Skip to main content
SpringerLink
Log in
Book cover

Reviews of Environmental Contamination and Toxicology Volume 241 pp 1–38Cite as

Gene Expression Profiling in Fish Toxicology: A Review

  • Chapter

Part of the book series: Reviews of Environmental Contamination and Toxicology ((RECT,volume 241))

Abstract

In this review, we present an overview of transcriptomic responses to chemical exposures in a variety of fish species. We have discussed the use of several molecular approaches such as northern blotting, differential display reverse transcription-polymerase chain reaction (DDRT-PCR), suppression subtractive hybridization (SSH), real time quantitative PCR (RT-qPCR), microarrays, and next-generation sequencing (NGS) for measuring gene expression. These techniques have been mainly used to measure the toxic effects of single compounds or simple mixtures in laboratory conditions. In addition, only few studies have been conducted to examine the biological significance of differentially expressed gene sets following chemical exposure. Therefore, future studies should focus more under field conditions using a multidisciplinary approach (genomics, proteomics and metabolomics) to understand the synergetic effects of multiple environmental stressors and to determine the functional significance of differentially expressed genes. Nevertheless, recent developments in NGS technologies and decreasing costs of sequencing holds the promise to uncover the complexity of anthropogenic impacts and biological effects in wild fish populations.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   119.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

References

  • Adedeji OB, Durhan EJ, Garcia-Reyero N, Kahl MD, Jensen KM, Lalone CA, Makynen EA, Perkins EJ, Thomas L, Villeneuve DL, Ankley GT (2012) Short-term study investigating the estrogenic potency of diethylstilbesterol in the fathead minnow (Pimephales promelas). Environ Sci Technol 46:7826–7835

    CAS  Google Scholar 

  • Alwine JC, Kemp DJ, Stark GR (1977) Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc Natl Acad Sci U S A 74:5350–5354

    CAS  Google Scholar 

  • American Public Health Association, American Water Works Association, Water Pollution Control Federation (1960) Publication of “Bioassay methods for the evaluation of acute toxicity of industrial wastes and other substances to fish” in the 11th edition of Standard Methods. New York, p 626

    Google Scholar 

  • Ankley GT, Villeneuve DL (2006) The fathead minnow in aquatic toxicology: past, present and future. Aquat Toxicol 78:91–102

    CAS  Google Scholar 

  • Arini A, Gourves PY, Gonzalez P, Baudrimont M (2015) Metal detoxification and gene expression regulation after a Cd and Zn contamination: an experimental study on Danio rerio. Chemosphere 128:125–133

    CAS  Google Scholar 

  • Arukwe A, Knudsen FR, Goksøyr A (1997) Fish zona radiata (eggshell) protein: a sensitive biomarker for environmental estrogens. Environ Health Perspect 105:418–422

    CAS  Google Scholar 

  • Arukwe A, Kullman SW, Hinton DE (2001) Differential biomarker gene and protein expressions in nonylphenol and estradiol-17 beta treated juvenile rainbow trout (Oncorhynchus mykiss). Comp Biochem Physiol C Toxicol Pharmacol 129:1–10

    CAS  Google Scholar 

  • Arukwe A, Kullman SW, Berg K, Goksoyr A, Hinton DE (2002) Molecular cloning of rainbow trout (Oncorhynchus mykiss) eggshell zona radiata protein complementary DNA: mRNA expression in 17beta-estradiol- and nonylphenol-treated fish. Comp Biochem Physiol B Biochem Mol Biol 132:315–326

    Google Scholar 

  • Baer CE, Ippolito DL, Hussainzada N, Lewis JA, Jackson DA, Stallings JD (2014) Genome-wide gene expression profiling of acute metal exposures in male zebrafish. Genom Data 2:363–365

    Google Scholar 

  • Bahamonde PA, McMaster ME, Servos MR, Martyniuk CJ, Munkittrick KR (2015) Molecular pathways associated with the intersex condition in rainbow darter (Etheostoma caeruleum) following exposures to municipal wastewater in the Grand River basin, ON, Canada. Part B. Aquat Toxicol 159:302–316

    CAS  Google Scholar 

  • Boon JP, van Zanden JJ, Lewis WE, Zegers BN, Goksøyr A, Arukwe A (2002) The expression of CYP1A, vitellogenin and zona radiata proteins in Atlantic salmon (Salmo salar) after oral dosing with two commercial PBDE flame retardant mixtures: absence of short-term responses. Mar Environ Res 54:719–724

    CAS  Google Scholar 

  • Bowman CJ, Kroll KJ, Hemmer MJ, Folmar LC, Denslow ND (2000) Estrogen-induced vitellogenin mRNA and protein in sheepshead minnow (Cyprinodon variegatus). Gen Comp Endocrinol 120:300–313

    CAS  Google Scholar 

  • Bowman CJ, Kroll KJ, Gross TG, Denslow ND (2002) Estradiol-induced gene expression in largemouth bass (Micropterus salmoides). Mol Cell Endocrinol 196:67–77

    CAS  Google Scholar 

  • Brazma A, Parkinson H, Sarkans U, Shojatalab M, Vilo J, Abeygunawardena N, Holloway E, Kapushesky M, Kemmeren P, Lara GG, Oezcimen A, Rocca-Serra P, Sansone S-A (2003) ArrayExpress-a public repository for microarray gene expression data at the EBI. Nucleic Acids Res 31:68–71

    CAS  Google Scholar 

  • Brown M, Robinson C, Davies IM, Moffat CF, Redshaw J, Craft JA (2004) Temporal changes in gene expression in the liver of male plaice (Pleuronectes platessa) in response to exposure to ethynyl oestradiol analysed by macroarray and Real-Time PCR. Mutat Res 552:35–49

    CAS  Google Scholar 

  • Buhler DR, Miranda CL, Henderson MC, Yang Y-H, Lee S-J, Wang-Buhler J-L (2000) Effects of 17β-estradiol and testosterone on hepatic mRNA/protein levels and catalytic activities of CYP2M1, CYP2K1, and CYP3A27 in rainbow trout (Oncorhynchus mykiss). Toxicol Appl Pharmacol 168:91–101

    CAS  Google Scholar 

  • Cai Y, Li H, Hao L, Li G, Xie P, Chen J (2012) Identification of cda gene in bighead carp and its expression in response to microcystin-LR. Ecotoxicol Environ Saf 79:206–213

