 can markedly decrease the Superoxide generation due to a decrease in Δψ below the above mentioned critical level. ΔpH appears to be equally effective as Δψ in stimulation of superoxide production which depends, in fact, upon the Δμ(Mol Cell Biochem 184: 21–33, 1998)

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References

  1. Kholodenko BN: Control of molecular transformations in multienzyme systems: quantitative theory of metabolic regulation. Mol Biologiya Russia 22:1238–1256, 1988

    CAS  Google Scholar 

  2. Trumpower BL: The protonmotive Q cycle. J Biol Chem 265:11409–11412, 1990

    PubMed  CAS  Google Scholar 

  3. Skulachev VP: Membrane Bioenergetics. Springer, Berlin, 1988

    Book  Google Scholar 

  4. Kholodenko BN, Westerhoff HV, Cascante M: Channelling and the concentration of bulk phase intermediates as cytosolic proteins get more concentrated. Biochem J 313:921–926, 1996

    PubMed  CAS  Google Scholar 

  5. Grivennikova VG, Vinogradov AD: Kinetics of ubiquinone reduction by the resolved succinate: Ubiquinone reductase. BBA 682:491–495, 1982

    Article  PubMed  CAS  Google Scholar 

  6. Kotlyar AB, Vinogradov AD: Interaction of membrane-bound succinate dehydrogenase with substrate and competitive inhibitors. BBA 784:24–34, 1984

    Article  PubMed  CAS  Google Scholar 

  7. Cramer WA, Knaff DB: Energy Transduction in Biological Membranes. Springer-Verlag, Berlin, 1991

    Google Scholar 

  8. Westerhoff HV, Van Dam K: Thermodynamics and Control of Biological Free-Energy Transduction. Elsevier, Amsterdam, 1987

    Google Scholar 

  9. Rich PR: Electron and proton transfers through quinones and cytochrome bc l complexes. BBA 768:53–79, 1984

    Article  PubMed  CAS  Google Scholar 

  10. Hoffman P, Morgan TV, Der Vartanian DV: Respiratory chain characteristics mutants of Azotobacter vinelandii negative to tetramethyl-p-phenylenediamine oxidase. Eur J Biochem 100:19–27, 1979

    Article  PubMed  CAS  Google Scholar 

  11. Boork J, Wennestrom H: The influence of membrane potentials on reaction rates. Control in free-energy transducing systems. BBA 767:314–320, 1984

    Article  PubMed  CAS  Google Scholar 

  12. Reynolds IA, Johnson EA, Tanford C: Incorporation of membrane potential into theoretical analysis of electrogenic ion pumps. Proc Natl Acad Sci USA 82:6869–6873, 1985

    Article  PubMed  CAS  Google Scholar 

  13. Kacser H, Burns JA: The control of the flux. In: DD Davies (ed). Rate Control of Biological Processes. Cambridge University Press, London, 1972, pp 65–104

    Google Scholar 

  14. Kacser H, Burns JA: Molecular democracy: Who shares the controls? Biochem Soc Trans 7:1149–1160, 1979

    PubMed  CAS  Google Scholar 

  15. Heinrich R, Rapoport TA: A linear steady-state treatment of enzymatic chains. General properties, control and effective strength. Eur J Biochem 42:89–95, 1974

    Article  PubMed  CAS  Google Scholar 

  16. Heinrich R, Rapoport TA: Mathematical analysis of multienzyme systems: II. Steady state and transient control. BioSystems 7:130–136, 1975

    Article  PubMed  CAS  Google Scholar 

  17. Kholodenko BN, Westerhoff HV: Control theory of one enzyme. Biochim Biophys Acta 1208:294–305, 1994

    Article  PubMed  Google Scholar 

  18. Kholodenko BN, Westerhoff HV: Metabolic channelling and control of the flux. FEBS Lett 320:71–74, 1993

    Article  PubMed  CAS  Google Scholar 

  19. Van Dam K, Van der Vlag J, Kholodenko BN, Westerhoff HV: The sum of the flux control coefficients of all enzymes on the flux through a group-transfer pathway can be as high as two. Eur J Biochem 212:791–799, 1993

