Abstract
The biocontrol of plant diseases by microorganisms is a promising alternative to the chemical pesticides. Serratia marcescens strain B2 effectively controls fungal diseases of cyclamen and rice. Biocontrol by S. marcescens strain B2 is mediated by the combined effects of plural chitinases, antibiotic prodigiosin, induced systemic resistance. Activity of S. marcescens is often negatively affected by abiotic and biotic factors and antibiotic biosynthesis of this bacterium is reduced under the influence of rice-associated bacteria. A genetically modified rice-indigenous bacterium was developed by introducing genes encoding for antifungal factors. Disease inhibitory genes were isolated from S. marcescens and put under the control of several types of promoters, which were isolated from the recipient. These genetically modified microorganisms effectively suppressed rice blast disease caused by Pyricularia oryzae and are not affected by abiotic or biotic factors. Introduction of disease inhibitory genes controlled by promoters and derived from the recipient is a useful technology for the development of biocontrol agents.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Adams, P. B., and Wong, J. A.-L., 1991, The effect of chemical pesticides on the infection of sclerotia of Sclerotinia minor by the biocontrol agent Sporidesmium sclerotivorum, Phytopathology, 81:1340–1343.
Akutsu, K., Hirata, A., Yamamoto, M., Hirayae, K., Okuyama S., and Hibi T., 1993, Growth inhibition of Botrytis spp. by Serratia marcescens B2 isolated from tomato phylloplane, Ann. Phytopathol. Soc. Jpn. 59:18–25.
Amarger, N., 2002, Genetically modified bacteria in agriculture, Biochimie 84:1061–1072.
Bauer, W. D., and Teplitski, M., 2001, Can plants manipulate bacterial quorum sensing?, Aust. J. Plant Physiol. 28:913–921.
Bonsall, R. F., Weller, D. M., Thomashow, L. S., 1997, Quantification of 2,4-diacetylphloroglucinol produced by fluorescent Pseudomonas spp. in vitro and in the rhizosphere of wheat, Appl. Environ. Microbiol. 63:951–955.
Bruton, B. D., Mitchell, F., Fletcher, J., Pair, S. D., Wayadande, A., Melcher, U., Brady, J., Bextine, B., and Popham, T. W., 2003, Serratia marcescens, a phloem-colonizing, squash bug-transmitted bacterium: causal agent of cucurbit yellow vine disease, Plant Dis. 87:937–944.
Burpee, L. L., 1990, The influence of abiotic factors on biological control of soilborne plant pathogenic fungi, Can. J. Plant Pathol. 12:308–317.
Campbell, R., 1989, Biological Control of Microbial Plant Pathogens, Cambridge University Press., Cambridge, UK.
Chernin, L. S., de la Fuente, L., Sobolev, V., Haran, S., Vorgias, C. E., Oppenheim, A. B., and Chet, I., 1997, Molecular cloning, structural analysis, and expression in Escherichia coli of a chitinase gene from Enterobacter agglomerans, Appl. Environ. Microbiol. 63:834–839.
Chernin, L. S., Winson, M. K., Thompson, J. M., Haran, S., Bycroft, B. W., Chet, I., Williams, P., and Stewart, G. S. A. B., 1998, Chitinolytic activity in Chromobacterium violaceum: Substrate analysis and regulation by quorum sensing, J. Bactreriol. 180:4435–4441.
Cook, R. J., 1996, Assuring the safe use of microbial biocontrol agents: a need for policy based on real rather than perceived risks, Can. J. Plant Pathol. 18:439–445.
Cook, R. J., Bruckart, W. L., Coulson, J. R., Goettel, M. S., Humber, R. A., Lumsden, R. D., Maddox, J. V., McManus, M. L., Moore, L., Meyer, S. F., Quimby Jr., P. C., Stack, J. P., and Vaughn, J. L., 1996, Safety of microorganisms intended for pest and plant disease control: a framework for scientific evaluation, Biol. Control 7:333–351.
De Boer, M., Bom, P., Kindt, F., Keurentjes, J. J. B., Van der Sluis, I., Van Loon, L. C., and Bakker, P. A. H. M., 2003, Control of Fusarium wilt of radish by combining Pseudomonas putida strains that have different disease-suppressive mechanisms, Phytopathology 93:626–632.
