Abstract
Plants contain secondary metabolites with antifungal properties. In fruits they are mostly concentrated in the peel at immature stage and decline during ripening in coincidence with fungal rot development. The information on antifungal systems in immature avocado and mango, reviewed here, suggests that they play a role in natural disease resistance. Immature mangoes have evolved a formidable antifungal system comprising several resorcinols, gallotannins and chitinases. Resorcinols and gallotannins are inhibitory to major postharvest pathogens, Colletotrichum gloeosporioides causing anthracnose and Botryodiplodia theobromae causing stem-end rot. Their levels are generally higher in resistant cultivars than in susceptible ones. Mango latex, distributed in a fine network of canals in the fruit peel, contains chitinases which have the ability to rapidly digest conidia of C. gloeosporioides. Gallotannins and resorcinols decline progressively during ripening and the latex disappears when ripe rot development begins. Retention of latex in the harvested fruit reduces anthracnose and stem-end rot development during ripening. Treatment of harvested fruit with CO2 or inoculation with certain non-pathogenic fungi increased antifungal resorcinol concentration. Immature avocado fruits possess a pre-formed antifungal system comprising at least five antifungal compounds. The quiescence of C. gloeosporioides in the immature fruit has been attributed to the pre-formed antifungal activity of the peel. Lipoxygenase activity increases during fruit ripening, while epicatechin levels decline, suggesting that these events are linked to the decrease in di-ene concentrations. Inhibition of lipoxygenase activity results in retention of antifungal di-ene during ripening increasing fruit resistance. In freshly harvested avocados, the di-ene concentration can be further enhanced by treatment with biotic and abiotic agents.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Adikaram NKB, Brown AE, Swinburne TR (1982) Phytoalexin involvement in the latent infection of Capsicum annuum L. fruit by Glomerella cingulata (Stonem.). Physiol Plant Pathol 21:161-170
Adikaram NKB, Ewing DF, Karunaratne AM, Wijeratne EMK (1992) Antifungal compounds from immature avocado fruit peel. Phytochemistry 31(1):93-96
Ardi R, Kobiler I, Jacoby B, Keen NT, Prusky D (1998) Involvement of epicatechin biosynthesis in the activation of the mechanism of resistance of avocado fruits to Colletotrichum gloeosporioides. Physiol Mol Plant Pathol 53:269-285
Bandyopadhyay C, Gholap AS, Mamdapur VR (1985) Characterization of alkenylresorcinols in mango (Mangifera indica L.) latex. J Agr Food Chem 33:377-379
Berardini N, Carle R, Schieber A (2004) Characterization of gallotannins and benzophenone derivatives from mango (Mangifera indica L. cv. Tommy Atkins) peel, pulp and kernels by high performance liquid chromatography/electrospray ionization mass spectrometry. Rapid Comm Mass Spectrom 18:2208-2216
Binyamini N, Schiffmann-Nadel M (1972) Latent infection in avocado fruit due to Colletotrichum gloeosporioides. Phytopathology 67:315-320
Coates LM, Muirhead IF, Irwin JAG, Gowanlock DH (1993) Initial infection process by Colletotrichum gloeosporioides. Mycol Res 97(1):1363-1370
Cojocaru M, Droby S, Glotter E, Goldman A, Gottlieb HE, Jacoby B, Prusky D (1986) 5-(12-heptadecenyl)-resorcinol, the major component of the antifungal activity in the peelof mango fruit. Phytochemistry 25(5):1093-1095
Droby S, Prusky D, Jacoby B, Goldman A (1986) Presence of antifungal compounds in the peel of mango fruits and their relation to latent infections of Alternaria alternate. Physiol Mol Plant Pathol 29:173-183
Droby S, Prusky D, Jacoby B, Goldman A (1987) Induction of antifungal resorcinols in unripe mango fruits and its relation to latent infection by Alternaria alternata. Physiol Mol Plant Pathol 30:285-292
Hall R (1971) Pathogenicity of Monilinia fructicola. Part II. Penetration of peach leaf and fruit. Phytopathologische zeitschrift 72:281-290
Hassan KM (2006) Constitutive alk(en)ylresorcinols and resistance to postharvest diseases in mango (Mangifera indica L.). Ph.D. thesis, University of Queensland, Australia, pp 229
Hassan MK, Dann EK, Irving DE, Coates LM (2007) Concentrations of constitutive alk(en)ylresorcinols in peel of commercial mango varieties and resistance to postharvest anthracnose. Physiol Mol Plant Pathol 71:158-165