    CAS  Google Scholar 

  • Camargo MM, Martinez CB (2007) Histopathology of gills, kidney and liver of a Neotropical fish caged in an urban stream. Neotrop Ichthyol 5:327–336

    Google Scholar 

  • Carginale V, Capasso C, Scudiero R, Parisi E (2002) Identification of cadmium-sensitive genes in the Antarctic fish Chionodraco hamatus by messenger RNA differential display. Gene 299:117–124

    CAS  Google Scholar 

  • Causton HC, Ren B, Koh SS, Harbison CT, Kanin E, Jennings EG, Lee TI, True HL, Lander ES, Young RA (2001) Remodeling of yeast genome expression in response to environmental changes. Mol Biol Cell 12:323–337

    CAS  Google Scholar 

  • Celius T, Matthews JB, Giesy JP, Zacharewski TR (2000) Quantification of rainbow trout (Oncorhynchus mykiss) zona radiata and vitellogenin mRNA levels using real-time PCR after in vivo treatment with estradiol-17β or α-zearalenol. J Steroid Biochem Mol Biol 75:109–119

    CAS  Google Scholar 

  • Chan KM, Ku LL, Chan PC-Y, Cheuk WK (2006) Metallothionein gene expression in zebrafish embryo-larvae and ZFL cell-line exposed to heavy metal ions. Mar Environ Res 62:S83–S87

    CAS  Google Scholar 

  • Chen TT (1983) Identification and characterization of estrogen-responsive gene products in the liver of rainbow trout. Can J Biochem Cell Biol 61:802–810

    CAS  Google Scholar 

  • Chen J (2015) Cardiac toxicity by sublethal 2,3,7,8-tetrachlorodibenzo-p-dioxin correlates with its anti-proliferation effect on cardiomyocytes in zebrafish embryos. Environ Toxicol Chem 34(2):420–428

    CAS  Google Scholar 

  • Cheung APL, Lam TH-J, Chan KM (2004) Regulation of Tilapia metallothionein gene expression by heavy metal ions. Mar Environ Res 58:389–394

    CAS  Google Scholar 

  • Chow WS, Chan WK-L, Chan KM (2011) Toxicity assessment and vitellogenin expression in zebrafish (Danio rerio) embryos and larvae acutely exposed to bisphenol A, endosulfan, heptachlor, methoxychlor and tetrabromobisphenol A. J Appl Toxicol 33:670–678

    Google Scholar 

  • Colli-Dula R, Zuniga-Aguilar J-J, Albores-Medina A, Zapata-Perez O (2009) Identification of genes expressed as a result of lindane exposure in Oreochromis niloticus using differential display. Ecotoxicol Environ Saf 72:1406–1412

    CAS  Google Scholar 

  • Conti GO, Copat C, Ledda C, Fiore M, Fallico R, Sciacca S, Ferrante M (2012) Evaluation of heavy metals and polycyclicaromatichydrocarbons (PAHs) in Mullus barbatus from Sicily Channel and risk-based consumption limits. Bull Environ Contam Toxicol 88:946–950

    CAS  Google Scholar 

  • Copat C, Bella F, Castaing M, Fallico R, Sciacca S, Ferrante M (2012a) Heavy metals concentrations in fish from Sicily (Mediterranean Sea) and evaluation of possible health risks to consumers. Bull Environ Contam Toxicol 88:78–83

    CAS  Google Scholar 

  • Copat C, Maggiore R, Arena G, Lanzafame S, Fallico R, Sciacca S, Ferrante M (2012b) Evaluation of a temporal trend heavy metals contamination in Posidonia oceanica (L.) Delile, (1813) along the western coastline of Sicily (Italy). J Environ Monit 14:187–192

    CAS  Google Scholar 

  • Cruz FF, Leite CE, Pereira TCB et al (2013) Assessment of mercury chloride-induced toxicity and the relevance of P2X7 receptor activation in zebrafish larvae. Comp Biochem Physiol C Toxicol Pharmacol 158:159–164

    CAS  Google Scholar 

  • Dangre AJ, Manning S, Brouwer M (2010) Effects of cadmium on hypoxia-induced expression of hemoglobin and erythropoietin in larval sheepshead minnow, Cyprinodon variegatus. Aquat Toxicol 99:168–175

    CAS  Google Scholar 

  • Davis AP, Grondin CJ, Lennon-Hopkins K, Saraceni-Richards C, Sciaky D, King BL, Wiegers TC, Mattingly CJ (2015) The Comparative Toxicogenomics Database’s 10th year anniversary: update 2015. Nucleic Acids Res 43:D914–D920

    CAS  Google Scholar 

  • Denslow ND, Chow M, Chow MM, Bonomelli S, Folmar LC, Heppell SA, Sullivan SV (1997a) Development of biomarkers for environmental contaminants affecting fish. In: Rolland RM, Gilbertson M, Peterson RE (eds) Chemically induced alterations in functional development and reproduction of fishes, Proceeding from a session at the 1995 Wingspread Conference, Society of Environmental Toxicology and Chemistry (SETAQ). Publication Series. SETAC Press, Pensacola, pp 73–86

    Google Scholar 

  • Denslow ND, Chow MM, Folmar LC, Bonomelli SL, Heppell SA, Sullivan CV (1997b) Development of antibodies to teleost vitellogenins: potential biomarkers for environmental estrogens. In: Bengston DA, Hen-schel DS (eds) Environmental toxicology and risk assessment: biomarkers and risk assessment, vol 5. American Society for Testing and Materials, Philadelphia, pp 23–36, ASTM STP 1306

    Google Scholar 

  • Denslow ND, Bowman CJ, Ferguson RJ, Lee HS, Hemmer MJ, Folmar LC (2001a) Induction of gene expression in sheepshead minnows (Cyprinodon variegatus) treated with 17 beta-estradiol, diethylstilbesterol, or ethinylestradiol: the use of mRNA fingerprints as an indicator of gene regulation. Gen Comp Endocrinol 121:250–260

    CAS  Google Scholar 

  • Denslow ND, Lee HS, Bowman CJ, Hemmer MJ, Folmar LC (2001b) Multiple responses in gene expression in fish treated with estrogen. Comp Biochem Physiol B Biochem Mol Biol 129:277–282

    CAS  Google Scholar 

  • Denslow ND, Kocerha J, Sepulveda MS, Gross T, Holm SE (2004) Gene expression fingerprints of largemouth bass (Micropterus salmoides) exposed to pulp and paper mill effluents. Mutat Res 552:19–34