De Leij, F. A. A. M., Sutton, E. J., Whipps, J. M., Fenlon, J. S., and Lynch, J. M., 1995, Impact of field release of genetically modified Pseudomonas fluorescens on indigenous microbial populations of wheat, Appl. Environ. Microbiol. 61:3443–3453.
Delany, I. R., Walsh, U. F., Ross, I., Fenton, A. M., Corkery, D. M., and O’Gara, F., 2001, Enhancing the biocontrol efficacy of Pseudomonas fluorescens F113 by altering the regulation and production of 2,4-diacetylphloroglucinol, Plant Soil 232:195–205.
Desai, S., Reddy, M. S., and Kloepper, J. W., 2002, Comprehensive testing of biocontrol agents, in: Biological Control of Crop Diseases, S. S. Gnanamanickam ed., Marcell Dekker Inc., NY, pp. 387–420.
Dowling, D. N., and O’Gara, F., 1994, Metabolites of Pseudomonas involved in the biocontrol of plant disease, Trends Biotechn. 12:133–141.
Downing, K. J., and Thomson, J. A., 2000, Introduction of the Serratia marcescens chiA gene into an endophytic Pseudomonas fluorescens for the biocontrol of phytopathogenic fungi, Can. J. Microbiol. 46:363–369.
Duffy, B. K., and Défago, G., 1997, Zinc improves biocontrol of Fusarium crown and root rot of tomato by Pseudomonas fluorescens and represses the production of pathogen metabolites inhibitory to bacterial antibiotic biosynthesis, Phytopathology 87:1250–1257.
Duffy, B. K., and Défago, G., 1999, Environmental factors modulating antibiotic and siderophore biosynthesis by Pseudomonas fluorescens biocontrol strains, Appl. Environ. Microbiol. 65:2429–2438.
Duffy, B., Schouten, A., and Raaijmakers, J. M., 2003, Pathogen self-defence: mechanisms to counteract microbial antagonism, Annu. Rev. Phytopathol. 41:501–538.
Fray, R. G., Throup, J. P., Daykin, M., Wallace, A., Williams, P., Stewart, G. S. A. B., and Grierson, D., 1999, Plants genetically modified to produce N-acylhomoserine lactones communicate with bacteria, Nature Biotechn. 17:1017–1020.
Fuchs, R. L., McPherson, S. A., and Drahos, D. J., 1986, Cloning of a Serratia marcescens gene encoding chitinase, Appl. Environ. Microbiol. 51:504–509.
Fukui, R., 2003, Suppression of soilborne plant pathogens through community evolution of soil microorganisms, Microb. Environ. 18:1–9.
Fukui, R., Fukui, H., and Alvarez, A. M., 1999, Comparisons of single versus multiple bacterial species on biological control of anthurium blight, Phytopathology 89:366–373.
Giddings, G., 1998, Transley review no. 99. The release of genetically engineered microorganisms and viruses into the environment, New Phytol. 140:173–184.
Gooday, G. W., 1990, Physiology of microbial degradation of chitin and chitosan, Biodegradation 1:177–190.
Grimont, P. A. D., and Grimont, F., 1978, The genus Serratia, Ann. Rev. Microbiol. 32:221–248.
Gullino, M. L., Migheli, Q., and Mezzalama, M., 1995, Risk analysis in the release of biological control agents, Plant Dis. 79:1193–1201.
Guo, J.-H., Qi, H.-Y., Guo, Y.-H., Ge, H.-L., Gong, L.-Y., Zhang, L.-X., and Sun, P.-H., 2004, Biocontrol of tomato wilt by plant growth-promoting rhizobacteria, Biol. Control 29:66–72.
Haas, D., and Keel, C., 2003, Regulation of antibiotic production in root-colonizing Pseudomonas spp. and relevance for biological control of plant disease, Annu. Rev. Phytopathol. 41:117–153.
Hejazi, A., and Falkiner, F. R., 1997, Serratia marcescens, J. Med. Microbiol. 46:903–912.
