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Single Molecule Detection with an Aptamer-Integrated Nanopore

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Abstract

Aptamers, also called “synthetic” antibodies, are a broad class of engineered oligonucleotides that can specifically bind targets with high affinity. Upon binding, these powerful molecules can form complex three-dimensional structures and possess sophisticated functions to inhibit pathogen protein, catalyze chemical reactions, controlling gene expression, and regulate cellular functions. These can be therefore potentially applied as tools for exploring biological systems and medical diagnosis. Nanopore detection technology, on the other hand, can “visually” capture the dynamic binding of a single molecule to a ligand in a nanometer-scaled pore through the discrete changes in conductance upon binding. This ability to track single molecule kinetics has made the nanopore a promising single molecule detector. This chapter will be focused on the use of nanopores as a research tool that can be combined with laboratory nanofabrication, bio-friendly surface engineering and site-directed protein engineering to understand aptamer folding process, the interaction between a single aptamer and its target, and to develop aptamer-encoded nanopore sensors for medical and bio-defense detection.

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Acknowledgments

Financial support from NSF CAREER (0546165), National Institutes of Health (GM079613), University of Missouri Research Board and Startup Fund. The author’s work was conducted in a facility constructed with support from Research Facilities Improvement Program Grant C06-RR-016489-01 from the National Center for Research Resources, National Institutes of Health.

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  1. Department of Biological Engineering and Dalton Cardiovascular Research Center, University of Missouri, Columbia, MO, 65211, USA

    Li-Qun Gu

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  1. Li-Qun Gu
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Correspondence to Li-Qun Gu .

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  1. Department of Electrical Engineering, Nanotechnology Research and Teaching Fac, University of Texas at Arlington, S. Cooper Street, Arlington, 76019-0119, Texas, USA

    Samir M. Iqbal

  2. Department of Electrical and Computer En, University of Illinois at Urbana-Champai, N. Wright Street 208, Urbana, 61820, Illinois, USA

    Rashid Bashir

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© 2011 Springer Science+Business Media, LLC

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Gu, LQ. (2011). Single Molecule Detection with an Aptamer-Integrated Nanopore. In: Iqbal, S., Bashir, R. (eds) Nanopores. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-8252-0_3

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  • DOI: https://doi.org/10.1007/978-1-4419-8252-0_3

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  • Online ISBN: 978-1-4419-8252-0

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