    CAS  Google Scholar 

  • Diatchenko L, Lau YF, Campbell AP et al (1996) Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proc Natl Acad Sci U S A 93:6025–6030

    CAS  Google Scholar 

  • Dixon TJ, Taggart JB, George SG (2002) Application of real time PCR determination to assess inter-animal variabilities in CYP1A induction in the European flounder (Platichthys flesus). Mar Environ Res 54:267–270

    CAS  Google Scholar 

  • Du F, Xu G, Nie Z, Xu P, Gu R (2014) Transcriptome analysis gene expression in the liver of Coilia nasus during the stress response. BMC Genomics 15:558

    Google Scholar 

  • Du Z, Wang G, Gao S, Wang Z (2015) Aryl organophosphate flame retardants induced cardiotoxicity during zebrafish embryogenesis: by disturbing expression of the transcriptional regulators. Aquat Toxicol 161:25–32

    CAS  Google Scholar 

  • Edgar R, Domrachev M, Lash AE (2002) Gene Expression Omnibus: NCBI gene expression and hybridization array data repository. Nucleic Acids Res 30:207–210

    CAS  Google Scholar 

  • Fan C-Y, Cowden J, Simmons SO, Padilla S, Ramabhadran R (2010) Gene expression changes in developing zebrafish as potential markers for rapid developmental neurotoxicity screening. Neurotoxicol Teratol 32:91–98

    CAS  Google Scholar 

  • Ferraris M, Flora A, Chiesara E, Fornasari D, Lucchetti H, Marabini L, Frigerio S, Radice S (2005) Molecular mechanism of the aryl hydrocarbon receptor activation by the fungicide iprodione in rainbow trout (Oncorhynchus mykiss) hepatocytes. Aquat Toxicol 72:209–220

    CAS  Google Scholar 

  • Flouriot G, Pakdel F, Ducouret B, Ledrean Y, Valotaire Y (1997) Differential regulation of two genes implicated in fish reproduction: vitellogenin and estrogen receptor genes. Mol Reprod Dev 48:317–323

    CAS  Google Scholar 

  • Folmar LC, Denslow ND, Rao V, Chow M, Crain DA, Enblom J, Marcino J, Guillette LJ Jr (1996) Vitellogenin induction and reduced serum testosterone concentrations in feral male carp, Cyprinus carpio. Captured near a major metropolitan sewage treatment plant. Environ Health Perspect 104:1096–1101

    CAS  Google Scholar 

  • Funkenstein B, Bowman C, Denslow ND, Cardinali M, Carnevali O (2000) Contrasting effects of estrogen on transthyretin and vitellogenin expression in males of the marine fish, Sparus aurata. Mol Cell Endocrinol 167:33–41

    CAS  Google Scholar 

  • Funkenstein B (2001) Developmental expression, tissue distribution and hormonal regulation of fish (Sparus aurata) serum retinol-binding protein. Comp Biochem Physiol B Biochem Mol Biol 129:613–622

    CAS  Google Scholar 

  • Gagné F, André C, Skirrow R, Gélinas M, Auclair J, van Aggelen G, Turcotte P, Gagnon C (2012) Toxicity of silver nanoparticles to rainbow trout: a toxicogenomic approach. Chemosphere 89:615–622

    Google Scholar 

  • Garcia de la serrana D, Estévez A, Andree K, Johnston IA (2012) Fast skeletal muscle transcriptome of the Gilthead sea bream (Sparus aurata) determined by next generation sequencing. BMC Genomics 13:181

    Google Scholar 

  • Garcia-Reyero N, Denslow ND (2006) Applications of genomic technologies to the study of organochlorine pesticide-induced reproductive toxicity in fish. J Pestic Sci 31(3):252–262

    CAS  Google Scholar 

  • Garcia-Reyero N, Griffitt RJ, Liu L, Kroll KJ, Farmerie WG, Barber DS, Denslow ND (2008) Construction of a robust microarray from a non-model species (largemouth bass) using pyrosequencing technology. J Fish Biol 72:2354–2376

    Google Scholar 

  • Geoghegan F, Katsiadaki I, Williams TD, Chipman JK (2008) A cDNA microarray for the three-spined stickleback, Gasterosteus aculeatus L, and analysis of the interactive effects of oestradiol and dibenzanthracene exposures. J Fish Biol 72:2133–2153

    CAS  Google Scholar 

  • Glisic B, Hrubik J, Fa S, Dopudj N, Kovacevic R, Andric N (2016) Transcriptional profiles of glutathione-S transferase isoforms, Cyp, and AOE genes in atrazine-exposed zebrafish embryos. Environ Toxicol 31:233–244

    CAS  Google Scholar 

  • Goodale BC, Tilton SC, Corvi MM, Wilson GR, Janszen DB, Anderson KA, Waters KM, Tanguay RL (2013) Structurally distinct polycyclic aromatic hydrocarbons induce differential transcriptional responses in developing zebrafish. Toxicol Appl Pharmacol 272:656–670

    CAS  Google Scholar 

  • Gracey AY, Troll JV, Somero GN (2001) Hypoxia-induced gene expression profiling in the euryoxic fish Gillichthys mirabilis. Proc Natl Acad Sci U S A 98:1993–1998

    CAS  Google Scholar 

  • Griffitt RJ, Hyndman K, Denslow ND, Barber DS (2009) Comparison of molecular and histological changes in zebrafish gills exposed to metallic nanoparticles. Toxicol Sci 107:404–415

    CAS  Google Scholar 

  • Gust KA, Brasfield SM, Stanley JK, Wilbanks MS, Chappell P, Perkins EJ, Lotufo GR, Lance RF (2011) Genomic investigation of year-long and multigenerational exposures of fathead minnow to the munitions compound RDX. Environ Toxicol Chem 30:1852–1864

    CAS  Google Scholar 

  • Gutiérrez-Praena D, Jos A, Pichardo S, Puerto M, Cameán AM (2013) Influence of the exposure way and the time of sacrifice on the effects induced by a single dose of pure Cylindrospermopsin on the activity and transcription of glutathione peroxidase and glutathione-S-transferase enzymes in Tilapia (Oreochromis niloticus). Chemosphere 90:986–992

    Google Scholar 

  • Hagenaars A, Knapen D, Meyer IJ, van der Ven K, Hoff P, De Coen W (2008) Toxicity evaluation of perfluorooctane sulfonate (PFOS) in the liver of common carp (Cyprinus carpio). Aquat Toxicol 88:155–163