Herrera-Estrella, A., and Chet, I., 1999, Chitinases in biological control, in: Chitin and Chitinases, P. Jollès, and R. A. A. Muzzarelli, eds., Birkhäuser Verlag, Basel, pp. 171–184.
Hirayae, K., Hirata, A., Akutsu, K., Hara, S., Havukkala, I., Nishizawa, Y., and Hibi T., 1996, In vitro growth inhibition of plant pathogenic fungi, Botrytis spp., by Escherichia coli transformed with a chitinolytic enzyme gene from a marine bacterium, Alteromonas sp. strain 79401, Ann. Phytopathol. Soc. Jpn. 62:30–36.
Horng, Y.-T., Deng, S.-C., Daykin, M., Soo, P.-C., Wei, J.-R., Luh, K.-T., Ho, S.-W., Swift, S., Lai, H.-C., and Williams, P., 2002, The LuxR family protein SpnR functions as a negative regulator of N-acylhomoserine lactone-dependent quorum sensing in Serratia marcescens, Mol. Microbiol. 45:1655–1671.
Ikeda, S., Toyoda, H., Matsuda, Y., Kurokawa, M., Tamai, T., Yoshida, K., Nami, C., Ikemoto, T., Enomoto, M., Shiraishi, K., Miyamoto, S., Hanaoka, M., and Ouchi, S., 1996, Cloning of a chitinase gene chiSH1 cloned from gram-positive bacterium Kurthia zopfii and control of powdery mildew of barley, Ann. Phytopathol. Soc. Jpn. 62:11–16.
Iyozumi, H., Komagata, T., Hirayae, K., Tsuchiya, K., Hibi, T., and Akutsu, K., 1996, Biological control of cyclamen gray mould (Botrytis cinerea) by Serratia marcescens B2, Ann. Phytopathol. Soc. Jpn. 62:559–565.
Kobayashi, D. Y., Guglielmoni, M., and Clarke, B. B., 1995, Isolation of the chitinolytic bacteria Xanthomonas maltophilia and Serratia marcescens as biological control agents for summer patch disease of turfgrass, Soil Biol. Biochem. 27:1479–1487.
Kraus, J., and Loper, J. E., 1995, Characterization of a genomic region required for production of the antibiotic pyoluteorin by the biological control agent Pseudomonas fluorescens Pf-5, Appl. Environ. Microbiol. 61:849–854.
Kravchenko, L. V., Azarova, T. S., Leonova-Erko, E. I., Shaposhnikov, A. I., Makarova, N. M., and Tikhonovich, I. A., 2003, Root exudates of tomato plants and their effect on the growth and antifungal activity of Pseudomonas strains, Microbiology 72:37–41.
Kredics, L., Antal, Z., Manczinger, L., Szekeres, A., Kevei, F., and Nagy, E., 2003., Influence of environmental parameters on Trichoderma strains with biocontrol potential, Food Technol. Biotech. 41:37–42.
Landa, B. B., Navas-Cortés, J. A., Hervás, A., and Jiménez-Diaz, R. M., 2001, Influence of temperature and inoculum density of Fusarium oxysporum f. sp. ciceris on suppression of Fusarium wilt of chickpea by rhizosphere bacteria, Phytopathology 91:807–816.
Landa, B. B., Cachinero-Diaz, J. M., Lemanceau, P., Jiménez-Diaz, R. M., and Alabouvette, C., 2002, Effect of fusaric acid and phytoanticipins on growth of rhizobacteria and Fusarium oxysporum, Can. J. Microbiol. 48:971–985.
Liu, L., Kloepper, J. W., and Tuzun, S., 1995, Induction of systemic resistance in cucumber against Fusarium wilt by plant growth-promoting rhizobacteria, Phytopathology 85:695–698.
Lutz, M. P., Feichtinger, G., Défago, G., and Duffy, B., 2003, Mycotoxigenic Fusarium and deoxynivalenol production repress chitinase gene expression in the biocontrol agent Trichoderma atroviride P1, Appl. Environ. Microbiol. 69:3077–3084.
Mathre, D. E., Cook, R. J., and Callan, N. W., 1999, From discovery to use-traversing the world of commercializing biocontrol agents for plant disease control, Plant Dis. 83:972–983.
