Abstract
In animal tissues exposed to ethanol, the formation of an abnormal phospholipid, phosphatidylethanol (PEth), has been demonstrated (Alling et al., 1983; Alling et al., 1984). This phospholipid is formed through a transphosphatidylation reaction mediated by phospholipase D (Gustavsson and Alling, 1987; Kobayashi and Kanfer, 1987). The ability to activate phospholipase D and thus stimulate phosphatidylethanol formation if ethanol is present has been ascribed to several receptor agonists and protein kinase C activators (Exton, 1990; Billah and Anthes, 1990; Shukla and Halenda, 1991). Thus considerable quantities of phosphatidylethanol may be formed in ethanol-exposed tissues, including brain. Some recent studies have, in addition, suggested that PEth is degraded only slowly (Metz and Dunlop, 1991; Gustavsson et al, 1991). Therefore significant amounts of PEth may accumulate during a period of ethanol exposure.
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References
Alling C., Gustavsson L. and Änggård E. (1983) An abnormal phospholipid in rat organs after ethanol treatment. FEBS Lett. 152, 24–28.
Alling C., Gustavsson L., Månsson J-E., Benthin G. and Änggård E. (1984) Phosphatidylethanol formation in rat organs after ethanol treatment. Biochim.Biophys.Acta 793, 119–122.
Asaoka Y., Kikkawa U., Sekiguchi K., et al. (1988) Activation of a brain-specific protein kinase C subspecies in the presence of phosphatidylethanol. FEBS Lett. 231, 221–224.
Asaoka Y. (1989) Distinct effects of phosphatidylethanol on three types of rat brain protein kinase C Kobe J. Med. Sci. 35, p. 229–237.
Berridge M.J. (1984) Inositol trisphosphate and diacylglycerol as second messengers. Biochem.J. 220, 345–360.
Berridge M.J. and Irvine R.F. (1984) Inositol trisphosphate, a novel second messenger in cellular signal transduction. Nature 312, 315–321.
Billah M.M. and Anthes J.C. (1990) The regulation and cellular functions of phosphatidylcholine hydrolysis. Biochem. J. 269, 281–291.
Bondeson J. and Sundler R. (1987) Phosphatidylethanol counteracts calcium-induced membrane fusion but promotes proton-induced fusion. Biochim.Biophys Acta 899, 258–264.
Bradford M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal.Biochem. 72, 248–254.
Bredt D.S., Mourey R.J. and Snyder S.H. (1989) A simple, sensitive, and specific radioreceptor assay for inositol 1,4,5-trisphosphate in biological tissues. Biochem.Biophys. Res. Commun. 159, 976–982.
Chakravarthy B.R., Bussey A., Whitfield J.F., Sikorska M., Williams R.E. and Durkin J.P. (1991) The direct measurement of protein kinase C (PKC) activity in isolated membranes using a selective peptide substrate. Anl. Biochem. 196, p. 144–150.
Costa L.G. (1990) The phosphoinositide protein kinase C system as a potential target for neurotoxicity. Pharm.Res. 22, 393–408.
Diamond I. (1989) Alcoholic myopathy and cardiomyopathy. N.Engl.J.Med. 320, 458–459.
Eibl H. and Kovatchev S. (1981) Preparation of phospholipids and their analogs by phospholipase D. Methods Enzymol. 72, 632–639.
Exton J.H. (1990) Hormonal regulation of phosphatidylcholine breakdown. Biology and Medicine of Signal Transduction 24, 152–157.
Gustavsson L. and Alling C (1987) Formation of phosphatidylethanol in rat brain by phospholipase D. Biochem.Biophys.Res. Commun. 142, 958–963.
Gustavsson L., Moehren G. and Hoek J.B. (1991) Phosphatidylethanol formation in rat hepatocytes. Ann.N.Y.Acad.Sciences 625, 438–440.
Higashi K. and Hoek J.B. (1991) Ethanol causes desensitization of receptor-mediated phospholipase C activation in isolated hepatocytes. J.Biol.Chem. 266, 2178–2190.
Kobayashi M. and Kanfer J.N. (1987) Phosphatidylethanol formation via transphosphatidylation by rat brain syaptosomal phospholipase D. J.Neurochem. 48, 1597–1603.
Lundqvist C., Rodriguez F.D., Simonsson P., Alling C. and Gustavsson L. (1993)
Phosphatidylethanol affects inositol 1, 4, 5-trisphosphate levels in NG108-15 neuroblastoma x glioma hybrid cells. J.Neurochem. 60, 738–744.
Messing R.O., Peterson P.J., and Henrich C.J. (1991) Chronic ethanol exposure increases levels of protein kinase-C-delta and kinase-C-epsilon and protein kinase-C-mediated phosphorylation in cultured neural cells. J.Biol.Chem. 266, 23428–23432.
Metz S.A. and Dunlop M. (1991) Inhibition of the metabolism of phosphatidylethanol and phosphatidic acid, and stimulation of insulin release, by propranolol in intact pancreatic islets. Biochem. Pharmacol. 41, R1 - R4.
Omodeo-Salé F., Lindi C., Palestini P. and Masserini M. (1991) Role of phosphatidylethanol in membranes - effects on membrane fluidity, tolerance to ethanol, and activity of membrane-bound enzymes. Biochemistry. 30, 2477–2482.
Shukla S.D. and Halenda S.P. (1991) Phospholipase D in cell signalling and its relationship to phospholipase C. Life Sci. 48, 851–866.
Urbano-Marquez A., Estruch R., Navarro-Lopez F., Grau J.M., Mont L. and Rubin E. (1989) The effects of alcoholism on skeletal and cardiac muscle. N.Engl.J.Med. 320, 409–415.
Yasuda I., Kishimoto A., Tanaka S-I., Tominaga M., Sakurai A., Nishizuka Y. (1990) A synthetic peptide substrate for selective assay of protein kinase C. Biochem.Biophys.Res. Comm. 166, 1220–1227.
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Lundqvist, C., Larsson, C., Alling, C., Gustavsson, L. (1993). Phosphatidylethanol Effects on Inositol 1,4,5-Trisphosphate Levels and Protein Kinase C Activity in NG108-15 Cells. In: Alling, C., Diamond, I., Leslie, S.W., Sun, G.Y., Wood, W.G. (eds) Alcohol, Cell Membranes, and Signal Transduction in Brain. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2470-0_7
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DOI: https://doi.org/10.1007/978-1-4615-2470-0_7
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