Elsevier

Cytokine

Volume 11, Issue 9, September 1999, Pages 704-712
Cytokine

Regular Article
CXC CHEMOKINE RECEPTOR EXPRESSION ON HUMAN ENDOTHELIAL CELLS

https://doi.org/10.1006/cyto.1998.0465Get rights and content

Abstract

CXC Chemokines play a important role in the process of leukocyte recruitment and activation at sites of inflammation. However, recent evidence suggests that these molecules can also regulate endothelial cell functions such as migration, angiogenesis and proliferation. In this study we have investigated CXC chemokine receptor expression in both primary cultures of human umbilical vein endothelial cells (HUVEC) and the spontaneously transformed HUVEC cell line, ECV304. We found that both cell types express mRNA for chemokine receptors CXCR1, CXCR2 and CXCR4, but not CXCR3. Flow cytometric analysis revealed low levels of CXCR1 but higher levels of CXCR4 cell surface expression. HUVECs responded to SDF-1α with a rapid and robust calcium flux, however no calcium flux was seen with either IL-8 or Gro-α. HUVECs and ECV304 cells did not proliferate in response to CXC chemokines, although ECV304 cells did migrate towards SDF-1α and IL-8. These data demonstrate that HUVECs and the endothelial cell line, ECV304 express functional CXC chemokine receptors.

References (54)

  • CA Hebert et al.

    Scanning mutagenesis of interleukin-8 identifies a cluster of residues required for receptor-binding

    J Biol Chem

    ((1991))
  • P Petzelbauer et al.

    IL-8 and angiogenesis: evidence that human endothelial cells lack receptors and do not respond to IL-8 in vitro

    Cytokine

    ((1995))
  • SK Gupta et al.

    Chemokine receptors in human endothelial cells: functional expression of CXCR4 and its transcriptional regulation by inflammatory cytokines

    J Biol Chem

    ((1998))
  • C Murdoch et al.

    Chemokine and chemoattractant receptor expression on human endothelial cells

    Immunol Letts

    ((1997))
  • A Chaudhuri et al.

    Detection of Duffy antigen in the plasma membranes and caveolae of vascular endothelial and epithelial cells of nonerythroid organs

    Blood

    ((1997))
  • A Rot

    Endothelial cell binding of Nap-1/IL-8: role in neutrophil emigration

    Immunol Today

    ((1992))
  • M Shirozu et al.

    Structure and chromosomal location of the human stromal derived factor-1 (SDF-1)

    Genomics

    ((1995))
  • BJ Doranz et al.

    A dual-tropic, primary HIV-1 isolate that uses fusin and the β-chemokine receptors CKR-5, CKR-3 and CKR-2b as fusion cofactors

    Cell

    ((1996))
  • R Horuk et al.

    The CC chemokine I-309 inhibits CCR8-dependant infection by diverse HIV-1 strains

    J Biol Chem

    ((1998))
  • MJ Endres et al.

    CD4-independent infection by HIV-2 is mediated by fusin/CXCR4

    Cell

    ((1996))
  • P Proost et al.

    The role of chemokines in inflammation

    Int J Clin Lab Res

    ((1996))
  • M Baggiolini et al.

    Interleukin-8 and related chemotactic cytokines-CXC and CC chemokines

    Adv Immunol

    ((1994))
  • M Baggiolini et al.

    Human chemokines: an update

    Annu Rev Immunol

    ((1997))
  • GS Kelner et al.

    Lymphotactin: A cytokine that represents a new class of chemokines

    Science

    ((1994))
  • JF Bazan et al.

    A new class of membrane-bound chemokine with a CX3C motif

    Nature

    ((1997))
  • PM Murphy

    The molecular biology of leukocyte chemoattractant receptors

    Annu Rev Immunol

    ((1994))
  • WE Holmes et al.

    Structure and functional expression of a human interleukin-8 receptor

    Science

    ((1991))
  • Cited by (0)

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    Correspondence to: Craig Murdoch, Division of Child Health, Sheffield Children's Hospital, Western Bank, University of Sheffield, Sheffield S10 2TH, UK; E-mail:[email protected]

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