Maurhofer, M., Keel, C., Schnider, U., Voisard, C., Haas, D., and Défago, G., 1992, Influence of enhanced antibiotic production in Pseudomonas fluorescens strain CHA0 on its disease suppressive capacity, Phytopathology 82:190–195.
Mukohara, Y., 1998, Aspect for development of biological control agents (in Japanese), BIO INDUSTRY 15:31–40.
Natsch, A., Keel, C., Hebecker, N., Laasik, E., and Défago, G., 1997, Influence of biocontrol strain Pseudomonas fluorescens CHA0 and its antibiotic overproducing derivative on the diversity of resident root colonizing pseudomonads, FEMS Microbiol. Ecol. 23:341–352.
Notz, R., Maurhofer, M., Schnider-Keel, U., Duffy, B., Haas, D., and Défago, G., 2001, Biotic factors affecting expression of the 2,4-diacetylphloroglucinol biosynthesis gene phlA in Pseudomonas fluorescens biocontrol strain CHA0 in the rhizosphere, Phytopathology 91:873–881.
Notz, R., Maurhofer, M., Dubach, H., Haas, D., and Défago, G., 2002, Fusaric acid-producing strains of Fusarium oxysporum alter 2,4-diacetylphloroglucinol biosynthetic gene expression in Pseudomonas fluorescens CHA0 in vitro and in the rhizosphere of wheat, Appl. Environ. Microbiol. 68:2229–2235.
Numata, S., Ui, S., Tomiyama, M., Hasebe, A., Nakajima, M., and Akutsu, K., 2004, Cloning of various promoters for foreign gene expression in Erwinia ananas, J. Gen. Plant Pathol. 70:69–73.
Okamoto, H., Sato, M., Sato, Z., and Isaka, M., 1998, Biocontrol of Phytophthora capsici by Serratia marcescens F-1-1 and analysis of biocontrol mechanisms using transposon-insertion mutants, Ann. Phytopathol. Soc. Jpn. 64:287–293.
Ordentlich, A., Elad, Y., and Chet, I., 1988, The role of chitinase of Serratia marcescens in biocontrol of Sclerotium rolfsii, Phytopathology 78:84–88.
Ownley, B. H., Weller, D. M., and Thomashow, L. S., 1992, Influence of in situ and in vitro pH on suppression of Gaeumannomyces graminis var. tritici by Pseudomonas fluorescens 2–79, Phytopathology 82:178–184.
Ownley, B. H., Duffy, B. K., and Weller, D. M., 2003, Identification and manipulation of soil properties to improve the biological control performance of phenazine-producing Pseudomonas fluorescens, Appl. Environ. Microbiol. 69:3333–3343.
Pierson, E. A., and Weller, D. M., 1994, Use of mixtures of fluorescent pseudomonads to suppress take-all and improve the growth of wheat, Phytopathology 84:940–947.
Pierson III, L. S., and Pierson, E. A., 1996, Phenazine antibiotic production in Pseudomonas aureofaciens: role in rhizosphere ecology and pathogen suppression, FEMS Microbiol. Lett. 136:101–108.
Raaijmakers, J. M., Weller, D. M., and Thomashow, L. S., 1997, Frequency of antibiotic-producing Pseudomonas spp. in natural environments, Appl. Environ. Microbiol. 63:881–887.
Rascoe, J., Berg, M., Melcher, U., Mitchell, F. L., Bruton, B. D., Pair, S.D., and Fletcher, J., 2003, Identification, phylogenic analysis, and biological characterization of Serratia marcescens strains causing cucurbit yellow vine disease, Phytopathology 93:1233–1239.
Raupach, G. S., Liu, L., Murphy, J. F., Tuzun, S., and Kloepper, J. W., 1996, Induced systemic resistance in cucumber and tomato against cucumber mosaic cucumovirus using plant growth-promoting rhizobacteria (PGPR), Plant Dis. 80:891–894.
Ryder, M., 1994, Key issues in the deliberate release of genetically-manipulated bacteria, FEMS Microbiol. Ecol. 15:139–146.
Schisler, D. A., Slininger, P. J., and Bothast, R. J., 1997, Effects of antagonist cell concentration and two-strain mixtures on biological control of Fusarium dry rot of potatoes, Phytopathology 87:177–183.
