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Synchronous Fluorescence Spectroscopy and Its Applications in Clinical Analysis and Food Safety Evaluation

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Part of the book series: Reviews in Fluorescence ((RFLU,volume 2010))

Abstract

Synchronous fluorescence spectroscopy (SFS) plays an important role in the simultaneous analysis of multicomponent samples due to the remarkable advantages of spectral simplification, light scattering reduction, and selectivity improvement over conventional fluorescence spectroscopy. The selectivity and resolution are further enhanced by the combination of synchronous fluorescence approaches with other techniques, such as derivative technique, low-temperature technique, and chemometrics. In this chapter, the methodologies of various SFS approaches are compendiously introduced. Representative examples of biological, environmental, and clinical applications of these techniques are briefly summarized. An emphasis is placed on the development of rapid and simple synchronous fluorescence-based approaches for the determination of carcinogenic polycyclic aromatic hydrocarbons in foods and the differential analysis of porphyrins in human body samples.

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References

  1. Miller JN (1984) Recent developments in fluorescence and chemiluminescence analysis. Analyst 109:91–98

    Article  Google Scholar 

  2. Lloyd JBF (1971) Multicomponent analysis by synchronous luminescence spectrometry. Nature 231:64–67

    CAS  Google Scholar 

  3. He LF, Lin DL, Li YQ (2004) Application and development of synchronous fluorescence spectrometry. Prog Chem 16:879–885

    CAS  Google Scholar 

  4. Cai QH, Zou ZX, Li YQ (2007) Simultaneous determination of Sudan II and Sudan III via synchronous fluorescence spectrometry. Chem J Chin Univ 28:1663–1665

    CAS  Google Scholar 

  5. Inman EL Jr, Files LA, Winefordner JD (1986) Theoretical optimization of parameter selection in constant energy synchronous luminescence spectrometry. Anal Chem 58:2156–2160

    Article  PubMed  CAS  Google Scholar 

  6. Inman EL Jr, Winefordner JD (1982) Constant energy synchronous fluorescence for analysis of polynuclear aromatic hydrocarbon mixtures. Anal Chem 54:2018–2022

    Article  CAS  Google Scholar 

  7. Kubic TA, Lasher CM, Dwyer J (1983) Individualization of automobile engine oils. I. The introduction of variable separation synchronous excitation fluorescence to engine oil analysis. J Forensic Sci 28:186–199

    CAS  Google Scholar 

  8. Blanco M, Cerda V, Coello J et al (1996) Spectrofluorometric identification of polycyclic aromatic hydrocarbons at ppb level. Anal Lett 29:1603–1617

    Article  CAS  Google Scholar 

  9. Murillo-Pulgarin JA, Alanon-Molina A (1994) Matrix isopotential synchronous fluorescence. Direct determination of gentisic acid in urine. Anal Chim Acta 296:87–97

    Article  CAS  Google Scholar 

  10. Li YQ, Huang XZ, Xu JG (1999) Synchronous fluorescence spectrometric methodology in the wavelength domain. J Fluoresc 9:173–179

    Article  CAS  Google Scholar 

  11. Li YQ, Qian F, Li Z (2000) Reduction of second-order scattering interference by variable-angle synchronous luminescence spectroscopy. Chem J Internet 2:30

    Google Scholar 

  12. Weiner ER (1978) Equivalence of simultaneous scanning and three-dimensional plotting of fluorescence spectra. Anal Chem 50:1583–1584

    Article  CAS  Google Scholar 

  13. Yao MN, Li YQ (2001) Analysis of meso-tetrakis (4-sulfonatophenyl) porphyrin in aqueous solution by constant-wavelength synchronous fluorescence spectroscopy. Chem J Internet 5:65

    Google Scholar 

  14. Lage-Yusty MA, Lopez-Gonzalez J, Simal-Lozano J (2005) Resolution of 13 polycyclic aromatic hydrocarbons by constant-wavelength synchronous spectrofluorometry. Anal Sci 21:1203–1206

    Article  PubMed  CAS  Google Scholar 

  15. Li YQ, Huang XZ, Xu JG, Chen GZ (1992) Synchronous spectrofluorimetry for simultaneous determination of riboflavin and pyridoxine and its application in multivitamin preparations. Acta Pharm Sinica 27:52–56

    CAS  Google Scholar 

  16. Inman EL, Winefordner JD (1982) Constant energy synchronous fluorescence for analysis of polynuclear aromatic hydrocarbon mixtures. Anal Chim Acta 138:245–255

