Skip to main content

Using Antibody Arrays to Detect Protein-Protein Interactions

  • Chapter

This is a preview of subscription content, log in via an institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD   169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Alberts, B. (1998). The Cell as a Collection of Protein Machines: Preparing the Next Generation of Molecular Biologists. Cell 92(3): 291–4

    Article  CAS  PubMed  Google Scholar 

  • Duffy, H.S., Ashton, A. W., O’Donnell, P, Coombs, W., Taffet, S. M., Delmar, M. and Spray, D.C. (2004) Regulation of Connexin43 Protein Complexes by Intracellular Acidification. Circ Res 94: 215–222

    Article  CAS  PubMed  Google Scholar 

  • Duffy, H.S., Delmar, M., Coombs, M., Taffet, S.M., Hertzberg, E.L. and Spray, D.C. (2001). Functional Demonstration of Connexin-Protein Binding Using Surface Plasmon Resonance. Cell Commun Adhes. 8(4–6): 225–9

    CAS  PubMed  Google Scholar 

  • Ekins, R. P. (1998). Ligand Assays: From Electrophoresis to Miniaturized Microarrays. Clin Chem 44(9): 2015–30

    CAS  PubMed  Google Scholar 

  • Ge, H. (2000). UPA, A Universal Protein Array System for Quantitative Detection of Protein-Protein, Protein-DNA, Protein-RNA and Protein-Ligand Interactions. Nucleic Acids Res 28(2): e3

    Article  CAS  PubMed  Google Scholar 

  • Giepmans, B. N. and Moolenaar, W. H. (1998). The Gap Junction Protein Connexin43 Interacts with the Second PDZ Domain of the Zona Occludens-1 protein. Curr Biol 8(16): 931–4

    Article  CAS  PubMed  Google Scholar 

  • Hsu, Y. T. and Youle, R. J. (1998). Bax in Murine Thymus is a Soluble Monomeric Protein that Displays Differential Detergent-Induced Conformations. J Biol Chem 273(17): 10777–83

    Article  CAS  PubMed  Google Scholar 

  • Kukar, T., Eckenrode, S., Gu, Y., Lian, W., Megginson, M., She, J. X. and Wu, D. (2002). Protein Microarrays to Detect Protein-Protein Interactions Using Red and Green Fluorescent Proteins. Anal Biochem 306(1): 50–4

    Article  CAS  PubMed  Google Scholar 

  • Kusnezow, W., Jacob, A., Walijew, A., Diehl, F. and Hoheisel, J. D. (2003). Antibody Microarrays: An Evaluation of Production Parameters. Proteomics 3(3): 254–64

    Article  CAS  PubMed  Google Scholar 

  • Lueking, A., Horn, M., Eickhoff, H., Bussow, K., Lehrach, H. and Walter, G. (1999). Protein Microarrays for Gene Expression and Antibody Screening. Anal Biochem 270: 103–111

    Article  CAS  PubMed  Google Scholar 

  • Sapsford, K. E., Liron, Z., Shubin, Y. S. and Ligler, F. S. (2001). Kinetics of Antigen Binding to Arrays of Antibodies in Different Sized Spots. Anal Chem 73(22): 5518–24

    Article  CAS  PubMed  Google Scholar 

  • Sheng M. 2001. Molecular Organization of the Postsynaptic Specialization. Proc Natl Acad Sci U S A. 2001 Jun 19;98(13):7058–61

    Article  CAS  PubMed  Google Scholar 

  • Toyofuku T Yabuki M, Otsu K, Kuzuya T, Hori M, Tada M (1998). Direct Association of the Gap Junction Protein Connexin-43 with ZO-1 in Cardiac Myocytes. J Biol Chem 273(21): 12725–31

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2005 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Duffy, H.S., Iacobas, I., Spray, D.C., Ashton, A.W. (2005). Using Antibody Arrays to Detect Protein-Protein Interactions. In: Dhein, S., Mohr, F.W., Delmar, M. (eds) Practical Methods in Cardiovascular Research. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-26574-0_46

Download citation

  • DOI: https://doi.org/10.1007/3-540-26574-0_46

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-40763-8

  • Online ISBN: 978-3-540-26574-0

  • eBook Packages: MedicineMedicine (R0)

Publish with us

Policies and ethics