Skip to main content

Fluorescence Techniques in Non-Viral Gene Therapy

  • Chapter
Fluorescence Spectroscopy in Biology

Part of the book series: Springer Series on Fluorescence ((SS FLUOR,volume 3))

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Brooks G (2002) Gene therapy: the use of DNA as a drug. Pharmaceutical Press, London

    Google Scholar 

  2. Wiley (2003) http://www.wiley.com/legacy/wileychi/genmed/clinical/

    Google Scholar 

  3. Blagbrough IS, Geall AJ, Neal AP (2003) Polyamines and novel polyamine conjugates interact with DNA in ways that can be exploited in non-viral gene therapy. Biochem Soc Trans 31:397–406

    Article  CAS  Google Scholar 

  4. Kumar VV, Singh RS, Chaudhuri A (2003) Cationic transfection lipids in gene therapy: successes, set-backs, challenges and promises. Curr Med Chem 10:1297–1306

    CAS  Google Scholar 

  5. Schmidt-Wolf GD, Schmidt-Wolf IGH (2003) Non-viral and hybrid vectors in human gene therapy: an update. Trends Mol Med 9:67–72

    Article  CAS  Google Scholar 

  6. Liu F, Huang L (2002) Development of non-viral vectors for systemic gene delivery. J Control Release 78:259–266

    Article  CAS  Google Scholar 

  7. Godbey WT, Mikos AG (2001) Recent progress in gene delivery using non-viral transfer complexes. J Control Release 72:115–125

    Article  CAS  Google Scholar 

  8. Li S, Huang L (2000) Non-viral gene therapy: promises and challenges. Gene Ther 7:31–34

    CAS  Google Scholar 

  9. Lollo CP, Banaszczyk MG, Chiou HC (2000) Obstacles and advances in non-viral gene delivery. Curr Opin Mol Ther 2:136–142

    CAS  Google Scholar 

  10. MacLachlan I, Cullis P, Graham RW (1999) Progress towards a synthetic virus for systemic gene therapy. Curr Opin Mol Ther 1:252–259

    CAS  Google Scholar 

  11. Felgner PL, Barenholz Y, Behr JP, Cheng SH, Cullis P, Huang L, Jessee JA, Seymour L, Szoka F, Thierry AR, Wagner E, Wu G (1997) Nomenclature for synthetic gene delivery systems. Hum Gene Ther 8:511–512

    CAS  Google Scholar 

  12. El Aneed A (2004) An overview of current delivery systems in cancer gene therapy. J Control Release 94:1–14

    Google Scholar 

  13. Roux D, Cheneviera P, Pott T, Navailles L, Regev O, Monval OM (2004) Conception and realization of a non-cationic non-viral DNA vector. Curr Med Chem 11:169–177

    Article  CAS  Google Scholar 

  14. Russell SJ (2003) Rise of the nanomachines. Nat Biotechnol 21:872–873

    Article  CAS  Google Scholar 

  15. Kamiya H, Akita H, Harashima H (2003) Pharmacokinetic and pharmacodynamic considerations in gene therapy. Drug Discov Today 8:990–996

    Article  CAS  Google Scholar 

  16. Ruponen M, Honkakoski P, Ronkko S, Pelkonen J, Tammi M, Urtti A (2003) Extracellular and intracellular barriers in non-viral gene delivery. J Control Release 93:213–217

    Article  CAS  Google Scholar 

  17. Wiethoff CM, Middaugh CR (2003) Barriers to nonviral gene delivery. J Pharm Sci 92: 203–217

    CAS  Google Scholar 

  18. Ferrari S, Geddes DM, Alton EWFW (2002) Barriers to and new approaches for gene therapy and gene delivery in cystic fibrosis. Adv Drug Deliv Rev 54:1373–1393

    Article  CAS  Google Scholar 

  19. Vijayanathan V, Thomas T, Thomas TJ (2002) DNA nanoparticles and development of DNA delivery vehicles for gene therapy. Biochemistry 41:14085–14094

    Article  CAS  Google Scholar 

  20. Vijayanathan V, Thomas T, Shirahata A, Thomas TJ (2001) DNA condensation by polyamines: a laser light scattering study of structural effects. Biochemistry 40:13644–13651

    Article  CAS  Google Scholar 

  21. Hud NV, Plavec J (2003) A unified model for the origin of DNA sequence-directed curvature. Biopolymers 69:144–159

    Article  CAS  Google Scholar 

  22. Golan R, Pietrasanta LI, Hsieh W, Hansma HG (1999) DNA toroids: stages in condensation. Biochemistry 38:14069–14076

    Article  CAS  Google Scholar 

  23. Li S, Tseng WC, Stolz DB, Wu SP, Watkins SC, Huang L (1999) Dynamic changes in the characteristics of cationic lipidic vectors after exposure to mouse serum: implications for intravenous lipofection. Gene Ther 6:585–594

