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Self-assembled Nanogel Engineering

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Biomedical Applications of Hydrogels Handbook

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Abstract

Functional nanogels have been designed by the self-assembly of various associating polymers. In particular, cholesterol-bearing polysaccharides form physically crosslinked nanogels by self-assembly in water. The nanogels trap proteins mainly by hydrophobic interaction and show chaperon-like activity. They are useful as polymeric nanocarriers especially in protein delivery. Macrogels with well-defined nanostructures were obtained by self-assembly and chemical crosslinking of these nanogels as building blocks.

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References

  1. Nayak S, Lyon LA (2005) Soft nanotechnology with soft nanoparticles. Angew Chem Int Ed 44:7686–7708

    Article  CAS  Google Scholar 

  2. Oh JK, Drumright R, Siegwart DJ et al (2008) The development of microgels/nanogels for drug delivery applications. Prog Polym Sci 33:448–477

    Article  CAS  Google Scholar 

  3. Akiyoshi K, Deguchi S, Moriguchi N et al (1993) Self-aggregates of hydrophobized polysaccharides in water. Formation and characteristics of nanoparticles. Macromolecules 26:3062–3068

    Article  CAS  Google Scholar 

  4. Akiyoshi K, Deguchi S, Tajima H et al (1997) Microscopic structure and thermoresponsiveness of a hydrogels nanoparticle by self-assembly of a hydrophobized polysaccharide. Macromolecules 30:857–861

    Article  CAS  Google Scholar 

  5. Kuroda K, Fujimoto K, Sunamoto J et al (2002) Hierarchical self-assembly of hydrophobically modified pullulan in water: gelsation by networks of nanoparticles. Langmuir 18:3780–3786

    Article  CAS  Google Scholar 

  6. Akiyoshi K, Sunamoto J (1996) Supramolecular assembly of hydrophobized polysaccharide. Supramol Sci 3:157–163

    Article  CAS  Google Scholar 

  7. Morimoto N, Nomura SM, Miyazawa N et al (2006) Nanogel engineered designs for polymeric drug delivery. In: Svenson S (ed) Polymeric drug delivery volume ii – polymeric matrices and drug particle engineering, ACS Symposium Series 924. American Chemical Society, Washington, DC

    Google Scholar 

  8. Akiyama E, Morimoto N, Kujawa P et al (2007) Self-assembled nanogels of cholesteryl-modified polysaccharides: effect of the polysaccharide structure on their association characteristics in the dilute and semi-dilute regimes. Biomacromolecules 8:2366–2373

    Article  CAS  Google Scholar 

  9. Akiyoshi K, Ueminami A, Kurumada S et al (2000) Self-association of cholesteryl-bearing poly(l-lysine) in water and control of its secondary structure by host-guest interaction with cyclodextrin. Macromolecules 33:6752–6756

    Article  CAS  Google Scholar 

  10. Akiyoshi K, Kang E-C, Kurumada S et al (2000) Controlled association of amphiphilic polymers in water: thermosensitive nanoparticles formed by self-assembly of hydrophobically modified pullulans and poly(N-­isopropylacrylamides). Macromolecules 33:3244–3249

    Article  CAS  Google Scholar 

  11. Morimoto N, Winnik FM, Akiyoshi K (2007) Botryoidal assembly of cholesteryl-pullulan/poly(N-­isopropylacrylamide) nanogels. Langmuir 23:217–223

    Article  CAS  Google Scholar 

  12. Morimoto N, Qiu XP, Winnik FM et al (2008) Dual stimuli-responsive nanogels by self-assembly of polysaccharides lightly grafted with thiol-terminated poly(N-isopropylacrylamide) chains. Macromolecules 41:5985–5987

    Article  CAS  Google Scholar 

  13. Morimoto N, Obeid R, Yamane S et al (2009) Composite nanomaterials by self-assembly and controlled crystallization of poly(2-isopropyl-2-oxazoline)-grafted polysaccharide. Soft Matter 5:1597–1600

    Article  CAS  Google Scholar 

  14. Hirakura T, Nomura Y, Aoyama Y et al (2004) Photoresponsive nanogels formed by the self-assembly of spiropyrane-bearing pullulan that act as artificial molecular chaperones. Biomacromolecules 5:1804–1809

