Skip to main content
SpringerLink
Log in

Glucose Sensing and Glucose Determination Using Fluorescent Probes and Molecular Receptors

  • Chapter
Glucose Sensing

Part of the book series: Topics in Fluorescence Spectroscopy ((TIFS,volume 11))

Abstract

We summarize our previous and recent work on the use of fluorescent probes and of synthetic molecular receptors (including polymeric receptors and bead-immobilized receptors) for determination of glucose. The following approaches were made: (1) for enzyme-based quantitation of glucose in solution, the fluorescent probes were added to the sample solution along with glucose oxidase (GOx) and kinetics was followed over time. Specifically, the metal ligand complex Ru(sulfo-dpp)3 served as a water-soluble probe for oxygen, while the europium-tetracycline complex served as a probe for hydrogen peroxide; (2) for enzyme-based (continuous) glucose sensing, GOx is immobilized (along with indicator probes) on certain hydrogels which results in sensor layers that give an optical response to glucose via the indicator dye employed; (3) in an entirely different approach, a ruthenium-ligand complex with a boronic acid side group was synthesized and studied for its response to polyols and certain monosaccharides; (4) in yet another approach, a polymeric saccharide-responsive material was obtained by copolymerization of aniline with aniline boronic acid to give a copolymer with a near-infrared (600 – 900 nm) optical response (in absorption); (5) the enzyme-based schemes subsequently were applied to lifetime-based imaging of the distribution of glucose and GOx.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Similar content being viewed by others

References

  1. B. Valeur, Molecular Fluorescence: Principles and Applications, (Wiley — VCH, Weinheim, 2002).

    Google Scholar 

  2. (a) J. S. Schultz, S. Mansouri and I. J. Goldstein, Diabetes Care 5, 245 (1982); (b) R. Ballerstadt and J. S. Schultz, Anal. Chem. 72, 4185 (2000).