    CAS  Google Scholar 

  24. Oupicky D, Konak C, Dash PR, Seymour LW, Ulbrich K (1999) Effect of albumin and polyanion on the structure of DNA complexes with polycation containing hydrophilic nonionic block. Bioconjug Chem 10:764–772

    Article  CAS  Google Scholar 

  25. Welz C, Fahr A (2001) Spectroscopic methods for characterization of nonviral gene delivery systems from a pharmaceutical point of view. Appl Spectrosc Rev 36:333–397

    CAS  Google Scholar 

  26. Geall AJ, Blagbrough IS (2000) Rapid and sensitive ethidium bromide fluorescence quenching assay of polyamine conjugate-DNA interactions for the analysis of lipoplex formation in gene therapy. J Pharm Biomed Anal 22:849–859

    Article  CAS  Google Scholar 

  27. Gershon H, Ghirlando R, Guttman SB, Minsky A (1993) Mode of formation and structural features of DNA-cationic liposome complexes used for transfection. Biochemistry 32: 7143–7151

    Article  CAS  Google Scholar 

  28. Corsi K, Chellat F, Yahia L, Fernandes JC (2003) Mesenchymal stem cells, MG63 and HEK293 transfection using chitosan-DNA nanoparticles. Biomaterials 24:1255–1264

    Article  CAS  Google Scholar 

  29. Read ML, Etrych T, Ulbrich K, Seymour LW (1999) Characterisation of the binding interaction between poly(L-lysine) and DNA using the fluorescamine assay in the preparation of non-viral gene delivery vectors. FEBS Lett 461:96–100

    Article  CAS  Google Scholar 

  30. Bode J, Willmitzer L (1975) Application of fluorescamine to the study of protein-DNA interactions. Nucleic Acids Res 2:1951–1965

    CAS  Google Scholar 

  31. Cosa G, Focsaneanu KS, Mclean JRN, McNamee JP, Scaiano JC (2001) Photophysical properties of fluorescent DNA-dyes bound to single-and double-stranded DNA in aqueous buffered solution. Photochem Photobiol 73:585–599

    Article  CAS  Google Scholar 

  32. Singer VL, Jones LJ, Yue ST, Haugland RP (1997) Characterization of PicoGreen reagent and development of a fluorescence-based solution assay for double-stranded DNA quantitation. Anal Biochem 249:228–238

    Article  CAS  Google Scholar 

  33. Leong KW, Mao HQ, Truong L, Roy K, Walsh SM, August JT (1998) DNA-polycation nanospheres as non-viral gene delivery vehicles. J Control Release 53:183–193

    Article  CAS  Google Scholar 

  34. Geall AJ, Taylor RJ, Earll ME, Eaton MAW, Blagbrough IS (2000) Synthesis of cholesteryl polyamine carbamates: pKa studies and condensation of calf thymus DNA. Bioconjug Chem 11:314–326

    Article  CAS  Google Scholar 

  35. Wilson RW, Bloomfield VA (1979) Counterion-induced condensation of deoxyribonucleic acid. A light-scattering study. Biochemistry 18:2192–2196

    CAS  Google Scholar 

  36. Neal AP, Blagbrough IS (2001) Design and synthesis of fluorescent cholesterol and lithocholic acid polyamine conjugates. Abstr Pap Am Chem Soc 221:332-MEDI

    Google Scholar 

  37. Neal AP, Blagbrough IS (2001) Fluorescent steroidal lipopolyamine conjugates for monitoring gene delivery. Abstr Pap Am Chem Soc 221:352-MEDI

    Google Scholar 

  38. Kral T, Hof M, Langner M (2002) The effect of spermine on plasmid condensation and dye release observed by fluorescence correlation spectroscopy. Biol Chem 383:331–335

    Article  CAS  Google Scholar 

  39. Kral T, Hof M, Jurkiewicz, P, Langner M (2002) Fluorescence correlation spectroscopy (FCS) as a tool to study DNA condensation with hexadecyltrimethylammonium bromide (HTAB). Cell Mol Biol Lett 7:203–211

    CAS  Google Scholar 

  40. Kral T, Langner M, Benes M, Baczynska D, Ugorski M, Hof M (2002) The application of fluorescence correlation spectroscopy in detecting DNA condensation. Biophys Chem 95:135–144

