Skip to main content
SpringerLink
Log in

Cellulase Adsorption and an Evaluation of Enzyme Recycle During Hydrolysis of Steam-Exploded Softwood Residues

  • Chapter
Biotechnology for Fuels and Chemicals

Part of the book series: Applied Biochemistry and Biotechnology ((ABAB))

Abstract

The sugar yield and enzyme adsorption profile obtained during the hydrolysis of SO2-catalyzed steam-exploded Douglas-fir and posttreated steam-exploded Douglas-fir substrates were determined. After hot alkali peroxide posttreatment, the rates and yield of hydrolysis attained from the posttreated Douglas-fir were significantly higher, even at lower enzyme loadings, than those obtained with the corresponding steam-exploded Douglas-fir. The enzymatic adsorption profiles observed during hydrolysis of the two substrates were significantly different. Ultrafiltration was employed to recover enzyme in solution (supernatant) and reused in subsequent hydrolysis reactions with added, fresh substrate. These recycle findings suggested that the enzyme remained relatively active for three rounds of recycle. It is likely that enzyme recovery and reuse during the hydrolysis of posttreated softwood substrates could lead to reductions in the need for the addition of fresh enzyme during softwood-based bioconversion processes.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Gregg, D. J. and Saddler, J. N. (1996), Biotechnol. Bioeng. 51, 375–383.

    Article  PubMed  CAS  Google Scholar 

  2. Katzen, R. and Fowler, D. (1994), Appl. Biochem. Biotechnol. 45, 697–707.

    Article  PubMed  Google Scholar 

  3. Lee, D., Yu, A. H. C., and Saddler, J. N. (1995), Biotechnol. Bioeng. 45, 328–336.

    Article  PubMed  CAS  Google Scholar 

  4. Tan, L. U. L., Yu, E. K. C., Campbell, N., and Saddler, J. N. (1986), Appl. Microbiol. Biotechnol. 25, 250–255.

    CAS  Google Scholar 

  5. Tjerneld, F., Persson, I., and Lee, J. M. (1991), Biotechnol. Bioeng. 37, 876–882.

    Article  PubMed  CAS  Google Scholar 

  6. Yang, B., Lu, Y., Gao, K., and Deng, Z. (1998), Membr. Sci. Technol. 4, 3–10.

    Google Scholar 

  7. Ramos, L. P. and Saddler, J. N. (1994), Appl. Biochem. Biotechnol. 45/46, 193–207.

    Article  Google Scholar 

  8. Oltus, E., Mato, J., Bauer, S., and Farkas, V. (1987), Cellulose Chem. Technol. 21, 663–672.

    CAS  Google Scholar 

  9. Peters, M. S. and Timmerhaus, K. D. (1991), Plant Design and Economics for Chemical Engineers, McGraw-Hill, NY.

    Google Scholar 

  10. Sutcliffe, R. and Saddler, J. N. (1986), Biotechnol. Bioeng. 17, 749–762.

    CAS  Google Scholar 

  11. Ishihara, M., Uemura, S., Hayashi, N., Jellison, J., and Shimizu, K. (1991), J. Ferment. Bioeng. 72, 96–100.

    Article  CAS  Google Scholar 

  12. Lee, D., Yu, A.H.C., Wong, K.K.Y., and Saddler, J.N. (1994), Appl. Biochem. Biotechnol. 45/46, 407–415.

    Article  Google Scholar 

  13. Chernoglazov, V., Ermolova, O., and Klyosov, A. (1988), Enzyme Microb. Technol. 10, 503–507.

    Article  CAS  Google Scholar 

  14. Saddler, J. N. and Mackie, K. (1990), Biomass 22, 293–305.

    Article  Google Scholar 

  15. Mansfield, S. D., Mooney, C., and Saddler, J. N. (1999), Biotechnol. Prog. 15, 804–816.

    Article  PubMed  CAS  Google Scholar 

  16. Yang, B., Boussaid, A., Mansfield, S. D. and Saddler, J.N. (2001), Biotechnol. Bioeng., in press.

    Google Scholar 

  17. Ghose, T. K. (1987), Pure Appl. Chem. 59(2), 257–268

    Article  CAS  Google Scholar 

  18. TAPPI, Technical Association of the Pulp and Paper Industry (1998), TAPPI Standard Methods, T-222 om-98.

    Google Scholar 

  19. TAPPI, Technical Association of the Pulp and Paper Industry (1991), TAPPI Useful Methods, UM-250, Tappi Press, Atlanta, GA, pp. 47–48.

