Abstract
The importance of the aflatoxins from food safety and economic standpoints has continued to drive the development of new analytical methods for these mycotoxins. Currently the widely used methods for measurement of aflatoxins fall into two groups, the established chromatographic methods and traditional enzyme-linked immunosorbent assays (ELISAs). Recently substantial progress has been made in the application of new technologies to the monitoring of aflatoxins. In particular, several research groups have developed biosensors for detection of the toxins as well as presumptive tests for fungal infection. Biosensors have been developed in a variety of formats including surface plasmon resonance, fiber optic probes, and microbead-based assays. The sensitivity and selectivity of the biosensors and of the presumptive tests has reached the level the where the application of these techniques to the screening of foods warrants further investigation.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Anderson, G.P., Breslin, K.A., and Ligler, F.S., 1996, Assay development for a portable fiberoptic biosensor, ASAIO J. 942.
Andreou, V., and Nikolelis, D., 1998, Flow injection monitoring of aflatoxin M, in milk and milk preparations using filter-supported bilayer lipid membranes, Anal. Chem. 70:2366.
Boiarski, A.A., Busch, J.R., Brody, R.S., Ridgway, R.W., Altman, W.P., and Golden, C., 1996, Integrated optic sensor for measuring aflatoxin-B, in com, SPIE 2686:45.
Carlson, M.A., Bargeron, C.B., Benson, R.C., Fraser, A.B., Phillips, T.E., Velky, J.T., Groopman, J.D., Strickland, P.T., and Ko, H.W., 2000, An automated, handheld biosensor for aflatoxin, Biosen. Bioelect. 14:841.
Carter, R.M., Jacobs, M.B., Lubrano, G.J., and Guilbault, G.G., 1997, Rapid detection of aflatoxin B1 with inununochemical optrodes, Anal. Lett. 30:1465.
Checovich, W.J., Bolger, R.E., and Burke, T., 1995, Fluorescence polarization-a new tool for cell and molecular biology, Nature 375:254.
Chu, F.S., 1996, Recent studies on immunoassays for mycotoxins, in: Immunoassays for Residue Analysis, R.C. Beier and L.H. Stanker, eds., American Chemical Society, Washington, DC.
Dandliker, W.B., and Feigen, G.A., 1961, Quantitation of the antigen-antibody reaction by the polarization of fluorescence, Biochem. Biophy. Res. Commun. 5:299.
Dickens, J.W., and Whitaker, T.B., 1981, Bright greenish-yellow fluorescence and aflatoxin in recently harvested yellow corn marketed in North Carolina, J. Amer. Oil Chem. Soc. 58:973A.
Gordon, S.H., Jones, R.W., McClelland, J.F., Wicklow, D.T., and Greene, R.V., 1999, Transient infrared spectroscopy for detection of toxigenic fungi in corn: potential for on-line evaluation, J Agric. Food Chem. 47:5267.
Gordon, S.H., Schudy, R.B., Wheeler, B.C., Wicklow, D.T., and Greene, R.V., 1997, Identification of Fourier transform infrared photoacoustic spectral features for detection of Aspergillusflavus infection in corn, Intl. J. Food Microbiol. 35:179.
Greene, R.V., Gordon, S.H., Jackson, M.A., Bennett, G.A., McClelland, J.F., and Jones, R.W., 1992, Detection of fungal contamination in corn: potential of FTIR-PAS and -DRS, J. Agric. Food Chem. 40:1144.
Haber, E., and Bennett, J.C., 1962, Polarization of fluorescence as a measure of antigen-antibody interaction, Proc. Natl. Acad. Sci. USA. 48:1935.
Hirano, S., Okawara, N., and Narazki, S., 1998, Near infra red detection of internally moldy nuts, Biosci. Biotechnol. Biochem. 62:102.
Ho, J.A., and Durst, R.A., 2000, Development of a flow-injection liposome immunoanalysis system for fumonisin B1, Anal. Chim. Acta 414:61.
Holcomb, M., Wilson, D.W., Trucksess, M.W., and Thompson, H.C., 1992, Determination of aflatoxin in food products by chromatography, J. Chrom. 624:341.
Horwitz, W., Albert, R., and Nesheim, S., 1993, Reliability of mycotoxin assays-an update. J. AOACInt1. 76:461.
Ligler, F.S., Bredehorst, R., Talebian, A., Shriver, L.C., Hammer, C.F., Sheridan, J.P., Vogel, C.W., and Gaber, B.P., 1987, A homogeneous immunoassay for the mycotoxin T-2 utilizing liposomes, monoclonal antibodies, and complement, Anal. Biochem. 163:369.
Maragos C.M., 1997, Detection of the mycotoxin fumonisin B1 by a combination of immunofluorescence and capillary electrophoresis, Food Agric. Immunol. 9:147.
