Abstract
In mitochondria, there are many RNAs for which the function has yet to be fully characterized. In this paper, attention is focused on these poorly characterized RNAs and their possible role in regulating mitochondrial function. Using a cDNA clone made from a 0.2 kb L-strand transcript of mitochondrial regulatory region RNA (mrrlRNA) as a probe. we detected 1.3 kb RNAs from human tissues and cultured cells, and cloned cDNAs to them. Primary sequence studies suggest that they are transcribed from H-strandmitochondrialregulatory region DNA. Both the 1.3 kb RNA, refferredto as mrrH-RNA hereafter, and mrrL-RNA were detected in all human tissues and cultured cells examined.
With few exceptions, the level of 1.3 kb RNA is low in the cultured cells while it is high in tissue cells, and the level of 0.2 kb RNA is high in the cultured cells while it varies in tissue cells. Since one of the functions of mrrL-RNA is speculated to prime H-strand DNA synthesis, we propose that mrrH-RNA might function as an antisense RNA to inhibit mitochondrial H-strand DNA replication.
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© 2002 Kluwer Academic Publishers
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Nakamichi, N., Ito, M., Matsijmura, 1. (2002). Detection of Mitochondrial Regulatory Region RNA in Cultured Cells and Differentiated Tissue Cells: The Implications for Cellular Growth Control. In: Ikura, K., Nagao, M., Masuda, S., Sasaki, R. (eds) Animal Cell Technology: Challenges for the 21st Century. Springer, Dordrecht. https://doi.org/10.1007/0-306-46869-7_56
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DOI: https://doi.org/10.1007/0-306-46869-7_56
Publisher Name: Springer, Dordrecht
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