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Reviews in Fluorescence 2005 pp 245–270Cite as

Time-Resolved Evanescent Wave-Induced Fluorescence Anisotropy Measurements

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Part of the book series: Reviews in Fluorescence ((RFLU,volume 2005))

11.1. Abstract

The technique of time-resolved evanescent wave-induced fluorescence spectroscopy (TR-EWIFS) has been extended to incorporate fluorescence anisotropy measurements. We report on the application of these time-resolved evanescent wave-induced fluorescence anisotropy measurements (EW-TRAMs) as a source of information concerning the motion and conformational changes of macromolecules that can occur near a solid/liquid interface. We have applied EW-TRAMs to studies of the adsorption of proteins and polymers onto silica from solution. We also discuss the implications and potential complications of the polarisation properties of the standing evanescent field and how these properties can be used to advantage

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Authors and Affiliations

  1. School of Chemistry, University of Melbourne, Parkville, 3010, Victoria, Australia

    Trevor A. Smith, Michelle L. Gee & Colin A. Scholes (PhD student)

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  1. Trevor A. Smith
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  1. Institute of Fluorescence and Center for Fluorescence Spectroscopy, University of Maryland Biotechnology Institute, Baltimore, Maryland

    Chris D. Geddes

  2. Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Baltimore, Maryland

    Joseph R. Lakowicz

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Smith, T.A., Gee, M.L., Scholes, C.A. (2005). Time-Resolved Evanescent Wave-Induced Fluorescence Anisotropy Measurements. In: Geddes, C.D., Lakowicz, J.R. (eds) Reviews in Fluorescence 2005. Reviews in Fluorescence, vol 2005. Springer, Boston, MA. https://doi.org/10.1007/0-387-23690-2_11

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