    Article  Google Scholar 

  3. G. Liebsch, I. Klimant, Ch. Krause and O. S. Wolfbeis, Anal. Chem. 73, 4354 (2001).

    Article  Google Scholar 

  4. R. Narayanaswamy, and F. Sevilla, Anal. Lett. 21, 1165 (1988).

    Google Scholar 

  5. M. M. F. Choi, Microchim. Acta 148, 107–132 (2004).

    Article  Google Scholar 

  6. A. Kumagai, H. Suzuki, K. Ogawa and E. Kokufuta, Chem. Sensors 19, 1 (2003).

    Google Scholar 

  7. C. C. Liu and M. R. Neuman, Diabetes Care 5, 275 (1982).

    Article  Google Scholar 

  8. I. Rocha and E. C. Ferreira, Anal. Chim. Acta 462, 293 (2002).

    Article  Google Scholar 

  9. M. G. Sasso, F. H. Quina, and E. J. H. Bechara, Anal. Biochem. 156, 239 (1986).

    Article  Google Scholar 

  10. J. M. Vanderkooi, G. Maniara, T. J. Green and D. F. Wilson, J. Biol. Chem. 262, 5476 (1987).

    Google Scholar 

  11. W. Treiber, J. Siedel, and B. Vogt, Ger. Offen. DE 4309394 (1994).

    Google Scholar 

  12. M. V. Cattaneo and J. H. Luong, Anal. Biochem. 223, 313 (1994).

    Article  Google Scholar 

  13. F. Wang, Y. Wu, X. Wu, S. Sun, Y. Ci, Fresenius’ J. Anal. Chem. 346, 1011 (1993).

    Article  Google Scholar 

  14. M. Mifune, K. Sugimoto, A. Iwado, H. Akizawa, N. Motohashi and Y. Saito, Anal. Sci. 19, 569 (2003).

    Article  Google Scholar 

  15. Y. Nakajima and Y. Goto, Jpn. Kokai Tokkyo Koho JP 121637 (2000).

    Google Scholar 

  16. D. J. Capaldi and K. E. Taylor, Anal. Biochem. 129, 329 (1983).

    Article  Google Scholar 

  17. Z.-X. Guo, H.-X. Shen and L. Li, Microchim. Acta 131, 171 (1999).

    Article  Google Scholar 

  18. W. Werner, H. G. Rey and H. Wielinger, Fresenius’ J. Anal. Chem. 252, 224 (1970).

    Article  Google Scholar 

  19. D. Barham, P. Trinder, Analyst 97, 142 (1972).

    Article  ADS  Google Scholar 

  20. S. Charlton and E. Kurchacova, US Pat. 4,855,228 (1989).

    Google Scholar 

  21. M. R. Slaughter, P. J. O’Brien, Clin. Biochem. 33, 525 (2000).

    Article  Google Scholar 

  22. Z.-X. Guo, H.-X. Shen and L. Li, Microchem. J., 61, 134 (1999).

    Article  Google Scholar 

  23. F. A. El-Essi, A. Z. A. Zuhri, S. I. Al-Khalil and M. S. Abdel-Latif, Talanta 44, 2051 (1997).

    Article  Google Scholar 

  24. G. G. Guilbault, S. S. Kuan and P. J. Brignac Jr., Anal. Chim. Acta 47, 503 (1969).

    Article  Google Scholar 

  25. J. Meyer and U. Karst, Analyst 125, 1537 (2000).

    Article  ADS  Google Scholar 

  26. G. G. Guilbault, D. N. Kramer and E. B. Hackley, Anal. Chem. 39, 271 (1967).

    Article  Google Scholar 

  27. G. G. Guilbault, P. Brignac and M. Zimmer, Anal Chem. 40, 190 (1968).

    Article  Google Scholar 

  28. G. G. Guilbault, P. Brignac and M. Juneau, Anal Chem. 40, 1256 (1968).

    Article  Google Scholar 

  29. H. Maeda, S. Matsu-Ura, M. Nishida, T. Senba, Y. Yamauchi and H. Ohmori. Chem. Pharm. Bull. 49, 294 (2001).

    Article  Google Scholar 

  30. S. Matsu-Ura, Y. Yamauchi, H. Ohmori and H. Maeda, Bunseki Kagaku 51, 111 (2002).

    Article  Google Scholar 

  31. M. Zhou, Z. Diwu, N. Panchuk-Voloshina and R. P. Haugland, Anal. Biochem. 253, 162 (1997).

    Article  Google Scholar 

  32. R. Haugland, Handbook of Fluorescent Probes and Research Products (9th ed.; Eugene, OR, 2002) pp.414, 443; htt://www.probes.com/media/pis/mp22189.pdf.

    Google Scholar 

  33. M. Zhou, C. Zhang, R. H. Upson and R. P. Haugland, Anal. Biochem. 260, 257 (1998).

    Article  Google Scholar 

  34. M. Wu, Z. Lin, A. Duerkop and O. S. Wolfbeis, Anal. Bioanal. Chem. 380, 619 (2004).

    Article  Google Scholar 

  35. O. S. Wolfbeis, A. Duerkop, M. Wu, Zh. Lin, Angew. Chem. Intl. Ed. 41 4495 (2002).

    Article  Google Scholar 

  36. O. S. Wolfbeis, Fiber Optic Chemical Sensors and Biosensors (CRC Press, Boca Raton, 1991, vol. 2).

    Google Scholar 

  37. B. H. Weigl and O. S. Wolfbeis, Anal Chem. 66, 3323 (1994).

    Article  Google Scholar 

  38. O. S. Wolfbeis, M. Schaeferling and A. Duerkop, Microchim. Acta 143 221 (2003).

    Article  Google Scholar 

  39. Ch. Huber, T. Werner, Ch. Krause and O. S. Wolfbeis, Microchim. Acta 142 245 (2003).

    Article  Google Scholar 

  40. W. Trettnak, M. J. P. Leiner and O. S. Wolfbeis, Biosensors 4, 15 (1988).

    Article  Google Scholar 

  41. M. Gouterman, J. Callis, L. Dalton, G. Khalil, Y. Mebarki, K. R Cooper and M. Grenier, Meas. Sci. Technol. 5, 1986 (2004).

    Article  ADS  Google Scholar 

  42. B. M. Weidgans, C. Krause, I. Klimant and O. S. Wolfbeis, Analyst 129, 645 (2004).

    Article  ADS  Google Scholar 

  43. H. R Kermis, Y. Kostov, P. Harms and G. Rao, Biotechnol. Progress 18, 1047 (2002).

    Article  Google Scholar 

  44. S.-H. Lee, K. G. Chittibabu, J. Kim, J. Kumar, and S. K. Tripathy, Polym. Mat. Sci. Eng. 83, 250 (2000).

    Google Scholar 

  45. G. J. Mohr and O. S. Wolfbeis, Anal. Chim. Acta 292 41 (1994).

    Article  Google Scholar 

  46. U. Kosch, I. Klimant, T. Werner, O. S. Wolfbeis, Anal. Chem. 70 3892–3897 (1998) and refs. cited

    Article  Google Scholar 

  47. J. I. Peterson, S. R Goldstein, R. V. Fitzgerald and D. K. Buckhold, Anal. Chem. 52, 864 (1980).

    Article  Google Scholar 

  48. D. B. Papkovsky, G. V. Ponomarev, O. S. Wolfbeis, J. Photochem. Photobiol. A 104151 (1997).

    Google Scholar 

  49. S. A. Piletsky, T. L. Panasyuk, E. V. Piletskaya, T. A. Sergeeva, A. V. Elskaya, E. Pringsheim and O. S. Wolfbeis, Fresenius’ J. Anal. Chem. 366, 807 (2000).

