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Cytoskeletal and Vacuolar Dynamics During Plant Cell Division: Approaches Using Structure-Visualized Cells

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Cell Division Control in Plants

Part of the book series: Plant Cell Monographs ((CELLMONO,volume 9))

Abstract

During cell cycle progression, intra-cellular cytoskeletal and membrane structures undergo dynamicchanges in their form and localization, which in turn regulate further progress of the cell cycle. Despitethe considerable insights into these intra-cellular structures obtained from immuno-fluorescence microscopy,the need for chemical fixation has limited the acquired images to only static ones. In contrast, more recentfluorescent protein techniques used to visualize these structures in living cell systems have allowed investigationsof their dynamics. The visualization of microtubules (MTs) by using the green fluorescent protein (GFP)and the analysis of MT-associated proteins will be presented. In addition, to further understand plantcell cycle progression, dynamics of actin microfilaments (MFs) and vacuolar membranes (VMs) visualized withfluorescent proteins are also reviewed.

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Authors and Affiliations

  1. Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha, 277-8562, Kashiwa, Japan

    Toshio Sano, Natsumaro Kutsuna, Takumi Higaki, Yoshihisa Oda, Arata Yoneda & Seiichiro Hasezawa

  2. Faculty of Education, Gunma University, Aramaki, 371-8510, Maebashi, Japan

    Fumi Kumagai-Sano

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  1. Toshio Sano
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  2. Natsumaro Kutsuna
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  3. Takumi Higaki
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Correspondence to Toshio Sano .

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Sano, T. et al. (2007). Cytoskeletal and Vacuolar Dynamics During Plant Cell Division: Approaches Using Structure-Visualized Cells. In: Verma, D.P.S., Hong, Z. (eds) Cell Division Control in Plants. Plant Cell Monographs, vol 9. Springer, Berlin, Heidelberg. https://doi.org/10.1007/7089_2007_125

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