    CAS  Google Scholar 

  • Hermsen SAB, Pronk TE, van den Brandhof E-J, van der Ven LTM, Piersma AH (2012) Concentration-response analysis of differential gene expression in the zebrafish embryo toxicity test following flusilazole exposure. Toxicol Sci 127(1):303–312

    CAS  Google Scholar 

  • Higuchi R, Fockler C, Dollinger G, Watson R (1993) Kinetic PCR analysis: real-time monitoring of DNA amplification reactions. Biotechnology 11:1026–1030

    CAS  Google Scholar 

  • Hofsteen P, Mehta V, Kim M-S, Peterson RE, Heideman W (2013) TCDD inhibits heart regeneration in adult zebrafish. Toxicol Sci 132(1):211–221

    CAS  Google Scholar 

  • Hogan NS, Wartman CA, Finley MA, van der Lee JG, van den Heuvel MR (2008) Simultaneous determination of androgenic and estrogenic endpoints in the threespine stickleback (Gasterosteus aculeatus) using quantitative RT-PCR. Aquat Toxicol 90:269–276

    CAS  Google Scholar 

  • Hong HN, Kim HN, Park KS, Lee S-K, Gu MB (2007) Analysis of the effects diclofenac has on Japanese medaka (Oryzias latipes) using real-time PCR. Chemosphere 67:2115–2121

    CAS  Google Scholar 

  • Hook SE, Skillman AD, Small JA, Schultz IR (2006) Gene expression patterns in rainbow trout, Oncorhynchus mykiss, exposed to a suite of model toxicants. Aquat Toxicol 77:372–385

    CAS  Google Scholar 

  • Huang Q, Dong S, Fang C, Wu X, Ye T, Lin Y (2012) Deep sequencing-based transcriptome profiling analysis of Oryzias melastigma exposed to PFOS. Aquat Toxicol 120–121:54–58

    Google Scholar 

  • Hunn JB (1989) History of acute toxicity tests with fish, 1863–1987. In: Investigations in fish control 98. US Fish and Wildlife Service, LaCrosse, WI, pp 1–10

    Google Scholar 

  • Ikeo K, Ishi-i J, Tamura T, Gojobori T, Tateno Y (2003) CIBEX: Center for Information Biology gene EXpression database. C R Biol 326:1079–1082

    CAS  Google Scholar 

  • Jayasundara N, Garner LV, Meyer JN, Erwin KN, Di Giulio RT (2015) AHR2-mediated transcriptomic responses underlying the synergistic cardiac developmental toxicity of PAHs. Toxicol Sci 143(2):469–481

    CAS  Google Scholar 

  • Jenny MJ, Aluru N, Hahn ME (2012) Effects of short-term exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin on microRNA expression in zebrafish embryos. Toxicol Appl Pharmacol 264:262–273

    CAS  Google Scholar 

  • Jiang W, Yang Y, Zhao D, Liu X, Duan J, Xie S, Zhao H (2011) Effects of sexual steroids on the expression of foxl2 in Gobiocypris rarus. Comp Biochem Physiol B Biochem Mol Biol 160:187–193

    CAS  Google Scholar 

  • Jing J, Liu H, Chen H, Hu S, Xiao K, Ma X (2013) Acute effect of copper and cadmium exposure on the expression of heat shock protein 70 in the Cyprinidae fish Tanichthys albonubes. Chemosphere 91:1113–1122

    CAS  Google Scholar 

  • Jobling S, Sheahan D, Osborne JA, Matthiessen P, Sumpter JP (1996) Inhibition of testicular growth in rainbow trout (Oncorhynchus mykiss) exposed to estrogenic alkylphenolic chemicals. Environ Toxicol Chem 15:194–202

    CAS  Google Scholar 

  • Jovanovic B, Ji T, Palic D (2011) Gene expression of zebrafish embryos exposed to titanium dioxide nanoparticles and hydroxylated fullerenes. Ecotoxicol Environ Saf 74:1518–1525

    CAS  Google Scholar 

  • Ju Z, Wells MC, Walter RB (2007) DNA microarray technology in toxicogenomics of aquatic models: methods and applications. Comp Biochem Physiol C Toxicol Pharmacol 145:5–14

    Google Scholar 

  • Jung J-H, Shim W, Addison RF, Baek J, Han C-H (2006) Protein and gene expression of VTG in response to 4-nonylphenol in rockfish (Sebastes schlegeli). Comp Biochem Physiol C Toxicol Pharmacol 143:162–170

    Google Scholar 

  • Klaper R, Rees CB, Drevnick P, Weber D, Sandheinrich M, Carvan MJ (2006) Gene expression changes related to endocrine function and decline in reproduction in fathead minnow (Pimephales promelas) after dietary methylmercury exposure. Environ Health Perspect 114(9):1337–1343

    CAS  Google Scholar 

  • Klaper R, Carter BJ, Richter CA, Drevnick PE, Sandheinrich MB, Tillitt DE (2008) Use of a 15 k gene microarray to determine gene expression changes in response to acute and chronic methylmercury exposure in the fathead minnow Pimephales promelas Rafinesque. J Fish Biol 72:2207–2280

    CAS  Google Scholar 

  • Knoebl I, Blum JL, Hemmer MJ, Denslow ND (2006) Temporal gene induction patterns in sheepshead minnows exposed to 17β-estradiol. J Exp Zool 305A:707–719

    CAS  Google Scholar 

  • Kriegsfeld LJ, Mei DF, Bentley GE, Ubuka T, Mason AO, Inoue K, Ukena K, Tsutsui K, Silver R (2006) Identification and characterization of a gonadotropin-inhibitory system in the brains of mammals. Proc Natl Acad Sci U S A 103:2410–2415

    CAS  Google Scholar 

  • Kure EH, Sæbø M, Stangeland AM, Hamfjord J, Hytterød S, Heggenes J, Lydersen E (2013) Molecular responses to toxicological stressors: profiling microRNAs in wild Atlantic salmon (Salmo salar) exposed to acidic aluminum-rich water. Aquat Toxicol 138–139:98–104

    Google Scholar 

  • Lam SH, Hlaing MM, Zhang X, Yan C, Duan Z, Zhu L, Ung CY, Mathavan S, Ong CN, Gong Z (2011) Toxicogenomic and phenotypic analyses of bisphenol-A early-life exposure toxicity in zebrafish. PLoS One 6(12):e28273