    Article  Google Scholar 

  50. D. W. Luebbers and N. Opitz, Sens. Actuators 4, 641 (1983).

    Article  Google Scholar 

  51. W. Trettnak, M. J. P. Leiner and O. S. Wolfbeis, Analyst 113, 1519 (1988).

    Article  ADS  Google Scholar 

  52. B. P. H. Schaffar and O. S. Wolfbeis, Biosensors Bioelectron. 5, 137–148 (1990).

    Article  Google Scholar 

  53. O. S. Wolfbeis, M. J. P. Leiner and H. E. Posch, Microchim. Acta III, 359 (1986).

    Article  Google Scholar 

  54. M. C. Moreno-Bondi, O. S. Wolfbeis, M. J. P. Leiner and B. P. H. Schaffar, Anal. Chem. 62, 2377 (1990).

    Article  Google Scholar 

  55. I. Klimant, F. Ruckruh, G. Liebsch, A. Stangelmayer and O. S. Wolfbeis, Mikrochim. Acta 131, 35 (1999).

    Article  Google Scholar 

  56. O. S. Wolfbeis, I. Oehme, N. Papkovskaya and I. Klimant, Biosensors Bioelectron. 15, 69 (2000).

    Article  Google Scholar 

  57. Z. Rosenzweig, R. Kopelman, Anal. Chem. 68, 1408 (1996).

    Article  Google Scholar 

  58. C. McDonagh, B. D. MacCraith and A. K. McEvoy, Anal. Chem. 70, 45 (1998)

    Article  Google Scholar 

  59. A. Apostolidis, I. Klimant, D. Andrzejewski and O. Wolfbeis, J. Combinat. Chem. 6, 325 (2004).

    Article  Google Scholar 

  60. S. Lee and I. Okura, Anal. Commun. 34, 185 (1997).

    Article  Google Scholar 

  61. Y. Amao, T. Miyashita and I. Okura, J. Porphyr. Phthalocyan. 5(5), 433 (2001).

    Article  Google Scholar 

  62. D. V. Papkovsky, G.V. Ponomarev, W. Trettnak and P. O’Leary, Anal. Chem. 67, 4112 (1995).

    Article  Google Scholar 

  63. F. Alava-Moreno, M. Valencia-Gonzalez, A. Sanz-Medel and M. Diaz-Garcia, Analyst 122, 807 (1997).

    Article  ADS  Google Scholar 

  64. B. P. H. Schaffar and O. S. Wolfbeis, Proc. SPIE 990, 122 (1989).

    Google Scholar 

  65. H. E. Posch and O. S. Wolfbeis, Microchim. Acta I, 41 (1989).

    Article  Google Scholar 

  66. V. A. Stoy, J. Biomaterials Appl. 3, 552, (1989)

    Article  Google Scholar 

  67. G. Deng, T. D. James and S. Shinkai, J. Am. Chem. Soc. 116, 4567 (1994).

    Article  Google Scholar 

  68. J. Yoon, A. W. Czarnik, J. Am. Chem. Soc., 114, 5874 (1992).

    Article  Google Scholar 

  69. O. S. Wolfbeis, I. Klimant, T. Werner, Ch. Huber, U. Kosch, Ch. Krause, G. Neurauter and A. Duerkop, Sensors Actuat. B 51, 17 (1998).

    Article  Google Scholar 

  70. Z. Ge, C. W. Brown, L. Sun and S. C. Yang, Anal. Chem. 65, 2335 (1993).

    Article  Google Scholar 

  71. E. Pringsheim, E. Terpetschnig and O. S. Wolfbeis, Anal. Chim. Acta 357, 247 (1997).

    Article  Google Scholar 

  72. R. Koncki and O. S. Wolfbeis, Biosensors Bioelectron. 14, 87 (1999).

    Article  Google Scholar 

  73. E. Pringsheim, E. Terpetschnig, S. A. Piletsky and O. S. Wolfbeis, Adv. Mater. 11, 865 (1999).

    Article  Google Scholar 

  74. B. Meier, T. Werner, I. Klimant and O. S. Wolfbeis, Sens. Actuators B 29, 240 (1995).

    Article  Google Scholar 

  75. O. S. Wolfbeis, The Fluorescence of Organic Natural Products, in: Molecular Luminescence Spectroscopy: Methods & Applications, Vol. 1, edited by S. G. Schulman (Wiley, New York, 1985), pp. 167–369.