    Article  CAS  Google Scholar 

  41. Itaka K, Harada A, Nakamura K, Kawaguchi H, Kataoka K (2002) Evaluation by fluorescence resonance energy transfer of the stability of nonviral gene delivery vectors under physiological conditions. Biomacromolecules 3:841–845

    Article  CAS  Google Scholar 

  42. Zelphati O, Szoka FC Jr (1996) Mechanism of oligonucleotide release from cationic liposomes. Proc Natl Acad Sci USA 93:11493–11498

    Article  CAS  Google Scholar 

  43. Godbey WT, Wu KK, Mikos AG (1999) Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery. Proc Natl Acad Sci USA 96:5177–5181

    Article  CAS  Google Scholar 

  44. Byk G, Dubertret C, Escriou V, Frederic M, Jaslin G, Rangara R, Pitard B, Crouzet J, Wils P, Schwartz B, Scherman D (1998) Synthesis, activity, and structure-activity relationship studies of novel cationic lipids for DNA transfer. J Med Chem 41:224–235

    Article  Google Scholar 

  45. Byk G, Scherman D (2000) Genetic chemistry: tools for gene therapy coming from unexpected directions. Drug Dev Res 50:566–572

    Article  CAS  Google Scholar 

  46. Byk G, Wetzer B, Frederic M, Dubertret C, Pitard B, Jaslin G, Scherman D (2000) Reductionsensitive lipopolyamines as a novel nonviral gene delivery system for modulated release of DNA with improved transgene expression. J Med Chem 43:4377–4387

    Article  CAS  Google Scholar 

  47. Uyechi LS, Gagne L, Thurston G, Szoka FC (2001) Mechanism of lipoplex gene delivery in mouse lung: binding and internalization of fluorescent lipid and DNA components. Gene Ther 8:828–836

    Article  CAS  Google Scholar 

  48. Ishii T, Okahata Y, Sato T (2001) Mechanism of cell transfection with plasmid/chitosan complexes. Biochim Biophys Acta Biomembr 1514:51–64

    CAS  Google Scholar 

  49. Naylor BL, Picardo M, Homan R, Pownall HJ (1991) Effects of fluorophore structure and hydrophobicity on the uptake and metabolism of fluorescent lipid analogs. Chem Phys Lipids 58:111–119

    CAS  Google Scholar 

  50. Bolton PH, Kearns DR (1978) Spectroscopic properties of ethidium monoazide: a fluorescent photoaffinity label for nucleic acids. Nucleic Acids Res 5:4891–4903

    Article  CAS  Google Scholar 

  51. Cantrell CE, Yielding KL, Pruitt KM (1979) Efficiency of photolytic binding of ethidium monoazide to nucleic acids and synthetic polynucleotides. Mol Pharmacol 15:322–330

    CAS  Google Scholar 

  52. Zabner J, Fasbender AJ, Moninger T, Poellinger KA, Welsh MJ (1995) Cellular and molecular barriers to gene transfer by a cationic lipid. J Biol Chem 270:18997–19007

    CAS  Google Scholar 

  53. Neves C, Byk G, Escriou V, Bussone F, Scherman D, Wils P (2000) Novel method for covalent fluorescent labeling of plasmid DNA that maintains structural integrity of the plasmid. Bioconjug Chem 11:51–55

    Article  CAS  Google Scholar 

  54. Keller GH, Huang DP, Manak MM (1989) Labeling of DNA probes with a photoactivatable hapten. Anal Biochem 177:392–395

    CAS  Google Scholar 

  55. Slattum PS, Loomis AG, Machnik KJ, Watt MA, Duzeski JL, Budker VG, Wolff JA, Hagstrom JE (2003) Efficient in vitro and in vivo expression of covalently modified plasmid DNA. Mol Ther 8:255–263

    Article  CAS  Google Scholar 

  56. Yoshinaga T, Yasuda K, Ogawa Y, Takakura Y (2002) Efficient uptake and rapid degradation of plasmid DNA by murine dendritic cells via a specific mechanism. Biochem Biophys Res Commun 299:389–394

    Article  CAS  Google Scholar 

  57. Byrnes CK, Nass PH, Shim J, Duncan MD, Lacy B, Harmon JW (2002) Novel nuclear shuttle peptide to increase transfection efficiency in esophageal mucosal cells. J Gastrointest Surg 6:37–42

    Article  Google Scholar 

  58. Murata S, Herman P, Lin HJ, Lakowicz JR (2000) Fluorescence lifetime imaging of nuclear DNA: effect of fluorescence resonance energy transfer. Cytometry 41:178–185