    Article  CAS  Google Scholar 

  17. He LF, Lin DL, Li YQ (2005) Micelle-sensitized constant-energy synchronous fluorescence spectrometry for the simultaneous determination of pyrene, benzo[a]pyrene and perylene. Anal Sci 21:641–645

    Article  PubMed  CAS  Google Scholar 

  18. Li YQ, Huang XZ, Xu JG, Chen GZ (1992) Derivative constant-energy synchronous fluorescence for simultaneous analysis of polynuclear aromatic hydrocarbon mixtures. Anal Chim Acta 256:285–291

    Article  CAS  Google Scholar 

  19. Li YQ, Huang XZ (1989) Constant-energy synchronous luminescence spectrometry. Chin J Anal Chem 17:1154–1158

    CAS  Google Scholar 

  20. He LF, Lin DL, Li YQ (2005) Simultaneous analysis of carbazole, benzo(a)pyrene, perylene and 2,3-benzoanthracene by derivative-constant-energy synchronous fluorescence spectroscopy. Environ Chem 24:89–93

    CAS  Google Scholar 

  21. Li YQ, Huang XZ (1990) Simultaneous determination of carbazole, anthracene and perylene by constant-energy synchronous fluorimetry. Chin J Anal Chem 18:827–831

    CAS  Google Scholar 

  22. Oms MT, Forteza R, Cerda V, Garcia-Sanchez F, Ramos Rubio AL (1990) Simultaneous determination of polycyclic aromatic hydrocarbons by variable angle spectrofluorometry. Int J Environ Anal Chem 42:1–14

    Article  CAS  Google Scholar 

  23. Murillo-Pulgarin JA, Alanon-Molina A, Fernandez Lopez P (1998) Simultaneous determination of atenolol, propranolol, dipyridamole and amiloride by means of non-linear variable-angle synchronous fluorescence spectrometry. Anal Chim Acta 370:9–18

    Article  CAS  Google Scholar 

  24. Murillo-Pulgarin JA, Alanon-Molina A (1994) Matrix isopotential synchronous fluorescence direct determination of gentisic acid in urine. Anal Chim Acta 296:87–97

    Article  CAS  Google Scholar 

  25. Sui W, Wu C, Li YQ, Wen WH (2001) Simultaneous determination of 1-chloroanthracene and 9-bromoanthracene by using matrix isopotential synchronous fluorescence spectrometry combined with derivative technique. Chin J Anal Chem 29:320–322

    Google Scholar 

  26. Patra D (2003) Applications and new developments in fluorescence spectroscopic techniques for the analysis of polycyclic aromatic hydrocarbons. Appl Spectrosc Rev 38:155–185

    Article  CAS  Google Scholar 

  27. Murillo-Pulgarin JA, Alanon-Molina A (1994) Determination of salicylic and gentisic acids in the presence of each other by matrix isopotential synchronous fluorescence spectrometry. Analyst 119:1915–1919

    Article  CAS  Google Scholar 

  28. John P, Soutar I (1976) Identification of crude oils by synchronous excitation spectrofluorimetry. Anal Chem 48:520–524

    Article  CAS  Google Scholar 

  29. Garcia-Sanchez F, Cruces-Blanco C (1986) Determination of the carbamate herbicide propham by synchronous derivative spectrofluorometry following fluorescamine-fluorogenic labeling. Anal Chem 58:73–76

    Article  CAS  Google Scholar 

  30. Garcia-Sanchez F, Marquez-Gomez JC, Hernandez-Lopez M (1987) Fluorometric determination of aluminum and beryllium in mixtures by synchronous derivative spectrometry. Analyst 112:649–652

    Article  CAS  Google Scholar 

  31. Cai SY, You WW, Li YQ (2007) Rapid determination of carbendazim in lentinulaedodes by derivative synchronous fluorescence spectrometry. J Instrum Anal 26:117–119

    CAS  Google Scholar 

  32. Murillo-Pulgarin JA, Alanon-Molina A, Fernandez-Lopez P et al (2007) Direct determination of closely overlapping drug mixtures of diflunisal and salicylic acid in serum by means of derivative matrix isopotential synchronous fluorescence spectrometry. Anal Chim Acta 583:55–62