    Article  CAS  Google Scholar 

  15. Nishikawa T, Akiyoshi K, Sunamoto J (1994) Supramolecular assembly between nanoparticles of hydrophobized polysaccharide and soluble protein complexation between the self-aggregate of cholesterol-bearing pullulan and alpha-chymotrypsin. Macromolecules 27:7654–7659

    Article  CAS  Google Scholar 

  16. Nishikawa T, Akiyoshi K, Sunamoto J (1996) Macromolecular complexation between bovine serum albumin and self-assembled hydrogel nanoparticle of hydrophobized polysaccharides. J Am Chem Soc 118:6110–6115

    Article  CAS  Google Scholar 

  17. Akiyoshi K, Sasaki Y, Sunamoto J (1999) Molecular chaperone-like activity of hydrogel nanoparticles of hydrophobized pullulan: thermal stabilization with refolding of carbonic anhydrase B. Bioconj Chem 10:321–324

    Article  CAS  Google Scholar 

  18. Nomura Y, Ikeda M, Yamaguchi N et al (2003) Protein refolding assisted by self-assembled nanogels as novel artificial molecular chaperone. FEBS Lett 553:271–276

    Article  CAS  Google Scholar 

  19. Nomura Y, Sasaki Y, Takagi M et al (2005) Thermoresponsive controlled association of protein with a dynamic nanogel of hydrophobized polysaccharide and cyclodextrin: heat shock protein-like activity of artificial molecular chaperone. Biomacromolecules 6:447–452

    Article  CAS  Google Scholar 

  20. Kopecek J (2002) Swell gels. Nature 417:388–390

    Article  CAS  Google Scholar 

  21. Hoffman AS (2002) Hydrogels for biomedical applications. Adv Drug Deliv Rev 54:3–12

    Article  CAS  Google Scholar 

  22. Byrne ME, Park K, Peppas NA (2002) Molecular imprinting within hydrogels. Adv Drug Deliv Rev 54:149–161

    Article  CAS  Google Scholar 

  23. Akiyoshi K, Kobayashi S, Shichibe S et al (1998) Self-assembled hydrogel nanoparticle of cholesterol-bearing pullulan as a carrier of protein drugs: complexation and stabilization of insulin. J Control Release 54:313–320

    Article  CAS  Google Scholar 

  24. Gu X-G, Schmitt M, Hiasa A et al (1998) A novel hydrophobized polysaccharide/oncoprotein complex vaccine induces in vitro and in vivo cellular and humoral immune responses against HER2 expressing murine sarcoma. Cancer Res 58:3385–3390

    CAS  Google Scholar 

  25. Ikuta Y, Katayama N, Wang L et al (2002) Presentation of a major histocompatibility complex class 1-binding peptide by monocyte-derived dendritic cells incorporating hydrophobized polysaccharide-truncated HER2 protein complex: implications for a polyvalent immuno-cell therapy. Blood 99:3717–3724

    Article  CAS  Google Scholar 

  26. Kitano S, Kageyama S, Nagata Y et al (2006) Induction of HER2-specific T Cell immune responses in patients vaccinated with truncated HER2 A. Clin Cancer Res 2:7397–7405

    Article  Google Scholar 

  27. Kageyama S, Kitano S, Hirayama M et al (2008) Humoral immune responses in patients vaccinated with HER2 protein complexed with cholesteryl pullulan nanogel (CHP-HER2). Cancer Sci 99:601–607

    Article  CAS  Google Scholar 

  28. Uenaka A, Wada H, Isobe M et al (2007) T cell immunomonitoring and tumor responses in patients immunized with a complex of cholesterol-bearing hydrophobized pullulan (CHP) and NY-ESO-1 protein. Cancer Immun 7:9–20

    Google Scholar 

  29. Alles N, Soysa NS, Mian AH et al (2009) Polysaccharide nanogel delivery of a TNF-α and RANKL antagonist peptide allows systemic prevention of bone loss. Eur J Pharm Sci 37:83–88

    Article  CAS  Google Scholar 

  30. Shimizu T, Kishida T, Hasegawa U et al (2008) Nanogel DDS enables sustained release of a cytokine for tumor immunotherapy. Biochem Biophys Res Commun 367:330–335