Maragos, C.M., and Thompson, V.S.,2000,Fiber-optic immunosensor for mycotoxins, Natural Toxins 7:(in press).
McClure, W.F., and Farsaie, A.,1980, Dual-wavelength fiber optic photometer measures fluorescence of aflatoxin contaminated pistachio nuts, Trans. ASAE, 204.
Mullett, W., Lai, E.P.C., and Yeung, J.M., 1998, Immunoassay of fumonisins by a surface plasmon resonance biosensor, Anal. Biochem., 258:161.
Nasir, M.S., and Jolley, M.E., 1999, Fluorescence polarization: an analytical tool for immunoassay and drug discovery, Combinatorial Chemistry & High Throughput Screening, 2:177.
Pearson, T.C., and Schatzki, T.F., 1998, Machine vision system for automated detection of aflatoxincontaminated pistachios, J. Agric. Food Chem. 46:2248.
Pearson, T., 1996, Machine vision system for automated detection of stained pistachio nuts, Lebensm. Technol. 29:203.
Pestka, J.J., 1994, Application of immunology to the analysis and toxicity assessment of mycotoxins, Food Agric. Immunol. 6:219.
Ruan, R., Ning, S., Song, A., Ning, A., Jones, R., and Chen, P., 1998, Estimation of Fusarium scab in wheat using machine vision and a neural network, Cereal Chem. 75:455.
Scheper, T., Müller, C., Anders, K.D., Eberhardt, F., Plötz, F., Schelp, C., Thordsen, O., and Schügerl, K., 1994, Optical sensors for biotechnological applications, Biosen. Bioelect. 9: 73.
Scott, P.M., and Trucksess, M.W., 1997, Application of immunoaffinity columns to mycotoxin analysis, J. AOAC Intl. 80:941.
Shotwell, O.L., Goulden, M.L., and Hesseltine, C.W., 1972, Aflatoxin contamination: association with foreign material and characteristic fluorescence in damaged corn kernels, Cereal Chem. 49:458.
Shotwell, O.L. and Hesseltine, C.W., 1981, Use of bright greenish yellow fluorescence as a presumptive test for aflatoxin in com, Cereal Chem. 58:124.
Siontorou, C.G., Nikolelis, D.P., Miernik, A., and Krull, U.J., 1998, Rapid methods for detection of aflatoxin M, based on electrochemical transduction by self-assembled metal-supported bilayer lipid membranes (s-BLMs) and on interferences with transduction of DNA hybridization, Electrochimca Acta, 43:3611.
Strachan, N.J.C., John, P.G., and Miller, LG., 1997, Application of an automated particle-based immunosensor for the detection of aflatoxin B1 in foods, Food Agric. Immunol. 9:177.
Sydenham, E.W., and Shephard, G.S., 1997,Chromatographic and allied methods of analysis for selected mycotoxins, in: Progress in Food Contaminant Analysis, J. Gilbert, ed., Chapman and Hall, New York.
Thompson, V.S., and Maragos, C.M., 1996, Fiber-optic immunosensor for the detection of fumonisin B1, J. Agric. Food Chem., 44:1041.
Trucksess, M.W., and Wood, G.E., 1997, Immunochemical methods for mycotoxins in foods, Food Test. Anal. 3:24.
Van der Gaag, B., Burggaaf, R.A., and Wahlström, L., 1996, Application and development of a BIAcore7 for the detection of mycotoxins in food and feed. Poster Presentation at the 110`“ AOAC International Annual Meeting and Exposition, Orlando, Fl. Sept. 8–12.
Van der Gaag, B., Stigter, E., and Pohl, S., 1999, Development of a biosensor for the detection of mycotoxins. Satellite workshop to the Gordon Conference on mycotoxins and phycotoxins, Salsbury Cove, ME, June 17–19.
Van der Gaag, B., and Stigter, E., 1997, Application development on the BlAcore for the detection of mycotoxins in food and feed. Poster Presentation at the 111`s AOAC International Annual Meeting and Exposition, San Diego, CA. Sept 7–11.
Zeringue, H.J. Jr., and Shih, B.Y., 1998, Extraction and separation of the bright-greenish yellow fluorescent material from aflatoxigenic Aspergillus spp. Infected cotton lint by HPLC- UV/FL, J Agric. Food Chem. 46:1071
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2002 Springer Science+Business Media New York
About this chapter
Cite this chapter
Maragos, C.M. (2002). Novel Assays and Sensor Platforms for the Detection of Aflatoxins. In: DeVries, J.W., Trucksess, M.W., Jackson, L.S. (eds) Mycotoxins and Food Safety. Advances in Experimental Medicine and Biology, vol 504. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0629-4_9
Download citation
DOI: https://doi.org/10.1007/978-1-4615-0629-4_9
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-5166-5
Online ISBN: 978-1-4615-0629-4
eBook Packages: Springer Book Archive