    Article  PubMed  CAS  Google Scholar 

  33. Li YQ, Sui W, Wu C, Yu LJ (2001) Derivative matrix isopotential synchronous fluorescence spectroscopy for the direct determination of 1-hydroxypyrene as a urinary biomarker of exposure to polycyclic aromatic hydrocarbons. Anal Sci 17:167–170

    Article  PubMed  Google Scholar 

  34. Lin DL, Zou ZX, He LF, Li YQ (2005) Rapid screening method for simultaneous determination of four polycyclic aromatic hydrocarbons in water samples by derivative non-linear variable-angle synchronous muorescence spectrometry. Luminescence 20:292–297

    Article  PubMed  CAS  Google Scholar 

  35. Zhou N, Luo HD, Li N, Jia YZ, Li YQ (2011) Highly sensitive and selective spectroluorimetric approach for the rapid determination of trace benzo[α]pyrene in drinking water and in solutions leached from disposable paper cups. Luminescence 26:35–43

    Google Scholar 

  36. Shpol’skii EV, Il’ina AA, Klimova LA (1952) Fluorescence spectrum of coronene in frozen solutions. Dokl Akad Nauk SSSR 87:935–938 (Chem Abstr 45:7882a; 46:8973d; 47:4205b)

    Google Scholar 

  37. Yu LJ, Li YQ, Sui W (2002) Analysis of PAHs by Shpol’ skii fluorescence spectra in low temperature. Spectrosc Spectr Anal 22:819–821

    CAS  Google Scholar 

  38. Inman EL Jr, Winefordner JD (1982) Low-temperature constant-energy synchronous luminescence spectroscopy. Anal Chim Acta 141:241–254

    Article  CAS  Google Scholar 

  39. Zhang W, Lin DL, Zou ZX, Li YQ (2007) A novel approach for simultaneous determination of polycyclic aromatic hydrocarbons by Shpol’ skii non-linear variable-angle synchronous fluorescence spectrometry. Talanta 71:1481–1486

    Article  PubMed  CAS  Google Scholar 

  40. Madrakian T, Afkhami A, Mohammadnejad M (2009) Simultaneous spectrofluorimetric determination of levodopa and propranolol in urine using feed-forward neural networks assisted by principal component analysis. Talanta 78:1051–1055

    Article  PubMed  CAS  Google Scholar 

  41. Armas G, Miró M, Estela JM et al (2002) Simultaneous determination of fluorophores with overlapped spectra by sequential injection analysis coupled to variable angle scanning fluorescence spectrometry and multivariate linear regression algorithms. Anal Chim Acta 471:173–186

    Article  Google Scholar 

  42. Berzas-Nevado JJ, Murillo-Pulgarín JA, Amador-Hernández J et al (1998) Simultaneous fluorimetric determination of pyridoxal, pyrioxamine and pyridoxic by partial least squares using non-linear variable angle synchronous spectra. Analyst 123:483–488

    Article  CAS  Google Scholar 

  43. Andrade-Eiroa A, Vázquez-Blanco E, López-Mahía P, Muniategui-Lorenzo S, Prada-Rodríguez D (2000) Resolution of benzo[a]pyrene in complex mixtures of other polycyclic aromatic hydrocarbons. Comparison of two spectrofluorimetric methods applied to water samples. Analyst 125:1321–1326

    Article  PubMed  CAS  Google Scholar 

  44. Divyaa O, Mishra AK (2007) Combining synchronous fluorescence spectroscopy with multivariate methods for the analysis of petrol–kerosene mixtures. Talanta 72:43–48

    Article  Google Scholar 

  45. Bortolato SA, Arancibia JA, Escandar GM (2008) Chemometrics-assisted excitation emission fluorescence spectroscopy on nylon membranes. Simultaneous determination of benzo[a]pyrene and dibenz[a, h]anthracene at parts-per-trillion levels in the presence of the remaining EPA PAH priority pollutants as interferences. Anal Chem 80:8276–8286

    Article  PubMed  CAS  Google Scholar 

  46. Fernández-Sánchez JF, Segura Carretero A, Cruces-Blanco C, Fernández-Gutiérrez A (2004) Highly sensitive and selective fluorescence optosensor to detect and quantify benzo[a]pyrene in water samples. Anal Chim Acta 506:1–7

    Article  Google Scholar 

  47. Hua GX, Killham K, Singleton I (2006) Potential application of synchronous fluorescence spectroscopy to determine benzo[a]pyrene in soil extracts. Environ Pollut 139:272–278