    Article  CAS  Google Scholar 

  31. Ayame H, Morimoto N, Akiyoshi K (2008) Self-assembled cationic nanogels for intracellular protein delivery system. Bioconj Chem 19:882–890

    Article  CAS  Google Scholar 

  32. Morimoto N, Tamada J, Sawada S et al (2009) Interaction of self-assembled cationic nanogels with oligo-dna and function as artificial nucleic acid chaperone. Chem Lett 38:496–497

    Article  CAS  Google Scholar 

  33. Sugawara A, Yamane S, Akiyoshi K (2006) Nanogel-templated mineralization: polymer-calcium phosphate hybrid nanomaterials. Macromol Rapid Commun 27:441–446

    Article  CAS  Google Scholar 

  34. Yamane S, Sugawara A, Sasaki Y et al (2009) Nanogel-calcium phosphate hybrid nanoparticles with negative or positive charges for potential biomedical applications. Bull Chem Soc Jpn 82:416–418

    Article  CAS  Google Scholar 

  35. Yamane S, Sugawara A, Watanabe A et al (2009) Hybrid nanoapatitby polysaccharide nanogel-templated mineralization. J Bioact Compat Polym 24:129–150

    Article  Google Scholar 

  36. Hasegawa U, Nomura SM, Kaul CS et al (2005) Nanogel-quantum dot hybrid nanoparticles for live cell imaging. Biochem Biophys Res Commun 331:917–921

    Article  CAS  Google Scholar 

  37. Toita S, Hasegawa U, Koga H et al (2008) Protein-conjugated QD effectively delivered into living cells by a cationic nanogel. J Nanosci Nanotechol 8:1–7

    Article  Google Scholar 

  38. Fukui T, Kobayashi H, Hasegawa U et al (2007) Intracellular delivery of nanogel-quantum dot hybrid nanoparticles into human periodontal ligament cells. Drug Metab Lett 1:131–135

    Article  CAS  Google Scholar 

  39. Hennink WE, van Nostrum CF (2002) Novel crosslinking methods to design hydrogels. Adv Drug Deliv Rev 54:13–36

    Article  CAS  Google Scholar 

  40. Morimoto N, Endo T, Iwasaki Y et al (2005) Design of hybrid hydrogels with self-assembled nanogels as cross-linkers: interaction with proteins and chaperone-like activity. Biomacromolecules 6:1829–1834

    Article  CAS  Google Scholar 

  41. Morimoto N, Ohki T, Kurita K et al (2008) Thermo-responsive hydrogels with nanodomains: rapid shrinking of nanogel-crosslinking hydrogel of poly (N-isopropyl acrylamide). Macromol Rapid Commun 29:672–676

    Article  CAS  Google Scholar 

  42. Kato N, Hasegawa U, Morimoto N et al (2007) Nanogel-based delivery system enhances PGE2 effects on bone formation. J Cell Biochem 101:1063–1070

    Article  CAS  Google Scholar 

  43. Hayashi C, Hasegawa U, Saita Y et al (2009) Osteoblastic bone formation is induced by using nanogel-crosslinking hydrogel as novel scaffold for bone growth factor. J Cell Phys 220:1–7

    Article  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Materials Processing, Graduate School of Engineering, Tohoku University, 6-6-02 Aramaki-aza Aoba, Aoba-ku, Sendai, 980-8579, Japan

    Nobuyuki Morimoto

  2. Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-surugadai, Chiyoda-ku, Tokyo, 101-0062, Japan

    Kazunari Akiyoshi

Authors
  1. Nobuyuki Morimoto
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  2. Kazunari Akiyoshi
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Corresponding author

Correspondence to Kazunari Akiyoshi .

Editor information

Editors and Affiliations

  1. Virginia Commonwealth University, Richmond, 23284, Virgin Islands, USA

    Raphael M. Ottenbrite

  2. Dept. Biomedical Engineering, Purdue University, West Lafayette, 47907-1296, Indiana, USA

    Kinam Park

  3. Tokyo Women's Medical College, Kawada-cho Ichigaya 8-1, Tokyo, 162-8666, Japan

    Teruo Okano

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Morimoto, N., Akiyoshi, K. (2010). Self-assembled Nanogel Engineering. In: Ottenbrite, R., Park, K., Okano, T. (eds) Biomedical Applications of Hydrogels Handbook. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-5919-5_18

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