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Title: Continuous enzymatic hydrolysis of lignocellulosic biomass in a membrane-reactor system: Continuous enzymatic hydrolysis of lignocellulosic biomass in a membrane-reactor system

Abstract

Abstract BACKGROUND Converting abundant lignocellulosic biomass to sugars as fungible precursors to fuels and chemicals has the potential to diversify the supply chain for those products, but further process improvements are needed to achieve economic viability. In the current work, process intensification of the key enzymatic hydrolysis unit operation is demonstrated by means of a membrane reactor system that was operated continuously. RESULTS Lignocellulosic biomass (pretreated corn stover) and buffered enzyme solution were fed to a continuously stirred‐tank reactor, and clarified sugar solution was withdrawn via a commercial tubular ultrafiltration membrane. The membrane permeance decline and membrane cleaning efficacy were studied and did not vary significantly when increasing fraction insoluble solids (FIS) from 2.5% to 5%. Continuous enzymatic hydrolysis was successfully operated for more than 80 h. A model for the reactor system was able to predict dynamic behavior that was in reasonable agreement with experimental results. CONCLUSION The modeled technical performance of anticipated commercial batch and continuous enzymatic hydrolysis processes were compared and showed that continuous operation would provide at least twice the volumetric productivity for the conditions studied. Further improvements are anticipated by better membrane selection and by increasing FIS. © 2017 Society of Chemical Industry

Authors:
ORCiD logo [1];  [2]; ORCiD logo [1];  [2]
  1. National Renewable Energy Lab. (NREL), Golden, CO (United States)
  2. Univ. of Colorado, Boulder, CO (United States)
Publication Date:
Research Org.:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office (BETO)
OSTI Identifier:
1426636
Alternate Identifier(s):
OSTI ID: 1422626
Report Number(s):
NREL/JA-5100-70067
Journal ID: ISSN 0268-2575
Grant/Contract Number:  
AC36-08GO28308; DE‐AC36‐08‐GO28308
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Journal of Chemical Technology and Biotechnology (1986)
Additional Journal Information:
Journal Volume: 93; Journal Issue: 5; Journal ID: ISSN 0268-2575
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; enzymatic hydrolysis; lignocellulose; continuous reaction; membrane reactor; process intensification

Citation Formats

Stickel, Jonathan J., Adhikari, Birendra, Sievers, David A., and Pellegrino, John. Continuous enzymatic hydrolysis of lignocellulosic biomass in a membrane-reactor system: Continuous enzymatic hydrolysis of lignocellulosic biomass in a membrane-reactor system. United States: N. p., 2018. Web. doi:10.1002/jctb.5559.
Stickel, Jonathan J., Adhikari, Birendra, Sievers, David A., & Pellegrino, John. Continuous enzymatic hydrolysis of lignocellulosic biomass in a membrane-reactor system: Continuous enzymatic hydrolysis of lignocellulosic biomass in a membrane-reactor system. United States. https://doi.org/10.1002/jctb.5559
Stickel, Jonathan J., Adhikari, Birendra, Sievers, David A., and Pellegrino, John. 2018. "Continuous enzymatic hydrolysis of lignocellulosic biomass in a membrane-reactor system: Continuous enzymatic hydrolysis of lignocellulosic biomass in a membrane-reactor system". United States. https://doi.org/10.1002/jctb.5559. https://www.osti.gov/servlets/purl/1426636.
@article{osti_1426636,
title = {Continuous enzymatic hydrolysis of lignocellulosic biomass in a membrane-reactor system: Continuous enzymatic hydrolysis of lignocellulosic biomass in a membrane-reactor system},
author = {Stickel, Jonathan J. and Adhikari, Birendra and Sievers, David A. and Pellegrino, John},
abstractNote = {Abstract BACKGROUND Converting abundant lignocellulosic biomass to sugars as fungible precursors to fuels and chemicals has the potential to diversify the supply chain for those products, but further process improvements are needed to achieve economic viability. In the current work, process intensification of the key enzymatic hydrolysis unit operation is demonstrated by means of a membrane reactor system that was operated continuously. RESULTS Lignocellulosic biomass (pretreated corn stover) and buffered enzyme solution were fed to a continuously stirred‐tank reactor, and clarified sugar solution was withdrawn via a commercial tubular ultrafiltration membrane. The membrane permeance decline and membrane cleaning efficacy were studied and did not vary significantly when increasing fraction insoluble solids (FIS) from 2.5% to 5%. Continuous enzymatic hydrolysis was successfully operated for more than 80 h. A model for the reactor system was able to predict dynamic behavior that was in reasonable agreement with experimental results. CONCLUSION The modeled technical performance of anticipated commercial batch and continuous enzymatic hydrolysis processes were compared and showed that continuous operation would provide at least twice the volumetric productivity for the conditions studied. Further improvements are anticipated by better membrane selection and by increasing FIS. © 2017 Society of Chemical Industry},
doi = {10.1002/jctb.5559},
url = {https://www.osti.gov/biblio/1426636}, journal = {Journal of Chemical Technology and Biotechnology (1986)},
issn = {0268-2575},
number = 5,
volume = 93,
place = {United States},
year = {Wed Feb 21 00:00:00 EST 2018},
month = {Wed Feb 21 00:00:00 EST 2018}
}

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Cited by: 15 works
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Works referenced in this record:

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journal, October 2016


Reactor design for minimizing product inhibition during enzymatic lignocellulose hydrolysis
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Particle concentration and yield stress of biomass slurries during enzymatic hydrolysis at high-solids loadings
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  • Kumar, Parveen; Barrett, Diane M.; Delwiche, Michael J.
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Works referencing / citing this record:

A two-phase substrate model for enzymatic hydrolysis of lignocellulose: application to batch and continuous reactors
journal, December 2019