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Handbook of Neurochemistry and Molecular Neurobiology pp 737–769Cite as

7.1 Proteomics

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Abstract:

Proteomics is the study of the proteome defined as the set of all proteins of a cell, an organ, or the whole organism. Currently, the proteomic approach usually combines two-dimensional (2D) gel electrophoresis and mass spectrometry (MS) as a common and powerful approach. The specific feature of proteomics is the simultaneous detection of many proteins involved in different biochemical pathways. With regard to the brain, this approach has proven to be particularly beneficial for the analysis of energy metabolism and cellular respiration. To gather proteomic information, techniques were developed to handle the large amount of data yielding a comprehensive pattern of protein alignment within brain biochemical pathways. It is expected that in future proteomics will become a routine tool in all aspects of brain cell biology and beyond those of bioenergetics, including cell development, differentiation, and cell death. Proteomics then will complement other approaches to analyze cerebral function and biochemistry under normal and disease conditions.

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Acknowledgments

This work was supported by the German Ministry of Education and Research (BMBF) in the research programs Competence Network Stroke (B2, to WK) and the National Genome Research Network NGFN-2 (N3NV-S19T05, to MHM and WK), as well as by the German Research Foundation DFG (MA 2492/2-2, to MHM and WK).

Authors
  1. M. H. Maurer
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  2. W. Kuschinsky
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Editors and Affiliations

  1. Center for Neurochemistry, Nathan S. Kline Institute for Psychiatric Research, 140 Old Orangeburg Road, 10962, Orangeburg, Newyork, USA

    Abel Lajtha (Director) (Director)

  2. Weill Medical College of Cornell University, Burke Medical Research Institute, 785 Mamaroneck Avenue, White Plains, NY, 10605, USA

    Gary E. Gibson Ph.D. (Professor of Neuroscience Department of Neurology and Neuroscience) (Professor of Neuroscience Department of Neurology and Neuroscience)

  3. Dept. Neurology and Physiology & Biophysics, University Arkansas for Medical Sciences, Shorey Bldg. Room 715, Mail Slot 830 4301 W. Markham St., Little Rock, AR, 72205, USA

    Gerald A. Dienel Ph.D. (Professor) (Professor)

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Maurer, M.H., Kuschinsky, W. (2007). 7.1 Proteomics. In: Lajtha, A., Gibson, G.E., Dienel, G.A. (eds) Handbook of Neurochemistry and Molecular Neurobiology. Springer, Boston, MA. https://doi.org/10.1007/978-0-387-30411-3_27

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