Abstract
Synchronous fluorescence spectroscopy (SFS) plays an important role in the simultaneous analysis of multicomponent samples due to the remarkable advantages of spectral simplification, light scattering reduction, and selectivity improvement over conventional fluorescence spectroscopy. The selectivity and resolution are further enhanced by the combination of synchronous fluorescence approaches with other techniques, such as derivative technique, low-temperature technique, and chemometrics. In this chapter, the methodologies of various SFS approaches are compendiously introduced. Representative examples of biological, environmental, and clinical applications of these techniques are briefly summarized. An emphasis is placed on the development of rapid and simple synchronous fluorescence-based approaches for the determination of carcinogenic polycyclic aromatic hydrocarbons in foods and the differential analysis of porphyrins in human body samples.
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Liu Q, Huang W, Shindi AAF, Li YQ. Rapid simultaneous determination of protoporphyrin IX, coproporphyrin III and zinc protoporphyrin IX in ultramicro erythrocytes by derivative matrix isopotential – non-linear variable-angle synchronous fluorescence spectroscopy Unpublished work
Acknowledgment
We are grateful for the financial support from the National Natural Science Foundation of China (29875023, 20575055, and 20975084), the National Basic Research Program of China (973 Program, 2007CB935600), the Educational Ministry Foundation of China, the Natural Science Foundation of Fujian Province (No. B0410002), the Science and Technology Program of Fujian Province (2009Y0046), and the Science and Technology Project of Xiamen. We thank Yu-Luan Chen for critical reading of this manuscript.
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Li, YQ. et al. (2012). Synchronous Fluorescence Spectroscopy and Its Applications in Clinical Analysis and Food Safety Evaluation. In: Geddes, C. (eds) Reviews in Fluorescence 2010. Reviews in Fluorescence, vol 2010. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-9828-6_5
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DOI: https://doi.org/10.1007/978-1-4419-9828-6_5
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