    CAS  Google Scholar 

  • Larkin P, Folmar LC, Hemmer MJ, Poston AJ, Denslow ND (2003) Expression profiling of estrogenic compounds using a sheepshead minnow cDNA macroarray. Environ Health Perspect 111:839–846

    CAS  Google Scholar 

  • Lazier CB, Langley S, Ramsey NB, Wright JM (1996) Androgen inhibition of vitellogenin gene expression in tilapia (Oreochromis niloticus). Gen Comp Endocrinol 104:321–329

    CAS  Google Scholar 

  • Leaver MJ, George SG (2000) A cytochrome P4501B gene from a fish, Pleuronectes platessa. Gene 256:83–91

    CAS  Google Scholar 

  • Lefebvre KA, Tilton SC, Bammler TK, Beyer RP, Srinouanprachan S, Stapleton PL, Farin FM, Gallagher EP (2009) Gene expression profiles in zebrafish brain after acute exposure to domoic acid at symptomatic and asymptomatic doses. Toxicol Sci 107(1):65–77

    CAS  Google Scholar 

  • Lei B, Kang J, Yu Y, Zha J, Li W, Wang Z (2013) β-estradiol 17-valerate affects embryonic development and sexual differentiation in Japanese medaka (Oryzias latipes). Aquat Toxicol 134–135:128–134

    Google Scholar 

  • Lerebours A, Bourdineaud JP, van der Ven K, Vandenbrouck T, Gonzalez P, Camilleri V, Floriani M, Garnier-Laplace J, Adam-Guillermin C (2010) Sublethal effects of waterborne uranium exposures on the zebrafish brain: transcriptional responses and alterations of the olfactory bulb ultrastructure. Environ Sci Technol 44:1438–1443

    CAS  Google Scholar 

  • Li B, Wang C, Ye K, Yu A, Chen Y, Zuo Z (2008) Differential gene expression in the brain of Sebastiscus marmoratus in response to exposure to polychlorinated biphenyls (PCBs). Mar Environ Res 66:548–552

    CAS  Google Scholar 

  • Li X, Liu L, Zhang Y, Fang Q, Li Y, Li Y (2013a) Toxic effects of chlorpyrifos on lysozyme activities, the contents of complement C3 and IgM, and IgM and complement C3 expressions in common carp (Cyprinus carpio L.). Chemosphere 93:428–433

    CAS  Google Scholar 

  • Li X, Ma J, Fang Q, Li Y (2013b) Transcription alterations of microRNAs, cytochrome P4501A1 and 3A65, and AhR and PXR in the liver of zebrafish exposed to crude microcystins. Toxicon 73:17–22

    CAS  Google Scholar 

  • Liang P, Pardee A (1992) Differential display using one base anchor oligo-dT primers. Nucleic Acids Res 22:5763–5764

    Google Scholar 

  • Lie KK, Lanzen A, Breilid H, Olsvik PA (2009) Gene expression profiling in Atlantic cod (Gadus morhua L.) from two contaminated sites using a custom-made cDNA microarray. Environ Toxicol Chem 28:1711–1721

    CAS  Google Scholar 

  • Liu Q, Spitsbergen JM, Cariou R, Huang CY, Jiang N, Goetz G, Hutz RJ, Tonellato PJ, Carvan MJ 3rd (2014) Histopathologic alterations associated with global gene expression due to chronic dietary TCDD exposure in juvenile zebrafish. PLoS One 9(7):e100910

    Google Scholar 

  • Luzio A, Monteiro SM, Fontaínhas-Fernandes AA, Pinto-Carnide O, Matos M, Coimbra AM (2013) Copper induced upregulation of apoptosis related genes in zebrafish (Danio rerio) gill. Aquat Toxicol 128–129:183–189

    Google Scholar 

  • Martinovic D, Villeneuve DL, Kahl MD, Blake LS, Brodin JD, Ankley GT (2009) Hypoxia alters gene expression in the gonads of zebrafish (Danio rerio). Aquat Toxicol 95:258–272

    CAS  Google Scholar 

  • Martyniuk CJ, Gerrie ER, Popesku JT, Ekker M, Trudeau VL (2007) Microarray analysis in the zebrafish (Danio rerio) liver and telencephalon after exposure to low concentration of 17alpha-ethinylestradiol. Aquat Toxicol 84:38–49

    CAS  Google Scholar 

  • Martyniuk CJ, Kroll KJ, Doperalski NJ, Barber DS, Denslow ND (2010) Genomic and proteomic responses to environmentally relevant exposures to dieldrin: indicators of neurodegeneration? Toxicol Sci 117(1):190–199

    CAS  Google Scholar 

  • Matsumoto T, Ishizaki S, Nagashima Y (2011) Differential gene expression profile in the liver of the marine puffer fish Takifugu rubripes induced by intramuscular administration of tetrodotoxin. Toxicon 57:304–310

    CAS  Google Scholar 

  • Mattingly CJ, Colby GT, Forrest JN, Boyer JL (2003) The Comparative Toxicogenomics Database (CTD). Environ Health Perspect 111:793–795

    CAS  Google Scholar 

  • Mellanen P, Soimasuo M, Holmbom B, Oikari A, Santti R (1999) Expression of the vitellogenin gene in the liver of juvenile whitefish (Coregonus lavaretus L. s.l.) exposed to effluents from pulp and paper mills. Ecotoxcol Environ Saf 43:133–137

    CAS  Google Scholar 

  • Meyer JN, Volz DC, Freedman JH, Di Giulio RT (2005) Differential display of hepatic mRNA from killifish (Fundulus heteroclitus) inhabiting a superfund estuary. Aquat Toxicol 73:327–341

    CAS  Google Scholar 

  • Minghetti M, Leaver MJ, Taggart JB, Casadei E, Auslander M, Tom M, George SG (2011) Copper induces Cu-ATPase ATP7A mRNA in a fish cell line, SAF1. Comp Biochem Physiol C Toxicol Pharmacol 154:93–99

    Google Scholar 

  • Moens LN, Soetaert A, van der Ven K, Del-Favero J, De Coen WM (2007a) Use of suppression subtractive hybridization PCR for the development of cDNA arrays for the detection of endocrine disruption in carp (Cyprinus carpio). Comp Biochem Physiol D Genomics Proteomics 2:18–33

