Regular ArticleRelation between the Activities Reducing Disulfides and the Protection against Membrane Permeability Transition in Rat Liver Mitochondria
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Acute emotional stress and high fat/high fructose diet modulate brain oxidative damage through NrF2 and uric acid in rats
2020, Nutrition ResearchCitation Excerpt :In brain mitochondria, our data confirm a significant increase in carbonyls and TBARS as markers of protein and lipid oxidation respectively. In addition, the decrease in TrxR activity in HF/HFr fed rats might alter the redox regulation of mitochondrial membrane permeability [45] leading to its impaired functional as we previously published for the same mitochondria batches [18]. In addition, since the glutaredoxin, as a member of the thioredoxin superfamily, is required to restore the S-glutathionylated proteins in mitochondria [46] decreased mitochondrial glutaredoxin GRX2 gene expression might aggravate the difficulty in maintenance of intracellular redox homeostasis in HF/HFr group.
Dietary supplementation of Spirulina ameliorates iron-induced oxidative stress in Indian knife fish Notopterus Notopterus
2018, Environmental Toxicology and PharmacologyCitation Excerpt :Oxidative stress indices in crude homogenate such as Lipid peroxidation (LPx) and protein carbonylation (PC) were assayed according to the method of Ohkawa et al. (1979) and Levine et al. (1994), respectively. Protein–sulfydryl (PSH) and non-protein–sulfydryl (NPSH) of crude homogenate were determined as per the methods of Sedlak and Lindsay (1968) and Wudarczyk et al. (1996). Activities of antioxidant enzymes such as superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione-s transferase (GST) were measured in sephadex-G-25 column elutes of PMF samples whereas catalase (CAT) was assayed directly in the PMF (Aebi, 1974) after incubating with 0.17 M ethanol on ice for 30 min followed by treatment with 1% (v/v) Triton-X-100 to inhibit Compound-I formation (Cohen et al., 1970).
Multivariate analysis of potential biomarkers of oxidative stress in Notopterus notopterus tissues from Mahanadi River as a function of concentration of heavy metals
2016, ChemosphereCitation Excerpt :Protein carbonyl content was measured in crude homogenate of tissue samples according to the method of Levine et al. (1994), Carbonyl content was calculated from its molar absorption coefficient as 22 × 103 M−1 cm−1 and results were expressed as nmol protein carbonyl formed mg−1 protein. Protein-SH and non-protein-SH content of crude homogenate of tissue samples were determined according to the method of Sedlak and Lindsay (1968) and Wudarczyk et al. (1996). Results were calculated from the standard curve of GSH and expressed in nmol thiol mg−1 protein.
T<inf>3</inf> fails to restore mitochondrial thiol redox status altered by experimental hypothyroidism in rat testis
2010, General and Comparative EndocrinologyCitation Excerpt :Protein carbonyl content was estimated in SMP and mitochondrial matrix fraction (Levine et al., 1990). SMP and matrix samples were precipitated in ice-cold 5% trichloroacetic acid containing 0.01 N HCl, centrifuged at 1000g for 15 min and the protein precipitates dissolved in 8 M guanidine hydrochloride were then used to measure protein-thiol content (Sedlak and Lindsay, 1968; Wudarczyk et al., 1996). Total glutathione content was measured in supernatant of the above-mentioned trichloroacetic acid-precipitated matrix samples by enzymatic recycling procedure and GSSG was measured after masking GSH with 2-vinylpyridine (Tietze, 1969; Griffith, 1980).
Mitochondria as targets in angiogenesis inhibition
2010, Molecular Aspects of MedicineWhat is the mitochondrial permeability transition pore?
2009, Journal of Molecular and Cellular Cardiology