Kabuki T, Nakajima H, Arai M, Ueda S, Kawabara Y, Dosako S (2000) Characterization of navel antimicrobial compounds from mango (Mangifera indica) kernal seeds. Food Chem 71:61
Karunanayake KOLC (2008) Natural defence mechanisms in mango fruit and their potential in the management of postharvest diseases. Ph.D. Thesis, University of Peradeniya, Sri Lanka, pp 297
Knodler M, Berardini N, Kammerer RD, Carle R, Sciber A (2007) Characterization of major and minor alk(en)yl resorcinols from mango (Mangifera indica L.) peels by high-performance liquid chromatography/atomic pressure chemical ionization mass spectrometry. Rapid Comm Mass Spectrom 21:945-951
Kobiler I, Reved R, Artez L, Prusky D (1998) Antifungal compounds regulating postharvest diseases in mango. In: Johnson GI, Highley E, Joyce DC (eds) Disease resistance in fruit. ACIAR Proceedings No. 80, 109-114
Mayer AM (1987) Polyphenol oxidases in plants - recent progress. Phytochemistry 26(1):11-20
Muirhead IF, Deverall BJ (1981) Role of appressoria in latent infection of banana fruits by Colletotrichum musae. Physiol Plant Pathol 19:77-84
Oka K, Saito F, Yasuhara T, Sugimoto A (2004) A study of cross-reactions between mango contact allergens and urushiol. Contact Dermatitis 51:292-296
Osbourn AE (1996) Saponins and plant defence - a soap story. Trends Plant Sci 1:4-9
Prusky D, Keen NT (1993) Involvement of preformed antifungal compounds in the resistance of subtropical fruits to fungal decay. Plant Dis 77:114-119
Prusky D, Keen NT (1995) Inducible preformed compounds and their involvement in the resistance of plants to pathogens. In: Reuveni R (ed) Novel approaches to integrated pest management. Lewis Publishers, Boca Raton, FL, pp 139-151
Prusky D, Keen NT, Sims JJ, Midland SL (1982) Possible involvement of an antifungal diene in the latency of Colletotrichum gloeosporioides on unripe avocado fruit. Phytopathology 72:1578-1582
Prusky D, Keen NT, Eaks I (1983) Further evidence for the involvement of a pre-formed antifungal compound in the latency of Colletotrichum gloeosporioides on unripe avocado fruits. Physiol Mol Plant Pathol 22:189-198
Prusky D, Kobiler I, Jacoby B, Sims JJ, Midland SL (1985) Inhibitors of avocado lipoxygenase; their possible relationship with the latency of Colletotrichum gloeosporioides. Physiol Mol Plant Pathol 27:269-279
Prusky D, Karni L, Kobiler L, Plumbley RA (1990) Induction of the antifungal diene in unripe avocado fruits: effect of inoculation with Colletotrichum gloeosporioides. Physiol Mol Plant Pathol 37:425-435
Prusky D, Kobiler I, Fishman Y, Sims JJ, Midland SL, Keen NT (1991) Identification of an antifungal compound in unripe avocado fruits and its possible involvement in the quiescent infections of Colletotrichum gloeosporioides. J Phytopathol 132:319-327
Prusky D, Freeman S, Rodriguez R, Keen NT (1994) A nonpathogenic mutant strain of Colletotrichum magna induces resistance to Colletotrichum gloeosporioides in avocado fruits. Mol Plant Microbe Interact 7:326-333
Prusky D, Wattad C, Kobiler I (1996) Effect of ethylene on the activation of quiescent infection of Colletotrichum gloeosporioides in avocado fruits. Mol Plant Microbe Interact 9:864-868
Schonbeck F, Schlosser E (1976) Preformed substances as potential protectants. In: Heitefuss R, Williams PH (eds) Physiological Plant Pathology. Springer-Verlag, Berlin, pp 653-678
Sivanathan S, Adikaram NKB (1989) Biological activity of four antifungal compounds in immature avocado. J Phytopathol 125:97-109
VanEtten HD, Mansfield JW, Bailey JA, Farmer EE (1994) Letter to the editor. Two classes of plant antibiotics: phytoalexins versus phytoanticipins Plant Cell 6:1191-1192
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Springer Science+Business Media B.V.
About this chapter
Cite this chapter
Adikaram, N., Karunanayake, C., Abayasekara, C. (2009). The Role of Pre-formed Antifungal Substances in the Resistance of Fruits to Postharvest Pathogens. In: Prusky, D., Gullino, M. (eds) Postharvest Pathology. Plant Pathology in the 21st Century, vol 2. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8930-5_1
Download citation
DOI: https://doi.org/10.1007/978-1-4020-8930-5_1
Published:
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-8929-9
Online ISBN: 978-1-4020-8930-5
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)