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Signal Amplification Techniques: bDNA, Hybrid Capture

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Book cover Advanced Techniques in Diagnostic Microbiology

Abstract

Several molecular technologies are designed to avoid target amplification so to minimize the possibility of contamination by target amplification products. One of the alternatives to enzymatic amplification of target nucleic acid such as polymerase chain reaction (PCR) is to increase or amplify the signal generated from the probe molecule hybridized to the target nucleic acid sequence, which is referred to as signal amplification. Commonly used signal amplification technologies include branched DNA (bDNA) and hybrid capture (HC) assays. The bDNA method was initially developed by Chiron (Emeryville, CA, USA) and marketed by Bayer Diagnostics (Emeryville, CA, USA), and the hybrid capture method was developed and marketed by Digene Corporation (Gaithersburg, MD, USA).

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  1. Yun F. (Wayne) Wang
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Wang, Y.F. (2006). Signal Amplification Techniques: bDNA, Hybrid Capture. In: Advanced Techniques in Diagnostic Microbiology. Springer, Boston, MA. https://doi.org/10.1007/0-387-32892-0_14

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