    Article  CAS  Google Scholar 

  59. Murata S, Herman P, Mochizuki K, Nakazawa T, Kondo T, Nakamura N, Lakowicz JR, Katoh R (2003) Spatial distribution analysis of AT-and GC-rich regions in nuclei using corrected fluorescence resonance energy transfer. J Histochem Cytochem 51:951–958

    CAS  Google Scholar 

  60. Murata S, Herman P, Lakowicz JR (2001) Texture analysis of fluorescence lifetime images of AT-and GC-rich regions in nuclei. J Histochem Cytochem 49:1443–1451

    CAS  Google Scholar 

  61. Murata S, Herman P, Lakowicz JR (2001) Texture analysis of fluorescence lifetime images of nuclear DNA with effect of fluorescence resonance energy transfer. Cytometry 43:94–100

    Article  CAS  Google Scholar 

  62. Murata S, Kusba J, Piszczek G, Gryczynski I, Lakowicz JR (2000) Donor fluorescence decay analysis for energy transfer in double-helical DNA with various acceptor concentrations. Biopolymers 57:306–315

    Article  CAS  Google Scholar 

  63. Malicka J, Gryczynski I, Maliwal BP, Fang JY, Lakowicz JR (2003) Fluorescence spectral properties of cyanine dye labeled DNA near metallic silver particles. Biopolymers 72:96–104

    Article  CAS  Google Scholar 

  64. Kang JS, Abugo OO, Lakowicz JR (2002) Dynamics of supercoiled and relaxed pTZ18U plasmids probed with a long-lifetime metal-ligand complex. J Biochem Mol Biol 35:389–394

    CAS  Google Scholar 

  65. Kang JS, Abugo OO, Lakowicz JR (2002) Dynamics of supercoiled and linear pTZ18U plasmids observed with a long-lifetime metal-ligand complex. Biopolymers 67:121–128

    Article  CAS  Google Scholar 

  66. Cormack BP, Valdivia RH, Falkow S (1996) FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173:33–38

    Article  CAS  Google Scholar 

  67. Deluca M, McElory WD (1978) Purification and properties of firefly luciferase. Meth Enzymol 57:3–15

    CAS  Google Scholar 

  68. Vile GF, Tyrrell RM (1993) Oxidative stress resulting from ultraviolet-A irradiation of human skin fibroblasts leads to a heme oxygenase-dependent increase in ferritin. J Biol Chem 268:14678–14681

    CAS  Google Scholar 

  69. Zelphati O, Liang XW, Hobart P, Felgner PL (1999) Gene chemistry: functionally and conformationally intact fluorescent plasmid DNA. Hum Gene Ther 10:15–24

    Article  CAS  Google Scholar 

  70. Hyvonen Z, Plotniece A, Reine I, Chekavichus B, Duburs G, Urtti A (2000) Novel cationic amphiphilic 1,4-dihydropyridine derivatives for DNA delivery. Biochim Biophys Acta 1509:51–466

    Google Scholar 

  71. Hyvonen Z, Ruponen M, Ronkko S, Suhonen P, Urtti A (2002) Cellular and intracellular factors influencing gene transfection mediated by 1,4-dihydropyridine amphiphiles. Eur J Pharm Sci 15:449–460

    Article  CAS  Google Scholar 

  72. Serikawa T, Suzuki N, Kikuchi H, Tanaka K, Kitagawa T (2000) A new cationic liposome for efficient gene delivery with serum into cultured human cells: a quantitative analysis using two independent fluorescent probes. Biochim Biophys Acta Biomembr 1467:419–430

    CAS  Google Scholar 

  73. Collins L, Sawyer GJ, Zhang XH, Gustafsson K, Fabre JW (2000) In vitro investigation of factors important for the delivery of an integrin-targeted nonviral DNA vector in organ transplantation. Transplantation 69:1168–1176

    CAS  Google Scholar 

  74. Krichevsky O, Bonnet G (2002) Fluorescence correlation spectroscopy: the technique and its applications. Rep Progr Phys 65:251–297

    CAS  Google Scholar 

  75. Clamme JP, Azoulay J, Mely Y (2003) Monitoring of the formation and dissociation of polyethylenimine/DNA complexes by two-photon fluorescence correlation spectroscopy. Biophys J 84:1960–1968

    CAS  Google Scholar 

  76. Van Rompaey E, Engelborghs Y, Sanders N, De Smedt SC, Demeester J (2001) Interactions between oligonucleotides and cationic polymers investigated by fluorescence correlation spectroscopy. Pharm Res 18:928–936

