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Oxidative Defenses in the Sea Bass, Dicentrarchus Labrax

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Oxygen Transport to Tissue XXIV

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 530))

Abstract

A study on the oxidative defenses during larval growth and under stressconditions was carried out in the bred sea bass, Dicentrarchus labrax. A high-pressure liquid chromatography (HPLC) method was used for the quantitative determination of vitamins C and E; glutathione peroxidase activity was measured by an enzymatic assay. Vitamin E was measured in the seminal fluid, eggs, embryos and larvae. Vitamins C and E, and glutathione peroxidase activity were measured in adults of Dicentrarchus labrax under normal conditions and subjected to hypoxia. Vitamin E content was high in seminal fluid, eggs, and embryos and at the early stage of larval development. It decreased slowly, but steadily, throughout the larval growth. In adults exposed to hypoxia, vitamins C and E levels were significantly lower with respect to the control group. Glutathione peroxidase levels showed a decrease in the hypoxia-subjected group, although the values were not significant.

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References

  1. Halliwell B, Gutteridge JMC. The antioxidant of human extracellular fluids. Arch Biochem Biophys 1990;280:1–8.

    Article  PubMed  CAS  Google Scholar 

  2. Sies H. Strategies of antioxidant defense. Eur J Biochem 1993; 215:213–219.

    Article  PubMed  CAS  Google Scholar 

  3. Halliwell B. Free radicals and antioxidants: A personal view. Nutrition Reviews 1994;52:253–265.

    Article  PubMed  CAS  Google Scholar 

  4. Halliwell B., Murcia MA, Chirico S, Aruoma O I. Free radicals and antioxidants in food and in vivo: what they do and how they work. Critical Review in Food Science and Nutrition 1995;35:7–20.

    Article  CAS  Google Scholar 

  5. Riley JCN, Behrman HR. Minireview oxygen radicals and reactive oxygen species in reproduction. Proc Soc Exp Biol Med 1991;198:781–791.

    PubMed  CAS  Google Scholar 

  6. Aten RF, Duarte KM, Behrman HR. Regulation of ovarian antioxidant vitamins, reduced glutathione, and lipid peroxidation by luteinizing hormone and prostaglandin f-2a. Biol Reprod 1992;46: 401–407.

    Article  PubMed  CAS  Google Scholar 

  7. Aitken JR, Clarkson JS, Fishel S. Generation of oxygen species, lipid peroxidation, and human sperm function. Biol. Reprod.1989;40: 183–197.

    Article  Google Scholar 

  8. Nars-Esfahani MH, Johnson MH. Quantitative analysis of cellular glutathione in early preimplantation mouse embryos developing in vivo and in vitro Human Reproduction 1992;7:1281–1290.

    Google Scholar 

  9. Goodman-Gilmann A, Theodore WR, Nies AS, Almer T. In: Zanichelli, editor. Le basi farmacologiche della terapia: Vitamina E. Bologna,1992;1460–1464.

    Google Scholar 

  10. Baker GHW, Brindle J, Irvine SD, Aitken JR. Protective effect of antioxidant on the impairment of sperm motility by activated polimorphonuclear leukocytes. Fertility and Sterility 1996;65:411–419.

    PubMed  CAS  Google Scholar 

  11. Wilhelm F.D, Boveris A. Antioxidant defences in marine fish II. Elasmobranchs. Comp Biochem Physiol 1993;106C:415–418.

    Google Scholar 

  12. Wilhelm F.D, Giulivo C., Boveris A. Antioxidant defences in marine fish I. Teleosts. Comp Biochem Physiol 1993;65C:409–413.

    Google Scholar 

  13. Rudneva T. Change in the activity of antioxidant enzymes in the processes of early ontogenesis of various species of black sea fishes. UK Biokhi Zh 1995; 67:92–95.

    Google Scholar 

  14. Umaoka N. Effects of oxygen toxicity on early development of mouse embryo. Molec Reprod Develop 1992;31:28–33.

    Article  CAS  Google Scholar 

  15. Surai PF, Noble RC, Speake BK. Tissue-specific differences in antioxidant distribution and susceptibility to lipid peroxidation during development of the chick embryo. Biochim Biophys Acta 1996; 1304:1–10.

    Google Scholar 

  16. Aceto A, Amicarelli F, Sacchetta P, Dragani B, Bucciarelli T, Masciocco L, Miranda M, Di Ilio C. Developmental aspects of detoxifying enzymes in fish (Salmo iridalus). Free Rad Biol Med 1994;21:285–294.

    CAS  Google Scholar 

  17. Saroglia M, Ingle E, Del Prete M, z L, Venzi L. Tecniche di acquacoltura. Le tecnologie di allevamento. Saroglia M, Ingle E, editors. Edagricole l992;7l-162.

    Google Scholar 

  18. Huo, JZ, Nelis, HJ, Lavens, P.; Sorgeloos, P, De Leenheer, AP. Determination of vitamin E in aquatic organisms by high performance liquid chromatography with fluorescence detection. Analytical Biochem 1996;242:123–128.

    CAS  Google Scholar 

  19. Passey RB, Bee DE, Caffo A, Erikson JM. Evaluation of the linearity of quantitative analytical methods. NCCLS Document EP6-P, 6:8–11.

    Google Scholar 

  20. Akshes A, LR. Naja. Catalase, glutathione peroxidase and superoxide dismutase in different fish species. Comp Biochem Physiol 1981;69B:893–896.

    Google Scholar 

  21. Umaoka N. Effects of oxygen toxicity on early development of mouse embryo. Molec Reprod Develop 1992;31:28–33.

    Article  CAS  Google Scholar 

  22. Mani-Ponset, Guyot, E, Draw JP, Connes R. Utilisation of yolk reserves during post-embryonic development in three teleosts an species: the sea bream Sparus aurata, the sea bass Dicentrarchus labrax and the pike-perch Stizostedion lucioperca. Marine Biology 1996;126:539–547.

    Article  CAS  Google Scholar 

  23. Meydani M, Evans W, Handelman G, Fielding RA, Meydani SN, Fiatarone MA, Blumberg JB, Cannon JG. Antioxidant response to exercise-induced oxidative stress and protection by vitamin E. Ann NY Acad Sci 1992;30:363–364.

    Article  Google Scholar 

  24. Sharma MK, Buettner GR. Interaction of vitamin C and vitamin E during free radical stress in plasma: an ASR study. Free Rad Biol Med 1993;14:649–653.

    CAS  Google Scholar 

  25. Hamre K, Waagbo R, Berge RK, Oyvind L. Vitamins C and E interact in Juvenile atlantic salmon. Free Rad Biol Med 1997;22:137–149.

    CAS  Google Scholar 

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Guerriero, G., Di Finizio, A., Ciarcia, G. (2003). Oxidative Defenses in the Sea Bass, Dicentrarchus Labrax . In: Dunn, J.F., Swartz, H.M. (eds) Oxygen Transport to Tissue XXIV. Advances in Experimental Medicine and Biology, vol 530. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0075-9_68

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  • DOI: https://doi.org/10.1007/978-1-4615-0075-9_68

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-4912-9

  • Online ISBN: 978-1-4615-0075-9

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