    Google Scholar 

  20. Schwald, W., Smaridge, T., Chan, M., Breuil, C., and Saddler, J. N. (1989), in Enzyme Systems for Lignocellulosic Degradation, Coughlan, M. P., ed., Elsevier, NY, pp. 231–242.

    Google Scholar 

  21. Wu, M., Chang, K., Boussaid, A., Gregg, D. J., Beatson, R., and Saddler, J. N. (1998), Appl. Biochem. Biotechnol. 77–79, 47–54.

    Google Scholar 

  22. Shevchenko, S. M., Beatson, R. P., and Saddler, J. N. (1999), Appl Biochem. Biotechnol. 77–79, 867–876.

    Article  PubMed  Google Scholar 

  23. Boussaid, A. and Saddler, J. N. (1999), Enzyme Microb. Technol. 24, 138–143.

    Article  CAS  Google Scholar 

  24. Hetti-Palonen, M. T. and Linder, M. (1999), Appl. Environ. Microbiol. 65, 5229–5233.

    Google Scholar 

  25. Singh, A., Kumar, P. K. R., and Schuegert, K. (1991), J. Biotechnol. 18, 205–212.

    Article  CAS  Google Scholar 

  26. Yu, A. H. C., Lee, D., and Saddler, J.N. (1995), Biotechnol. Appl. Biochem. 21, 203–216.

    CAS  Google Scholar 

  27. Lee, S. B., Shin, H. S., and Ryu, D. D. Y. (1982), Biotechnol. Bioeng. 24, 2137–2153.

    Article  PubMed  CAS  Google Scholar 

  28. Mooney, C., Mansfield, S. D., Beatson, R. P. and Saddler, J. N. (1999), Enzyme Microb. Technol. 25, 644–650.

    Article  CAS  Google Scholar 

  29. Vallander, L. and Eriksson, L. K.-E. (1991), Biotechnol. Bioeng. 38, 139–144.

    Article  PubMed  CAS  Google Scholar 

  30. Mes-Hartree, M., Hogan, C. M., and Saddler, J. N. (1987), Biotechnol. Bioeng. 30, 558–564.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Forest Products Biotechnology,Department of Wood Science, University of British Columbia, 4030-2424 Main Mall, Vancouver, B.C., V6T 1Z4, Canada

    Yanpin Lu, Bin Yang, David Gregg, John N. Saddler & Shawn D. Mansfield

Authors
  1. Yanpin Lu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Bin Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. David Gregg
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. John N. Saddler
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Shawn D. Mansfield
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. National Renewable Energy Laboratory, USA

    Mark Finkelstein  & James D. McMillan  & 

  2. Oak Ridge National Laboratory, USA

    Brian H. Davison

Rights and permissions

Reprints and permissions

Copyright information

© 2002 Springer Science+Business Media New York

About this chapter

Cite this chapter

Lu, Y., Yang, B., Gregg, D., Saddler, J.N., Mansfield, S.D. (2002). Cellulase Adsorption and an Evaluation of Enzyme Recycle During Hydrolysis of Steam-Exploded Softwood Residues. In: Finkelstein, M., McMillan, J.D., Davison, B.H. (eds) Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-0119-9_52

Download citation

  • DOI: https://doi.org/10.1007/978-1-4612-0119-9_52

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4612-6621-1

  • Online ISBN: 978-1-4612-0119-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation