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Bioaugmentation Using Repeated Inoculations of Xenobiotic-Degrading Bacteria

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Novel Approaches for Bioremediation of Organic Pollution

Abstract

Bioaugmentation is a promising technology for the cleanup of contaminated soils (Vogel, 1996), and has been tested for a variety of soil contaminants. Recent studies, some of which have been conducted in the field, include experiments with alachlor (McFarlandet al., 1996), atrazine (Grigg et al., 1997; de Souzaet al., 1997), 2,4-D (Digiovanniet al., 1996), carbon tetrachloride (Mayotteet al., 1996), polycyclic aromatic hydrocarbons (Otte.et al., 1994), pentachlorophenol (Otteet al., 1994; Lamaret al., 1994), and trichloroethylene (Munakata-Marret al., 1996; Dubaet al., 1996). Viewed optimistically, results from laboratory experiments generally suggest that any compound that can be used as a growth substrate or cometabolized by microorganisms can be remediated through bioaugmentation. Unfortunately, in most field studies soil inoculants have proven to be unreliable, especially when soils are inoculated with only a single application of degrader bacteria. Inconsistencies between the laboratory and the field have been attributed to nutrient limitations, suppression of the introduced bacteria by protozoa, metal or chemical toxicities, limited transport of bacteria through the soil, and low bioavailability of the contaminant, all of which lead to poor survival and activity of the inoculum (Alexander, 1994).

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Crowley, D.E., Gilbert, E.S., Singer, A.C., Newcombe, D.A., Yang, CH. (1999). Bioaugmentation Using Repeated Inoculations of Xenobiotic-Degrading Bacteria. In: Fass, R., Flashner, Y., Reuveny, S. (eds) Novel Approaches for Bioremediation of Organic Pollution. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4749-5_26

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  • DOI: https://doi.org/10.1007/978-1-4615-4749-5_26

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-7153-3

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