    Article  PubMed  Google Scholar 

  20. Kholodenko BN, Westerhoff HV: Control theory of group-transfer pathways. Biochim Biophys Acta 1229:256–274, 1995

    Article  Google Scholar 

  21. Kholodenko BN, Westerhoff HV: How to reveal various aspects of regulation in group-transfer pathways. Biochim Biophys Acta 1229:275–289, 1995

    Article  Google Scholar 

  22. Brand MD, Vallis BPS, Kesseler A: The sum of flux control coefficients in the electron-transport chain of mitochondria. Eur J Biochem 226:819–829, 1994

    Article  PubMed  CAS  Google Scholar 

  23. Kholodenko BN, Westerhoff HV: The macro-versus microworld of biochemical regulation and control. Trends Biochem Sci 20:52–54, 1995

    Article  PubMed  CAS  Google Scholar 

  24. Boveris A, Chance B: The mitochondrial generation and hydrogen peroxide. General properties and effect of hyperbaric oxygen. Biochem J 134:707–716, 1973

    PubMed  CAS  Google Scholar 

  25. Skulachev VP: Lowering of intracellular O2 concentration as a special function of respiratory systems of the cells. Biochemistry (Moscow) 59:1910–1912, 1994 (Russian)

    CAS  Google Scholar 

  26. Skulachev VP: Non-phosphorylating respiration as a mechanism to minimize formation of reactive oxygen species in the cell. Mol Biologiya 29:709–715, 1995 (Russian)

    Google Scholar 

  27. Skulachev VP: Role of uncoupled and non-coupled oxidations maintenance of safely low levels of oxygen and its one-electron reductants. Quart Rev Biophys 29:169–202, 1996

    Article  CAS  Google Scholar 

  28. Green DE, Wharton DC: Stoichiometry of the fixed oxidation-reduction components of the electron transfer chain of beef heart mitochondria. Biochem Z 336:335–346, 1963

    Google Scholar 

  29. Srere PA:TIBS 6:4–6, 1981

    Google Scholar 

  30. Jones DP: Intracellular diffusion gradients of O2 and ATP. Am J Physiol 250:C663–C675, 1986

    PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Bioenergetics, A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, 119899, Russia

    O. V. Demin, B. N. Kholodenko & V. P. Skulachev

  2. Department of Microbial Physiology, Free University, De Boelelaan 1087, NL-1081, HV Amsterdam, The Netherlands

    B. N. Kholodenko

Authors
  1. O. V. Demin
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  2. B. N. Kholodenko
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  3. V. P. Skulachev
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Editor information

Editors and Affiliations

  1. Laboratories of Bioenergetics, Institute of Chemical and Biological Physics, Tallinn, Estonia

    Valdur A. Saks

  2. Cellular and Molecular Cardiology, U 446 INSERM, Chatenay-Malabry, France

    Renée Ventura-Clapier

  3. Laboratory of Bioenergetics, Joseph Fourier University, Grenoble, France

    Valdur A. Saks , Xavier Leverve  & André Rossi ,  & 

  4. Laboratory of Bioenergetics, Institute of Biochemistry and Cellular Genetics CNRS, Bordeaux, France

    Michel Rigoulet

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© 1998 Springer Science+Business Media Dordrecht

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Demin, O.V., Kholodenko, B.N., Skulachev, V.P. (1998). A model of O·2 -generation in the complex III of the electron transport chain. In: Saks, V.A., Ventura-Clapier, R., Leverve, X., Rossi, A., Rigoulet, M. (eds) Bioenergetics of the Cell: Quantitative Aspects. Developments in Molecular and Cellular Biochemistry, vol 25. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5653-4_3

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  • DOI: https://doi.org/10.1007/978-1-4615-5653-4_3

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-7587-6

  • Online ISBN: 978-1-4615-5653-4

  • eBook Packages: Springer Book Archive

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