    Google Scholar 

  • Moens LN, Smolders R, van der Ven K, van Remortel P, Del-Favero J, DeCoen WM (2007b) Effluent impact assessment using microarray-based analysis in common carp: a systems toxicology approach. Chemosphere 67:2293–2304

    CAS  Google Scholar 

  • Murata K, Sugiyama H, Yasumasu S, Iuchi I, Yasumasu I, Yamagami K (1997) Cloning of cDNA and estrogen induced hepatic gene expression for choriogenin H, a precursor protein of the fish egg envelope (chorion). Proc Natl Acad Sci U S A 94:2050–2055

    CAS  Google Scholar 

  • Nogueira P, Pacheco M, Pereira ML, Mendo S, Rotchell JM (2010) Anchoring novel molecular biomarker responses to traditional responses in fish exposed to environmental contamination. Environ Pollut 158:1783–1790

    CAS  Google Scholar 

  • Olsvik PA, Lindgren M, Maage A (2013) Mercury contamination in deep-water fish: transcriptional responses in tusk (Brosme brosme) from a fjord gradient. Aquat Toxicol 144–145:172–185

    Google Scholar 

  • Oppen-Berntsen DO, Arukwe A, Yadetie F, Lorens JB, Male R (1999) Salmon eggshell protein expression: a marker for environmental estrogens. Mar Biotechnol 1:252–260

    CAS  Google Scholar 

  • Penny C, Adams C (1863) Fourth report, Royal Commission on Pollution of Rivers in Scotland. Vol. 2. Evidence. London (in Jones 1964), pp 377–391

    Google Scholar 

  • Peterson JSK, Bain LJ (2004) Differential gene expression in anthracene-exposed mummichogs (Fundulus heteroclitus). Aquat Toxicol 66:345–355

    CAS  Google Scholar 

  • Peterson RE, Theobald HM, Kimmel GL (1993) Developmental and reproductive toxicity of dioxins and related compounds: cross-species comparisons. Crit Rev Toxicol 23:283–335

    CAS  Google Scholar 

  • Pham CH, Park KS, Kim BC, Kim HN, Gu MB (2011) Construction and characterization of Japanese medaka (Oryzias latipes) hepatic cDNA library and its implementation to biomarker screening in aquatic toxicology. Aquat Toxicol 105:569–575

    CAS  Google Scholar 

  • Pham CH, Yi J, Gu MB (2012) Biomarker gene response in male Medaka (Oryzias latipes) chronically exposed to silver nanoparticle. Ecotoxicol Environ Saf 78:239–245

    CAS  Google Scholar 

  • Pierron F, Normandeau E, Defo MA, Campbell PGC, Bernatchez L, Couture P (2011) Effects of chronic metal exposure on wild fish populations revealed by high-throughput cDNA sequencing. Ecotoxicology 20:1388–1399

    CAS  Google Scholar 

  • Porcher C, Malinge MC, Picat C, Grandchamp B (1992) A simplified method for determination of specific DNA or RNA copy number using quantitative PCR and an automatic DNA sequencer. Biotechniques 13:106–114

    CAS  Google Scholar 

  • Prasch AL, Andreasen EA, Peterson RE, Heideman W (2004) Interactions between 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and hypoxia signaling pathways in zebrafish: hypoxia decreases responses to TCDD in zebrafish embryos. Toxicol Sci 78:68–77

    CAS  Google Scholar 

  • Qu X, Zhang K, Cui Z, Zhang Y, Jiang J, Feng L, Liu Q (2011) Construction and analysis of liver suppression subtractive hybridization library of silver carp (Hypophthalmichthys molitrix) intraperitoneally injected with microcystin-LR. Aquat Toxicol 105:151–156

    CAS  Google Scholar 

  • Ramsay G (1998) DNA chips: state-of-the art. Nat Biotechnol 16:40–44

    CAS  Google Scholar 

  • Rhee J-S, Raisuddin S, Hwang D-S, Lee K-W, Kim IC, Lee J-S (2009) Differential expression of metallothionein (MT) gene by trace metals and endocrine-disrupting chemicals in the hermaphroditic mangrove killifish, Kryptolebias marmoratus. Ecotoxicol Environ Saf 72:206–212

    CAS  Google Scholar 

  • Roling JA, Bain LJ, Baldwin WS (2004) Differential gene expression in mummichogs (Fundulus heteroclitus) following treatment with pyrene: comparison to a creosote contaminated site. Mar Environ Res 57:377–395

    CAS  Google Scholar 

  • Sammar M, Babin PJ, Durliat M, Meiri I, Zchori I, Elizur A, Lubzens E (2001) Retinol binding protein in rainbow trout: molecular properties and mRNA expression in tissues. Gen Comp Endocrinol 123:51–61

    CAS  Google Scholar 

  • Santos D, Matos M, Coimbra AM (2014) Developmental toxicity of endocrine disruptors in early life stages of zebrafish, a genetic and embryogenesis study. Neurotoxicol Teratol 46:18–25

    CAS  Google Scholar 

  • Sasaki YF, Izumiyama F, Nishidate E, Ishibashi S, Tsuda S, Matsusaka N, Asano N, Saotome K, Sofuni T, Hayashi M (1997) Detection of genotoxicity of polluted sea water using shellfish and the alkaline single-cell gel electrophoresis (SCE) assay: a preliminary study. Mutat Res 393:133–139

    CAS  Google Scholar 

  • Sawle AD, Wit E, Whale G, Cossins AR (2010) An information-rich alternative, chemicals testing strategy using a high definition toxicogenomics and zebrafish (Danio rerio) embryos. Toxicol Sci 118(1):128–139

    CAS  Google Scholar 

  • Schiller V, Zhang X, Hecker M, Schäfers C, Fischer R, Fenske M (2014) Species-specific considerations in using the fish embryo test as an alternative to identify endocrine disruption. Aquat Toxicol 155:62–72

    CAS  Google Scholar 

  • Sheader DL, Williams TD, Lyons BP, Chipman JK (2006) Oxidative stress response of European flounder (Platichthys flesus) to cadmium determined by a custom cDNA microarray. Mar Environ Res 62:33–44

    CAS  Google Scholar 

  • Shumway M, Cochrane G, Sugawara H (2010) Archiving next generation sequencing data. Nucleic Acids Res 38:D870–D871