    Article  PubMed  CAS  Google Scholar 

  48. He LF, Lin DL, Li YQ (2004) Derivative constant-energy synchronous fluorescence spectroscopy for rapid analysis of polynuclear aromatic hydrocarbon mixtures. Chin J Appl Chem 21:937–941

    CAS  Google Scholar 

  49. Commission E (2005) Commission Directive 2005/10/EC of 4 February 2005 laying down the sampling methods and the methods of analysis for the official control of the levels of benzo(a)pyrene in foodstuffs. Off J Eur Union L34:15–20

    Google Scholar 

  50. Li YQ, Li N, Lin DL, He LF (2005) Rapid fluorescence screening method for benz(a)pyrene in food. Patent Appl. 2005–10006077.0, CN 100389319C

    Google Scholar 

  51. Determination of benzo[a]pyrene in foods. The national standards of the People’s Republic of China. GB/T 5009.27-2003, pp 217–222

    Google Scholar 

  52. Zhang W, Zhou N, Li N, Xie YS, Luo HD, Li YQ (2009) Simultaneous determination of PAHs in food by low-temperature constant-energy synchronous fluorescence spectroscopy. Spectrosc Spec Anal 29:2806–2809

    CAS  Google Scholar 

  53. Li XY, Lin LR, Li N, Huang W, Liu Q, Li YQ (2011) Rapid simultaneous analysis of Benzo[a]pyrene, Benzo[k]fuoranthene and anthracene in tea by a novel synchronous fluorimetric method. J Agric Food Chem 59:5899–5905

    Google Scholar 

  54. Lin DL, He LF, Li YQ (2004) Rapid and simultaneous determination of coproporphyrin and protoporphyrin in feces by derivative matrix isopotential synchronous fluorescence spectro­metry. Clin Chem 50:1797–1803

    Article  PubMed  CAS  Google Scholar 

  55. Shindi AAF, Zhou PC, Zou ZX, Li YQ (2010) A novel, selective, and rapid fluorimetric method for the simultaneous analysis of coproporphyrin and uroporphyrin in urine. Anal Chim Acta 664:89–94

    Article  PubMed  CAS  Google Scholar 

  56. Zhou PC, Huang W, Zhang RB, Zou ZX, Luo HD, Shindi AAF, Li YQ (2008) A simple and rapid fluorimetric method for simultaneous determination of protoporphyrin IX and zinc protoporphyrin IX in whole blood. Appl Spectrosc 62:1268–1273

    Article  PubMed  CAS  Google Scholar 

  57. Huang W, Zhou PC, Li YQ (2008) Investigation on solvent for fluorescence analysis of porphyrin in blood. Life Sci Instrum 6:22–25

    Google Scholar 

  58. Huang W, Li YN, Liu Q, Wu HL, Li YQ (2009) Analysis of zinc protoporphrin IX (ZnPP) and protoporphyrin IX (PP) in blood by two fluorimetricc methods. Chin J Anal Chem 37: 1142–1146

    CAS  Google Scholar 

  59. Huang W, Liu Q, Zhu EY, Shindi AAF, Li YQ (2010) Rapid simultaneous determination of protoporphyrin IX, uroporphyrin III and coproporphyrin III in human whole blood by non-linear variable-angle synchronous fluorescence technique coupled with partial least squares. Talanta 82(4):1516–1520

    Article  PubMed  CAS  Google Scholar 

  60. Liu Q, Huang W, Shindi AAF, Li YQ. Rapid simultaneous determination of protoporphyrin IX, coproporphyrin III and zinc protoporphyrin IX in ultramicro erythrocytes by derivative matrix isopotential – non-linear variable-angle synchronous fluorescence spectroscopy Unpublished work

    Google Scholar 

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Acknowledgment

We are grateful for the financial support from the National Natural Science Foundation of China (29875023, 20575055, and 20975084), the National Basic Research Program of China (973 Program, 2007CB935600), the Educational Ministry Foundation of China, the Natural Science Foundation of Fujian Province (No. B0410002), the Science and Technology Program of Fujian Province (2009Y0046), and the Science and Technology Project of Xiamen. We thank Yu-Luan Chen for critical reading of this manuscript.

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Correspondence to Yao-Qun Li .

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Li, YQ. et al. (2012). Synchronous Fluorescence Spectroscopy and Its Applications in Clinical Analysis and Food Safety Evaluation. In: Geddes, C. (eds) Reviews in Fluorescence 2010. Reviews in Fluorescence, vol 2010. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-9828-6_5

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