Regular ArticleCharacterisation of growth enhancing factor production in different phases of in vitro fish macrophage development
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2018, Developmental and Comparative ImmunologyCitation Excerpt :The PKM cultures consisted of heterogeneous populations of cells including early progenitors, monocytes and mature macrophages as determined by flow cytometry, morphology, cytochemistry and function. The 3–4-day cultures contained primarily monocytes, while older cultures (6–8 days of cultivation) were predominately mature macrophages (Barreda and Belosevic, 2001; Neumann et al., 2000). Goldfish splenocytes, neutrophils and PBLs were isolated as previously described (Grayfer and Belosevic, 2012; Katzenback and Belosevic, 2009; Xie et al., 2013; Xie and Belosevic, 2016).
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Corresponding author: CW 405 Biological Sciences Building, Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada. E-mail:[email protected]