Schmidt, C. S., Agostini, F., Leifert, C., Killham, K., and Mullins, C. E., 2004, Influence of soil temperature and matric potential on sugar beet seedling colonization and suppression of Pythium damping-off by the antagonistic bacteria Pseudomonas fluorescens and Bacillus subtilis, Phytopathology 94:351–363.
Schnider-Keel, U., Seematter, A., Maurhofer, M., Blumer, C., Duffy, B., Gigot-Bonnefoy, C., Reimmann, C., Notz, R., Défago, G., Haas, D., and Keel, C., 2000, Autoinduction of 2,4-diacetylphloroglucinol biosynthesis in the biocontrol agent Pseudomonas fluorescens CHA0 and repression by the bacterial metabolites salicylate and pyoluteorin, J. Bacteriol. 182:1215–1225.
Schroth, M. N., and Hancock, J. G., 1982, Disease-suppressive soil and root-colonizing bacteria, Science 216:1376–1381.
Shanahan, P., O’sullivan, D. J., Simpson, P., Glennon, J. D., O’Gara, F., 1992, Isolation of 2,4-diacetylphloroglucinol from a fluorescent pseudomonad and investigation of physiological parameters influencing its production, Appl. Environ. Microbiol. 58:353–358.
Shapira, R., Ordentlich, A., Chet, I., and Oppenheim, A. B., 1989, Control of plant diseases by chitinase expressed from cloned DNA in Escherichia coli, Phytopathology 79:1246–1249.
Shipton, P. J., Cook, R. J., and Sitton, J. W., 1973, Occurrence and transfer of a biological factor in soil that suppresses take-all of wheat in eastern Washington, Phytopathology 63:511–517.
Someya, N., Kataoka, N., Komagata, T., Hirayae, K., Hibi, T., and Akutsu, K., 2000, Biological control of cyclamen soilborne diseases by Serratia marcescens strain B2, Plant Dis. 84:334–340.
Someya, N., Nakajima, M., Hirayae, K., Hibi, T., and Akutsu, K., 2001, Synergistic antifungal activity of chitinolytic enzymes and prodigiosin produced by biocontrol bacterium, Serratia marcescens strain B2 against gray mold pathogen, Botrytis cinerea, J. Gen. Plant Pathol. 67:312–317.
Someya, N., Nakajima, M., Hibi, T., Yamaguchi, I., and Akutsu, K., 2002, Induced resistance to rice blast by antagonistic bacterium, Serratia marcescens strain B2, J. Gen. Plant Pathol. 68:177–182.
Someya, N., Nakajima, M., Watanabe, K., Hibi, T., and Akutsu, K., 2003a, Influence of bacteria isolated from rice plants and rhizospheres on antibiotic production by the antagonistic bacterium Serratia marcescens strain B2, J. Gen. Plant Pathol. 69:342–347.
Someya, N., Numata, S., Nakajima, M., Hasebe, A., Hibi, T., and Akutsu, K., 2003b, Biological control of rice blast by the epiphytic bacterium Erwinia ananas transformed with a chitinolytic enzyme gene from an antagonistic bacterium, Serratia marcescens strain B2, J. Gen. Plant Pathol. 69:276–282.
Someya, N., Nakajima, M., Hamamoto, H., Yamaguchi, I., and Akutsu, K., 2004, Effects of light conditions on prodigiosin stability in the biocontrol bacterium Serratia marcescens strain B2, J. Gen. Plant Pathol. 70:in press.
Someya, N., Nakajima, M., Watanabe, K., Hibi, T., and Akutsu, K., 2005a, Potential of Serratia marcescens strain B2 for biological control of rice sheath blight, Biocontrol Sci. Techn. 15:in press.
Someya, N., Numata, S., Nakajima, M., Hasebe, A., Akutsu, K., 2005b, Influence of riceisolated bacteria on chitinase production by the biocontrol bacterium Serratia marcescens strain B2 and the genetically modified rice epiphytic bacterium, J. Gen. Plant Pathol. 71:in press.
Stephenson, J. R., and Warnes, A., 1996, Release of genetically modified micro-organisms into the environment, J. Chem. Tech. Biotechnol. 65:5–14.