    CAS  Google Scholar 

  • Song Y, Salbu B, Heier LS, Teien HC, Lind OC, Oughton D, Petersen K, Rosseland BO, Skipperud L, Tollefsen KE (2012) Early stress responses in Atlantic salmon (Salmo salar) exposed to environmentally relevant concentrations of uranium. Aquat Toxicol 112:62–71

    Google Scholar 

  • Song Y, Salbu B, Teien H-C, Heier LS, Rosseland BO, Høgåsen T, Tollefsen KE (2014) Hepatic transcriptomic profiling reveals early toxicological mechanisms of uranium in Atlantic salmon (Salmo salar). BMC Genomics 15:694

    Google Scholar 

  • Sumpter JP, Jobling S (1995) Vitellogenin as a biomarker for estrogenic contamination of the aquatic environment. Environ Health Perspect 103:173–178

    CAS  Google Scholar 

  • Sun S, Ge X, Zhu J, Xuan F, Jiang X (2014) Identification and mRNA expression of antioxidant enzyme genes associated with the oxidative stress response in the Wuchang bream (Megalobrama amblycephala Yih) in response to acute nitrite exposure. Comp Biochem Physiol C Toxicol Pharmacol 159:69–77

    CAS  Google Scholar 

  • Tian H, Wu P, Wang W, Ru S (2015) Disruptions in aromatase expression in the brain, reproductive behavior, and secondary sexual characteristics in male guppies (Poecilia reticulata) induced by tributyltin. Aquat Toxicol 162:117–125

    CAS  Google Scholar 

  • Tilton SC, Gerwick LG, Hendricks JD et al (2005) Use of a rainbow trout oligonucleotide microarray to determine transcriptional patterns in aflatoxin B1-induced hepatocellular carcinoma compared to adjacent liver. Toxicol Sci 88(2):319–330

    CAS  Google Scholar 

  • Turner C, Sawle A, Fenske M, Cossins A (2012) Implications of the solvent vehicles dimethylformamide and dimethylsulfoxide for establishing transcriptomic endpoints in the zebrafish embryo toxicity test. Environ Toxicol Chem 31:593–604

    CAS  Google Scholar 

  • van Boxtel AL, Pieterse B, Cenijn P, Kamstra JH, Brouwer A, van Wieringen W, de Boer J, Legler J (2010) Dithiocarbamates induce craniofacial abnormalities and downregulate sox9a during zebrafish development. Toxicol Sci 117(1):209–217

    Google Scholar 

  • Varela-Valencia R, Gomez-Ortiz N, Oskam G, de Coss R, Rubio-Pina J, Rio-Garcıa M, Albores-Medina A, Zapata-Perez O (2014) The effect of titanium dioxide nanoparticles on antioxidant gene expression in tilapia (Oreochromis niloticus). J Nanopart Res 16:2369

    Google Scholar 

  • Volz DC, Bencic DC, Hinton DE, Law JM, Kullman SW (2005) 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) induces organ-specific differential gene expression in male Japanese medaka (Oryzias latipes). Toxicol Sci 85:572–584

    CAS  Google Scholar 

  • Volz DC, Hinton DE, Law JM, Kullman SW (2006) Dynamic gene expression changes precede dioxin-induced liver pathogenesis in medaka fish. Toxicol Sci 89(2):524–534

    CAS  Google Scholar 

  • Wang Z, Gerstein M, Snyder M (2009) RNA-Seq: a revolutionary tool for transcriptomics. Nat Rev Genet 10(1):57–63

    CAS  Google Scholar 

  • Wang R-L, Bencic D, Lazorchak J, Villeneuve D, Ankley GT (2011) Transcriptional regulatory dynamics of the hypothalamic–pituitary–gonadal axis and its peripheral pathways as impacted by the 3-beta HSD inhibitor trilostane in zebrafish (Danio rerio). Ecotoxicol Environ Saf 74:1461–1470

    CAS  Google Scholar 

  • Wang Q, Lam JCW, Han J, Wang X, Guo Y, Lam PKS, Zhou B (2015a) Developmental exposure to the organophosphorus flame retardant tris (1,3-dichloro-2-propyl) phosphate: estrogenic activity, endocrine disruption and reproductive effects on zebrafish. Aquat Toxicol 160:163–171

    CAS  Google Scholar 

  • Wang Z, Zhang X, Tian H, Wang W, Ru S (2015b) Effects of monocrotophos pesticide on steroidogenesis and transcription of steroidogenic enzymes in rainbow trout RTG-2 cells involving the protein kinase A signal pathway. Toxicol In Vitro 29:155–161

    Google Scholar 

  • Waring JF, Ciurlionis R, Jolly RA, Heindel M, Ulrich RG (2001) Microarray analysis of hepatotoxins in vitro reveals a correlation between gene expression profiles and mechanisms of toxicity. Toxicol Lett 120:359–368

    CAS  Google Scholar 

  • Webster TMU, Bury N, van Aerle R, Santos EM (2013) Global transcriptome profiling reveals molecular mechanisms of metal tolerance in a chronically exposed wild population of brown trout. Environ Sci Technol 47:8869–8877

    Google Scholar 

  • Wei Y, Liu Y, Wang J, Tao Y, Dai J (2008) Toxicogenomic analysis of the hepatic effects of perfluorooctanoic acid on rare minnows (Gobiocypris rarus). Toxicol Appl Pharmacol 226:285–297

    CAS  Google Scholar 

  • Wei Y, Shi X, Zhang H, Wang J, Zhou B, Dai J (2009) Combined effects of polyfluorinated and perfluorinated compounds on primary cultured hepatocytes from rare minnow (Gobiocypris rarus) using toxicogenomic analysis. Aquat Toxicol 95:27–36

    CAS  Google Scholar 

  • Williams DE, Lech JJ, Buhler DR (1998) Xenobiotics and xenoestrogens in fish: modulation of cytochrome P450 and carcinogenesis. Mutat Res 399:179–192

    CAS  Google Scholar 

  • Williams TD, Gensberg K, Minchin SD, Chipman JK (2003) A DNA expression array to detect toxic stress response in European flounder (Platichthys flesus). Aquat Toxicol 65:141–157

    CAS  Google Scholar 

  • Williams TD, Diab AM, Gubbins M, Collins C, Matejusova I, Kerr R, Chipman JK, Kuiper R, Vethaak AD, George SG (2013) Transcriptomic responses of European flounder (Platichthys flesus) liver to a brominated flame retardant mixture. Aquat Toxicol 142–143:45–52