    Google Scholar 

  76. W. Trettnak and O. S. Wolfbeis, Anal. Chim. Acta 221, 195 (1989).

    Article  Google Scholar 

  77. Sanz, V; Galban, J; de Marcos, S. and Castillo, J. R., Talanta 60, 415 (2003).

    Article  Google Scholar 

  78. Sanz-Vicente, I.; Castillo, J. R. and Galban, J., Talanta 65, 946 (2005).

    Article  Google Scholar 

  79. P. Babilas, V. Schacht, G. Liebsch, O. S. Wolfbeis, M. Landthaler, R. M. Szeimies and C. Abels, Brit. J. Cancer 88, 1462 (2003).

    Article  Google Scholar 

  80. P. Babilas, G. Liebsch, V. Schacht, I. Klimant, O. S. Wolfbeis, R. M. Szeimies and C. Abels, Microcirculation, in press (2005).

    Google Scholar 

  81. M. Schaeferling, Luminescence lifetime-based imaging of sensor arrays for high-throughput screening applications, in: Springer Series on Chemical Sensors and Biosensors, Vol. 3, edited by G. Orellana, M.C. Moreno-Bondi (Springer, Heidelberg, 2005).

    Google Scholar 

  82. R.J. Woods, S. Scypinski, L.J. Cline Love and H.A. Ashworth, Anal. Chem. 56, 1395 (1984).

    Article  Google Scholar 

  83. T. Q. Ni and L. A. Melton, Appl. Spectroscopy, 47, 773 (1993).

    Article  ADS  Google Scholar 

  84. P. Hartmann and W. Ziegler, Anal. Chem. 68, 4512 (1996).

    Article  Google Scholar 

  85. M. Schaeferling, M. Wu and O.S. Wolfbeis, J. Fluorescence 14, 561 (2004).

    Article  Google Scholar 

  86. M. Schaeferling, M. Wu, J. Enderlein, H. Bauer and O.S. Wolfbeis, Appl. Spectroscopy 57, 1386 (2003).

    Article  ADS  Google Scholar 

  87. J. R. Lakowicz, Principles of Fluorescence Spectroscopy, 2nd edn, (Kluwer Academic/Plenum Publishers, New York 1999).

    Google Scholar 

  88. E.R. Carraway, J.N. Demas, B.A. DeGraff and J.R. Bacon, Anal. Chem. 63, 337 (1991).

    Article  Google Scholar 

  89. D.B. Papkovsky, J. Olah, I.V. Troyanowsky, N.A. Sadowsky, V.D. Rumyantseva, A.F. Mironov, I. Yaropolov and A.P. Savitsky, Biosens. Bioelectron. 7, 199 (1991).

    Article  Google Scholar 

  90. P.J. Spellane, M. Gouterman, A. Antipas, S. Kim and Y.C. Liu, Inorg. Chem. 19, 386 (1980).

    Article  Google Scholar 

  91. W. Zhong, P. Urayama and M.-A. Mycek, J Phys D: Appl. Phys. 36, 1689 (2003).

    Article  ADS  Google Scholar 

  92. Y. Amao, Microchim. Acta 143, 1 (2003).

    Article  Google Scholar 

  93. G. Liebsch, I. Klimant I, B. Frank, G. Holst and O.S. Wolfbeis, Appl. Spectroscopy 54, 548 (2000).

    Article  ADS  Google Scholar 

  94. M. Wu, Z. Lin, M. Schaeferling, A. Duerkop and O. S. Wolfbeis, Anal. Biochem., 340, 66 (2005).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Analytical Chemistry, Chemo-and Biosensors, University of Regensburg, D-93040, Regensburg, Germany

    Axel Duerkop, Michael Schaeferling & Otto S. Wolfbeis

Authors
  1. Axel Duerkop
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Michael Schaeferling
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Otto S. Wolfbeis
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. The Institute of Fluorescence Medical Biotechnology Center, University of Maryland Biotechnology Institute, Baltimore, Maryland

    Chris D. Geddes

  2. Center for Fluorescence Spectroscopy and Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland

    Joseph R. Lakowicz

Rights and permissions

Reprints and permissions

Copyright information

© 2006 Springer Science+Business Media, Inc.

About this chapter

Cite this chapter

Duerkop, A., Schaeferling, M., Wolfbeis, O.S. (2006). Glucose Sensing and Glucose Determination Using Fluorescent Probes and Molecular Receptors. In: Geddes, C.D., Lakowicz, J.R. (eds) Glucose Sensing. Topics in Fluorescence Spectroscopy, vol 11. Springer, Boston, MA. https://doi.org/10.1007/0-387-33015-1_15

Download citation

Publish with us

Policies and ethics

Search

Navigation