Stewart, A., 2001, Commercial biocontrol-reality or fantasy?, Austral. Plant Pathol. 30:127–131.
Sundheim, L., Poplawsky, A. R., and Ellingboe, A. H., 1988, Molecular cloning of two chitinase genes from Serratia marcescens and their expression in Pseudomonas species, Physiol. Mol. Plant Pathol. 33:483–491.
Swift, S., Throup, J. P., Williams, P., Salmond, G. P. C., and Stewart, G. S. A. B., 1996, Quorum sensing: a population-density component in the determination of bacterial phenotype, Trends Biochem. Sci. 21:214–219.
Teplitski, M., Robinson, J. B., and Bauer, W. D., 2000, Plants secrete substances that mimic bacterial N-acyl homoserine lactone signal activities and affect population densitydependent behaviors in associated bacteria, Mol. Plant-Microbe Interact. 13:637–648.
Thomson, N. R., Crow, M. A., McGowan, S. J., Cox, A., and Salmond, G. P. C., 2000, Biosynthesis of carbapenem antibiotic and prodigiosin pigment in Serratia is under quorum sensing control, Mol. Microbiol. 36:539–556.
Toyota, K., Miyashita, K., and Kimura, M., 1994, Introduction of a chitinase gene into Pseudomonas stutzeri A18 isolated from the surface of chlamydospores of Fusarium oxysporum f. sp. raphani, Soil Biol. Biochem. 26:413–416.
Utkhede, R. S., 1996, Potential and problems of developing bacterial biocontrol agents, Can. J. Plant Pathol. 18:455–462.
Van Rij, E. T., Wesselink, M., Chin-A-Woeng, T. F. C., Bloemberg, G. V., and Lugtenberg, B. J. J., 2004, Influence of environmental conditions on the production of phenazine-1-carboxamide by Pseudomonas chlororaphis PCL1391, Mol. Plant-Microbe Interact. 17:557–566.
Vidhyasekaran, P., 2004, Biological control-microbial pesticides, in: Concise Encyclopedia of Plant Pathology, Food Products Press and the Haworth Reference Press, NY, pp. 239–270.
Walker, T. S., Bais, H. P., Grotewold, E., and Vivanco, J. M., 2003, Root exudation and rhizosphere biology, Plant Physiol. 132:44–51.
Walsh, U. F., Morrissey, J. P., and O’Gara, F., 2001, Pseudomonas for biocontrol of phytopathogens: from functional genomics to commercial exploitation, Curr. Opin. Biotechnol. 12:289–295.
Wei, G., Kloepper, J. W., and Tuzun, S., 1996, Induced systemic resistance to cucumber diseases and increased plant growth by plant growth-promoting rhizobacteria under field conditions, Phytopathology 86:221–224.
Wilson, M., and Lindow, S. E., 1993, Release of recombinant microorganisms, Annu. Rev. Microbiol. 47:913–944.
Wood, D. W., Gong, F., Daykin, M. M., Williams, P., and Pierson III, L. S., 1997, N-acylhomoserine lactone-mediated regulation of phenazine gene expression by Pseudomonas aureofaciens 30–84 in the wheat rhizosphere, J. Bacteriol. 179:7663–7670.
Yoda, J., 2004, Establishment of the bill on living modified organisms (LMOs) and its background (in Japanese), Protein, Nucleic acid and Enzyme 49:559–566.
Zhou, H., Yao, F., Roberts, D. P., and Lessie, T. G., 2003, AHL-deficient mutants of Burkholderia ambifaria BC-F have decreased antifungal activity, Curr. Microbiol. 47:174–179.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2005 Springer
About this chapter
Cite this chapter
Someya, N., Akutsu, K. (2005). Biocontrol of Plant Diseases by Genetically Modified Microorganisms: Current Status and Future Prospects. In: Siddiqui, Z.A. (eds) PGPR: Biocontrol and Biofertilization. Springer, Dordrecht. https://doi.org/10.1007/1-4020-4152-7_11
Download citation
DOI: https://doi.org/10.1007/1-4020-4152-7_11
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-4002-3
Online ISBN: 978-1-4020-4152-5
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)