    Google Scholar 

  • Williams TD, Mirbahai L, Chipman JK (2014) The toxicological application of transcriptomics and epigenomics in zebrafish and other teleosts. Brief Funct Genomics 13(2):157–171

    Google Scholar 

  • Wintz H, Yoo LJ, Loguinov A, Wu YY, Steevens JA, Holland RD, Beger RD, Perkins EJ, Hughes O, Vulpe CD (2006) Gene expression profiles in fathead minnow exposed to 2,4-DNT: correlation with toxicity in mammals. Toxicol Sci 94(1):71–82

    CAS  Google Scholar 

  • Wiseman SB, He Y, Din MG, Martin JW, Jones PD, Hecker M, John P, Giesy JP (2013) Transcriptional responses of male fathead minnows exposed to oil sands process-affected water. Comp Biochem Physiol C Toxicol Pharmacol 157:227–235

    CAS  Google Scholar 

  • Woo S, Won H, Lee A, Yum S (2012) Oxidative stress and gene expression in diverse tissues of Oryzias javanicus exposed to 17β-estradiol. Mol Cell Toxicol 8:263–269

    CAS  Google Scholar 

  • Woźny M, Brzuzan P, Wolińska L, Góra M, Łuczyński MK (2012) Differential gene expression in rainbow trout (Oncorhynchus mykiss) liver and ovary after exposure to zearalenone. Comp Biochem Physiol C Toxicol Pharmacol 156:221–228

    Google Scholar 

  • Wu M, Zuo Z, Li B, Huang L, Chen M, Wang C (2013) Effects of low-level hexabromocyclododecane (HBCD) exposure on cardiac development in zebrafish embryos. Ecotoxicology 22:1200–1207

    CAS  Google Scholar 

  • Xing H, Han Y, Li S, Wang J, Wang X, Xu S (2010) Alterations in mRNA expression of acetylcholinesterase in brain and muscle of common carp exposed to atrazine and chlorpyrifos. Ecotoxicol Environ Saf 73:1666–1670

    CAS  Google Scholar 

  • Yadetie F, Male R (2002) Effects of 4-nonylphenol on gene expression of pituitary hormones in juvenile Atlantic salmon (Salmo salar). Aquat Toxicol 58:113–129

    CAS  Google Scholar 

  • Yadetie F, Karlsen OA, Lanzén A, Berg K, Olsvik P, Hogstrand C, Goksøyr A (2013) Global transcriptome analysis of Atlantic cod (Gadus morhua) liver after in vivo methylmercury exposure suggests effects on energy metabolism pathways. Aquat Toxicol 126:314–325

    CAS  Google Scholar 

  • Yamauchi M, Kim E-Y, Iwata H, Shima Y, Tanabe S (2006) Toxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in developing red seabream (Pagrus major) embryo: an association of morphological deformities with AHR1, AHR2 and CYP1A expressions. Aquat Toxicol 80:166–179

    CAS  Google Scholar 

  • Yamauchi R, Ishibashi H, Hirano M, Mori T, Kim J-W, Arizono K (2008) Effects of synthetic polycyclic musks on estrogen receptor, vitellogenin, pregnane X receptor, and cytochrome P450 3A gene expression in the livers of male medaka (Oryzias latipes). Aquat Toxicol 90:261–268

    CAS  Google Scholar 

  • Yan W, Zhou Y, Yang J, Li S, Hu D, Wang J, Chen J, Li G (2012) Waterborne exposure to microcystin-LR alters thyroid hormone levels and gene transcription in the hypothalamic–pituitary–thyroid axis in zebrafish larvae. Chemosphere 87:1301–1307

    CAS  Google Scholar 

  • Yang S, Liu S, Ren Z, Jiao X, Qin S (2014) Induction of oxidative stress and related transcriptional effects of perfluorononanoic acid using an in vivo assessment. Comp Biochem Physiol C Toxicol Pharmacol 160:60–65

    CAS  Google Scholar 

  • Yuan L, Lv B, Zha J, Wang W, Wang Z (2014) Basal and benzo[a]pyrene-induced expression profile of phase I and II enzymes and ABC transporter mRNA in the early life stage of Chinese rare minnows (Gobiocypris rarus). Ecotoxicol Environ Saf 106:86–94

    CAS  Google Scholar 

  • Zanette J, Jenny MJ, Goldstone JV, Woodin BR, Watka LA, Bainy ACD, Stegeman JJ (2009) New cytochrome P450 1B1, 1C2 and 1D1 genes in the killifish Fundulus heteroclitus: basal expression and response of five killifish CYP1s to the AHR agonist PCB126. Aquat Toxicol 93:234–243

    CAS  Google Scholar 

  • Zhang X, Gao L, Yang K, Tian H, Wang W, Ru S (2013) Monocrotophos pesticide modulates the expression of sexual differentiation genes and causes phenotypic feminization in zebrafish (Danio rerio). Comp Biochem Physiol C Toxicol Pharmacol 157:33–40

    CAS  Google Scholar 

Download references

Acknowledgment

The study was financially supported by the Ministry of Education, Youth and Sports of the Czech Republic—projects “CENAKVA” (No. CZ.1.05/2.1.00/01.0024) and “CENAKVA II” (No. LO1205 under the NPU I program).

Author information

Authors and Affiliations

  1. Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Zátiší 728/II, 389 25, Vodňany, Czech Republic

    Girish Kumar

  2. Department of Physiological Sciences and Center for Environmental and Human Toxicology, University of Florida, Gainesville, FL, 32611, USA

    Nancy D. Denslow

Authors
  1. Girish Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nancy D. Denslow
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Girish Kumar .

Editor information

Editors and Affiliations

  1. University of Amsterdam, Amsterdam, The Netherlands

    Pim de Voogt

Additional information

Conflict of Interest

The authors declare that they have no conflict of interest.

Rights and permissions

Reprints and permissions

Copyright information

© 2016 Springer International Publishing Switzerland

About this chapter

Cite this chapter

Kumar, G., Denslow, N.D. (2016). Gene Expression Profiling in Fish Toxicology: A Review. In: de Voogt, P. (eds) Reviews of Environmental Contamination and Toxicology Volume 241. Reviews of Environmental Contamination and Toxicology, vol 241. Springer, Cham. https://doi.org/10.1007/398_2016_10

Download citation

Publish with us

Policies and ethics

Search

Navigation