    Google Scholar 

  77. Jurkiewicz P, Okruszek A, Hof M, Langner M (2003) Associating oligonucleotides with positively charged liposomes. Cell Mol Biol Lett 8:77–84

    CAS  Google Scholar 

  78. Clamme JP, Krishnamoorthy G, Mely Y (2003) Intracellular dynamics of the gene delivery vehicle polyethylenimine during transfection: investigation by two-photon fluorescence correlation spectroscopy. Biochim Biophys Acta Biomembr 1617:52–61

    CAS  Google Scholar 

  79. Wiethoff CM, Gill ML, Koe GS, Koe JG, Middaugh CR (2002) The structural organization of cationic lipid-DNA complexes. J Biol Chem 277:44980–44987

    Article  CAS  Google Scholar 

  80. Zhang Y, Garzon-Rodriguez W, Manning MC, Anchordoquy TJ (2003) The use of fluorescence resonance energy transfer to monitor dynamic changes of lipid-DNA interactions during lipoplex formation. Biochim Biophys Acta Biomembr 1614:182–192

    CAS  Google Scholar 

  81. Itaka K, Harada A, Yamasaki Y, Nakamura K, Kawaguchi H, Kataoka K (2004) In situ single cell observation by fluorescence resonance energy transfer reveals fast intra-cytoplasmic delivery and easy release of plasmid DNA complexed with linear polyethylenimine. J Gene Med 6:76–84

    Article  CAS  Google Scholar 

  82. Pal R, Barenholz Y, Wagner RR (1988) Pyrene phospholipid as a biological fluorescent probe for studying fusion of virus membrane with liposomes. Biochemistry 27:30–36

    Article  CAS  Google Scholar 

  83. Struck DK, Hoekstra D, Pagano RE (1981) Use of resonance energy transfer to monitor membrane fusion. Biochemistry 20:4093–4099

    Article  CAS  Google Scholar 

  84. Remy-Kristensen A, Clamme JP, Vuilleumier C, Kuhry JG, Mely Y (2001) Role of endocytosis in the transfection of L929 fibroblasts by polyethylenimine/DNA complexes. Biochim Biophys Acta 1514:21–32

    CAS  Google Scholar 

  85. Ira A, Mely Y, Krishnamoorthy G (2003) DNA vector polyethylenimine affects cell pH and membrane potential: a time-resolved fluorescence microscopy study. J Fluoresc 13:339–347

    CAS  Google Scholar 

  86. Lleres D, Dauty E, Behr JP, Mely Y, Duportail G (2001) DNA condensation by an oxidizable cationic detergent. Interactions with lipid vesicles. Chem Phys Lipids 111:59–71

    Article  CAS  Google Scholar 

  87. Bacia K, Schwille P (2003) A dynamic view of cellular processes by in vivo fluorescence auto-and cross-correlation spectroscopy. Methods 29:74–85

    Article  CAS  Google Scholar 

  88. Haustein E, Schwille P (2003) Ultrasensitive investigations of biological systems by fluorescence correlation spectroscopy. Methods 29:153–166

    Article  CAS  Google Scholar 

  89. Widengren J, Schweinberger E, Berger S, Seidel CAM (2001) Two new concepts to measure fluorescence resonance energy transfer via fluorescence correlation spectroscopy: theory and experimental realizations. J Phys Chem A 105:6851–6866

    Article  CAS  Google Scholar 

  90. Lucas B, Van Rompaey E, De Smedt SC, Demeester J, Van Oostveldt P (2002) Dual-color fluorescence fluctuation spectroscopy to study the complexation between poly-L-lysine and oligonucleotides. Macromolecules 35:8152–8160

    Article  CAS  Google Scholar 

  91. Weiss S (1999) Fluorescence spectroscopy of single biomolecules. Science 283:1676–1683

    Article  CAS  Google Scholar 

  92. Sako Y, Yanagida T (2003) Single-molecule visualization in cell biology. Nat Cell Biol SS1–SS5

    Google Scholar 

  93. Su TJ, Theofanidou E, Arlt J, Dryden DTF, Crain J (2004) Single molecule fluorescence imaging and its application to the study of DNA condensation. J Fluoresc 14:65–69

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2005 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Adjimatera, N., Neal, A.P., Blagbrough, I.S. (2005). Fluorescence Techniques in Non-Viral Gene Therapy. In: Hof, M., Hutterer, R., Fidler, V. (eds) Fluorescence Spectroscopy in Biology. Springer Series on Fluorescence, vol 3. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-27004-3_12

Download citation

Publish with us

Policies and ethics