Abstract
Experimental assays based on living cells have emerged to an indispensable tool in the life sciences as a compromise between animal experiments and purely molecular interactions analysis. Label-free monitoring of such assays is rather new and its technical progress has been driven by the accumulating evidence that the molecular constituents of label-based approaches might manipulate the assay cells or their readout might be affected by the compound being tested in the assay. This has been particularly evident in the field of nanotoxicology as many nanomaterials are luminescent or redox active or they inhibit the activity of enzymes that are used to analyze the cell response. Among the established label-free techniques to monitor cell-based assays, impedance analysis is the farthest developed with respect to the available assay formats, throughput, and information content of the raw data. This chapter will summarize the general principles of impedimetric cell monitoring, introduce the available assay formats, and show how these have been applied to unravel the biological response of nanoscale particles on different levels of cell physiology. The description and interpretation of impedimetric assays will be embedded in a thorough discussion on the pros and cons of label-free versus label-based monitoring of animal cells in biomedical assays.
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Notes
- 1.
Impedance for the fraction of the total current that is in phase with the voltage
- 2.
Impedance for the fraction of the total current that is 90° out of phase with the voltage
- 3.
C = 1/[2·π·f·Im(Z)] with f as the AC frequency.
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Acknowledgments
The authors would like to thank the Deutsche Forschungsgemeinschaft (within SPP 1313, GRK 1910, GRK 1570) and the Federal Ministry for Economic Affairs and Energy (within ZIM projects FASTEST and THERANOSTIC) for substantial and continuous support. SL was supported by the European Union under Grant Agreement number 264772 (International Training Network CHEBANA).
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Sperber, M. et al. (2015). Monitoring the Impact of Nanomaterials on Animal Cells by Impedance Analysis: A Noninvasive, Label-Free, and Multimodal Approach. In: Wegener, J. (eds) Measuring Biological Impacts of Nanomaterials. Bioanalytical Reviews, vol 5. Springer, Cham. https://doi.org/10.1007/11663_2015_13
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