Abstract
The 16S rRNA encoding gene of the magnetogenic bacterium Aquaspirillum magnetotacticum strain MS1 was amplified by the polymerase chain reaction, cloned and sequenced. The amplified DNA sequence was generated with two eubacterial consensus oligodeoxynucleotide primers defining the majority of the 16S rRNA gene. Sequence analysis of the amplified, cloned DNA indicated that A. magnetotacticum MS1 belongs in the alpha purple eubacterial phylogenetic group. Nucleotide sequence obtained from reverse transcription of 16S rRNA from A. magnetotacticum MS1 was consistent with that of the cloned, amplified DNA confirming that the latter originated from strain MS1. Ribosomal RNA sequence analysis can be expected to define the phylogenetic relatedness among magnetogens.
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Literature cited
Balkwell, D.L., Maratea, D., Blakemore, R.P., 1980. Ultrastructure of a magnetotactic spirillum. J. Bacteriol. 141:1399–1408.
Biggin M.D. Gibson, T.J. Hong, G.F. 1983. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc. Natl. Acad. Sci. USA. 80:3963–3965.
Birnboim, H.C., Doly, J. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7:1513–1523.
Blakemore, R.P., Frankel, R.B., 1989. Biomineralization by magnetogenic bacteria, p. 85–98 In R.K. Poole and G.M. Gadd (ed.) Metal-microbe interactions. IRL Press, New York.
Blakemore, R.P., Maratea, D., Wolfe, R.S. 1979. Isolation and pure culture of a freshwater magnetic spirillum in chemically defined medium. J. Bacteriol. 140:720–729
Davis, L.G., Dibner, M.D., Battey, J.F. 1986. Basic methods in molecular biology. Elsevier, New York, pp. 220–232
Eden, P.A., Schmidt, T.M., Blakemore, R.P., and Pace, N.R. 1990. Phylogenetic analysis of Aquaspirillum magnetotacticum using PCR-amplified 16S ribosomal DNA. A.E.M. Submitted for publication.
Frankel, R.B., Blakemore, R.P., 1980. Navigational compass in magnetic bacteria. J. Magnetism and magnetic materials. 15–18:1562–1564.
Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J. MoLBiol. 166:557–580
Lane D.L., Pace, B., Olsen, G.J., Stahl, D.A., Sogin, M.L., and Pace, N.R. 1985. Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc. Natl. Acad. Sci. 82:6955–6959.
Lovely, D.R., Stolz, J.F., Nord, G.L. Jr, Phillips, E.J.P. 1987. Anaerobic production of magnetite by a dissimilatory iron-reducing microorganism. Nature 330:252–254
Maniatis, T., Fritsch, E.F., Sambrook, J. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp. 1–435.
Olsen, G.J. 1987. The earliest phylogenetic branchings: comparing rRNA-based evolutionary trees inferred with various techniques. Cold Spring Harbor Symp. Quant. Biol. 52:825–838.
Olsen, J., Lane, D., Giovannoni, S.J., Pace, N.R., 1986. Microbial ecology and evolution: a ribosomal RNA approach. Ann. Rev. Microbiol. 40:337–365
Saiki, R.K., Gelfand, D.H., Stoffel, S., Scharf, S.J., Higuchi, R., Horn, G.T., Mullis, K.B., and Erlich, H.A. 1988. Primer-directed enzymatic amplification of DNA with a thermostable DBNA polymerase. Science. 239:487–491.
Williams, R.J.P. 1990. Iron and the origin of life. Nature, 343:213–214.
Woese, C.R. 1987. Bacterial evolution. Microbiol. Rev. 51:221–271.
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Eden, P.A., Schmidt, T.M., Blakemore, R.P., Pace, N.R. (1991). Phylogenetic Analysis of Aquaspirillum Magnetotacticum Using PCR-Amplified 16S Ribosomal RNA-Specific DNA. In: Frankel, R.B., Blakemore, R.P. (eds) Iron Biominerals. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3810-3_9
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DOI: https://doi.org/10.1007/978-1-4615-3810-3_9
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