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In situ Hybridization Histochemistry as a Tool for the Study of Brain Function

  • Chapter
Current Aspects of the Neurosciences

Abstract

In situ hybridization histochemistry has become a powerful tool for the study of specific peptide or protein biosynthesis at the level of the single neuron. Although this technique has also frequently been used to confirm immunoreactive neurons as the actual site of biosynthesis (e.g. Gee et al., 1983), the greatest utility is apparent in studies of altered gene expression in development or pathology, or due to physiological or pharmacological manipulations (Gee and Roberts, 1983). The goal of this chapter is to provide the reader with essential background information for understanding the technology of in situ hybridization histochemistry and then describe some of its applications, to help the reader to appreciate the potential utility of this technology for the analysis of brain function and its disorders.

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  1. Cephalon, Inc., 145 Brandywine Parkway, West Chester, PA 19380, USA

    Frank Baldino Jr, Jill M. Roberts-Lewis & Michael E. Lewis

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  1. Frank Baldino Jr
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  2. Jill M. Roberts-Lewis
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  1. Nuffield Laboratory of Ophthalmology, University of Oxford, OX2 6AW, Oxford, UK

    Neville N. Osborne

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© 1992 Macmillan Publishers Limited

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Baldino, F., Roberts-Lewis, J.M., Lewis, M.E. (1992). In situ Hybridization Histochemistry as a Tool for the Study of Brain Function. In: Osborne, N.N. (eds) Current Aspects of the Neurosciences. Palgrave, London. https://doi.org/10.1007/978-1-349-12275-2_1

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