Abstract
The morphological description of the hemopoietic stem cell [the colony forming unit-spleen (CFU-s) cell] is made more difficult by the low incidence of this cell in hemopoietic tissue. An unequivocal description is only achieved if it is followed by proof that the cell described can regenerate the hemopoietic organs of irradiated mice (CFU-s assay). Therefore, maintenance of cell viability is essential. This precludes the use of the microscope as the sole instrument of investigation.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Barr, R. D., Whang-Peng, J., and Perry, S. Hemopoietic stem cells in human peripheral blood. Science, 190: 284, 1975.
Basch, R. S., Janossy, G., and Greaves, M. F. Murine pluripotential stem cells lack la antigen. Nature, 270: 520, 1977.
Brunsting, A., and Mullaney, P. F. Differential light scattering from spherical mammalian cells. Biophys. J., 74: 439, 1974.
Fathman, C. G., Small, M., Herzenberg, L. A., and Weissman, I. L. Thymus cell maturation. II. Differentiation of three “mature” subclasses in vivo. Cell. Immunol., 75: 109, 1975.
Herzenberg, L. A., Sweet, R. G., and Herzenberg, L. A. Fluorescence-activated cell sorting. Scientific American, 234, 5: 108, 1976.
Jovin, T. M., Morris, S. J., Striker, G., Schultens, H. A., Digweed, M., and Arndt-Jovin, D. J. Automatic sizing and separation of particles by ratios of light scattering intensities. J. Histochem. Cytochem., 24: 269, 1976.
Meyer, R. A., and Brunsting, A. Light scattering from nucleated biological cells. Biophys. J., 75: 191, 1975.
Mullaney, P. F., and Dean, P. N. The small angle light scattering of biological cells. Theoretical considerations. Biophys. J., 10: 164, 1970.
Mullaney, P. F. Crowell, J. M., Salzman, G. C. Martin, J. C., Hiebert, R. D., and Good, C. A. Pulse- height light scatter distributions using flow-systems instrumentation. J. Histochem. Cytochem., 24: 298, 1976.
Rubinstein, A. S., and Trobaugh, F. E. Ultrastructure of presumptive hematopoietic stem cells. Blood, 42: 61, 1973.
Russell, J. L., and Van den Engh, G. J. The expression of histocompatibility-2 antigens on haemopoietic stem cells. Tissue Antigens (in press).
Salzman, G. C., Crowell, J. M., Martin, J. C., Trujillo, T. T., Romero, A., Mullaney, P. F., and LaBauve, P. M. Cell classification by laser light scattering: Identification of unstained leukocytes. Acta Cytologica, 19: 374, 1975.
Till, J. E., and McCulloch, E. A. A direct measurement of the radiation sensitivity of normal mouse bone marrow cells. Radiat. Res., 14: 213, 1961.
Van Bekkum, D. W., Van Noord, M. J., Maat, B., and Dicke, K. A. Attempts at identification of hemopoietic stem cell in mouse. Blood, 38: 541, 1971.
Van den Engh, G. J., and Platenburg, M. Suppression of CFU-s activity by rabbit-anti-mouse brain serum can be overcome by treatment with papain. Exp. Hemat., 6: 627, 1978.
Visser, J., Van den Engh, G., Williams, N., and Mulder, D. Physical separation of the cycling and non- cycling compartments of murine hemopoietic stem cells. In Baum, S. J., and Ledney, G. D., eds., Experimental hematology today, Springer-Verlag, New York, 1977.
Visser, J. W. M., Cram, L. S., Martin, J. C., Salzman, G. C., and Price, B. J. Sorting of murine granulocytic progenitor cells by use of laser light scattering measurements. In A. Desser, et al., eds., Pulse Cytophotometry. Ghent: European Press Medikon, 1978.
Visser, J., Haaijman, J., and Trask, B. Quantitative immunofluorescence in flow cytometry. In Knapp, et al., eds. Immunofluorescence and related staining techniques. Amsterdam: Elsevier/North Holland, 1978.
Visser, J., van den Engh, G., Williams, N., and Mulder, D. Buoyant densities and sedimentation rates of the murine haemopoietic pluripotent stem cells. In Bentvelzen, P. et al., eds. Advances in comparative leukemia research, 1977. Amsterdan: Elsevier/North Holland, 1978.
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1979 Springer-Verlag New York Inc.
About this paper
Cite this paper
van den Engh, G., Visser, J., Trask, B. (1979). Identification of CFU-s by Scatter Measurements on a Light Activated Cell Sorter. In: Baum, S.J., Ledney, G.D. (eds) Experimental Hematology Today 1979. Experimental Hematology Today, vol 1979. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-6179-7_3
Download citation
DOI: https://doi.org/10.1007/978-1-4612-6179-7_3
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4612-6181-0
Online ISBN: 978-1-4612-6179-7
eBook Packages: Springer Book Archive